A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis
A comprehensive research has been carried out on L-glutaminase producers from marine bacteria. Among the evaluated isolates, Kosakonia radicincitans was found to be a L-glutaminase producer from a marine environment, having the highest enzymatic activities. The optimal condition for L-glutaminase pr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Springer International Publishing
2021
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/34064/1/A%20new%20L-glutaminase%20from%20Kosakonia%20sp..pdf |
| _version_ | 1796995079742685184 |
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| author | Aizi Nor Mazila, Ramli Nur Dini, Johari Mohd Akmal, Azhar Rohaida, Che Man Hazrulrizawati, Hamid |
| author_facet | Aizi Nor Mazila, Ramli Nur Dini, Johari Mohd Akmal, Azhar Rohaida, Che Man Hazrulrizawati, Hamid |
| author_sort | Aizi Nor Mazila, Ramli |
| collection | UMP |
| description | A comprehensive research has been carried out on L-glutaminase producers from marine bacteria. Among the evaluated isolates, Kosakonia radicincitans was found to be a L-glutaminase producer from a marine environment, having the highest enzymatic activities. The optimal condition for L-glutaminase production from K. radicincitans was at pH and temperature of 7.0 and 37 °C, respectively. The enzyme was found stable between pH 4 and 8 and its function also maintained at temperatures between 31 and 43 °C after 30-min incubation. Both the amino acid sequence and domain analysis of the L-glutaminase from the Kosakonia strains revealed various similarities and dissimilarities between these amidase enzymes. Sequence analysis and comparison of L-glutaminase from Kosakonia sp. with other L-glutaminase along with structural prediction were able to identify the unique characteristics of the predicted structures and offer a different perspective into their distinguishable properties for industrial use. |
| first_indexed | 2024-03-06T12:57:02Z |
| format | Article |
| id | UMPir34064 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:57:02Z |
| publishDate | 2021 |
| publisher | Springer International Publishing |
| record_format | dspace |
| spelling | UMPir340642022-05-12T02:37:51Z http://umpir.ump.edu.my/id/eprint/34064/ A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis Aizi Nor Mazila, Ramli Nur Dini, Johari Mohd Akmal, Azhar Rohaida, Che Man Hazrulrizawati, Hamid Q Science (General) A comprehensive research has been carried out on L-glutaminase producers from marine bacteria. Among the evaluated isolates, Kosakonia radicincitans was found to be a L-glutaminase producer from a marine environment, having the highest enzymatic activities. The optimal condition for L-glutaminase production from K. radicincitans was at pH and temperature of 7.0 and 37 °C, respectively. The enzyme was found stable between pH 4 and 8 and its function also maintained at temperatures between 31 and 43 °C after 30-min incubation. Both the amino acid sequence and domain analysis of the L-glutaminase from the Kosakonia strains revealed various similarities and dissimilarities between these amidase enzymes. Sequence analysis and comparison of L-glutaminase from Kosakonia sp. with other L-glutaminase along with structural prediction were able to identify the unique characteristics of the predicted structures and offer a different perspective into their distinguishable properties for industrial use. Springer International Publishing 2021 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/34064/1/A%20new%20L-glutaminase%20from%20Kosakonia%20sp..pdf Aizi Nor Mazila, Ramli and Nur Dini, Johari and Mohd Akmal, Azhar and Rohaida, Che Man and Hazrulrizawati, Hamid (2021) A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis. Journal of Food Measurement and Characterization, 15. pp. 862-875. ISSN 2193-4126 (Print); 2193-4134 (Online). (Published) https://doi.org/10.1007/s11694-020-00682-z https://doi.org/10.1007/s11694-020-00682-z |
| spellingShingle | Q Science (General) Aizi Nor Mazila, Ramli Nur Dini, Johari Mohd Akmal, Azhar Rohaida, Che Man Hazrulrizawati, Hamid A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis |
| title | A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis |
| title_full | A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis |
| title_fullStr | A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis |
| title_full_unstemmed | A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis |
| title_short | A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis |
| title_sort | new l glutaminase from kosakonia sp extracellular production gene identification and structural analysis |
| topic | Q Science (General) |
| url | http://umpir.ump.edu.my/id/eprint/34064/1/A%20new%20L-glutaminase%20from%20Kosakonia%20sp..pdf |
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