The extraction of papain from papaya leaves
The use of papaya leaves to extract papain enzyme was evaluated for different extraction technique mainly using grinding method, enzyme-assisted extraction and ultrasonication. Enzyme concentration was then determined by measuring the amount of amino acid released in the reaction of protease assay....
| Main Author: | |
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| Format: | Undergraduates Project Papers |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/3428/1/CD5957_Nur_Dianaty_Nordin.pdf |
| _version_ | 1825821392045604864 |
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| author | Nur Dianaty, Nordin |
| author_facet | Nur Dianaty, Nordin |
| author_sort | Nur Dianaty, Nordin |
| collection | UMP |
| description | The use of papaya leaves to extract papain enzyme was evaluated for different extraction technique mainly using grinding method, enzyme-assisted extraction and ultrasonication. Enzyme concentration was then determined by measuring the amount of amino acid released in the reaction of protease assay. The combination of grinding method, enzyme-assisted extraction was proven to give highest yield of papain by 36.75% when compared to grinding alone. Then further purification of the extracted papain was done by using ammonium sulfate precipitation. The optimized concentration of ammonium sulfate saturation was achieved at 55% concentration in order to fully precipitate the protein. The combination of grinding, enzyme-assisted extraction and ultrasonication can be considered as a potential extraction technique to extract papain as it is more delicate towards protein extraction. Furthermore, the investigation on other source of papain such as from the petioles, stalks and stem could also be done. |
| first_indexed | 2024-03-06T11:40:50Z |
| format | Undergraduates Project Papers |
| id | UMPir3428 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T11:40:50Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | UMPir34282021-07-05T04:43:33Z http://umpir.ump.edu.my/id/eprint/3428/ The extraction of papain from papaya leaves Nur Dianaty, Nordin TP Chemical technology The use of papaya leaves to extract papain enzyme was evaluated for different extraction technique mainly using grinding method, enzyme-assisted extraction and ultrasonication. Enzyme concentration was then determined by measuring the amount of amino acid released in the reaction of protease assay. The combination of grinding method, enzyme-assisted extraction was proven to give highest yield of papain by 36.75% when compared to grinding alone. Then further purification of the extracted papain was done by using ammonium sulfate precipitation. The optimized concentration of ammonium sulfate saturation was achieved at 55% concentration in order to fully precipitate the protein. The combination of grinding, enzyme-assisted extraction and ultrasonication can be considered as a potential extraction technique to extract papain as it is more delicate towards protein extraction. Furthermore, the investigation on other source of papain such as from the petioles, stalks and stem could also be done. 2010-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3428/1/CD5957_Nur_Dianaty_Nordin.pdf Nur Dianaty, Nordin (2010) The extraction of papain from papaya leaves. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. |
| spellingShingle | TP Chemical technology Nur Dianaty, Nordin The extraction of papain from papaya leaves |
| title | The extraction of papain from papaya leaves |
| title_full | The extraction of papain from papaya leaves |
| title_fullStr | The extraction of papain from papaya leaves |
| title_full_unstemmed | The extraction of papain from papaya leaves |
| title_short | The extraction of papain from papaya leaves |
| title_sort | extraction of papain from papaya leaves |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/3428/1/CD5957_Nur_Dianaty_Nordin.pdf |
| work_keys_str_mv | AT nurdianatynordin theextractionofpapainfrompapayaleaves AT nurdianatynordin extractionofpapainfrompapayaleaves |