Magnetic nickel nanostructure as cellulase immobilization surface for the hydrolysis of lignocellulosic biomass
In this research, a magnetic reusable nickel nanoparticle (NiNPs) supporting materials were prepared for cellulase enzyme immobilization. The immobilized cellulase showed high activity recovery, large & fast immobilization capacity and improved pH & temperature tolerance. The excellent stabi...
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| Format: | Article |
| Language: | English English |
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Elsevier Ltd
2022
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| Online Access: | http://umpir.ump.edu.my/id/eprint/34754/1/Magnetic%20nickel%20nanostructure%20as%20cellulase%20immobilization%20surface_FULL.pdf http://umpir.ump.edu.my/id/eprint/34754/2/Magnetic%20nickel%20nanostructure%20as%20cellulase%20immobilization%20surface%20.pdf |
| _version_ | 1825824094718787584 |
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| author | Rashid, Shah Samiur Mustafa, Abu Hasnat Mohd Hasbi, Ab. Rahim Gunes, Burcu |
| author_facet | Rashid, Shah Samiur Mustafa, Abu Hasnat Mohd Hasbi, Ab. Rahim Gunes, Burcu |
| author_sort | Rashid, Shah Samiur |
| collection | UMP |
| description | In this research, a magnetic reusable nickel nanoparticle (NiNPs) supporting materials were prepared for cellulase enzyme immobilization. The immobilized cellulase showed high activity recovery, large & fast immobilization capacity and improved pH & temperature tolerance. The excellent stability and reusability enabled the immobilized cellulase to retain 84% of its initial activity after ten cycles. At 2 mg/mL enzyme concentration, highest 93% immobilization efficiency was achieved within two hours of immobilization. When the treatment temperature reached 40 °C and pH 5, the immobilized cellulase exhibited highest residual activity. The immobilized cellulase could be separated from the solution by a magnetic force. This study introduced a novel supporting material for cellulase immobilization, and the immobilized cellulase poses a great potential in the hydrolysis of lignocellulosic biomass which can used as an easily applicable and sustainable pre-treatment step for advanced biofuel production. |
| first_indexed | 2024-03-06T12:58:57Z |
| format | Article |
| id | UMPir34754 |
| institution | Universiti Malaysia Pahang |
| language | English English |
| last_indexed | 2024-03-06T12:58:57Z |
| publishDate | 2022 |
| publisher | Elsevier Ltd |
| record_format | dspace |
| spelling | UMPir347542022-07-21T05:01:14Z http://umpir.ump.edu.my/id/eprint/34754/ Magnetic nickel nanostructure as cellulase immobilization surface for the hydrolysis of lignocellulosic biomass Rashid, Shah Samiur Mustafa, Abu Hasnat Mohd Hasbi, Ab. Rahim Gunes, Burcu TP Chemical technology In this research, a magnetic reusable nickel nanoparticle (NiNPs) supporting materials were prepared for cellulase enzyme immobilization. The immobilized cellulase showed high activity recovery, large & fast immobilization capacity and improved pH & temperature tolerance. The excellent stability and reusability enabled the immobilized cellulase to retain 84% of its initial activity after ten cycles. At 2 mg/mL enzyme concentration, highest 93% immobilization efficiency was achieved within two hours of immobilization. When the treatment temperature reached 40 °C and pH 5, the immobilized cellulase exhibited highest residual activity. The immobilized cellulase could be separated from the solution by a magnetic force. This study introduced a novel supporting material for cellulase immobilization, and the immobilized cellulase poses a great potential in the hydrolysis of lignocellulosic biomass which can used as an easily applicable and sustainable pre-treatment step for advanced biofuel production. Elsevier Ltd 2022-06 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/34754/1/Magnetic%20nickel%20nanostructure%20as%20cellulase%20immobilization%20surface_FULL.pdf pdf en http://umpir.ump.edu.my/id/eprint/34754/2/Magnetic%20nickel%20nanostructure%20as%20cellulase%20immobilization%20surface%20.pdf Rashid, Shah Samiur and Mustafa, Abu Hasnat and Mohd Hasbi, Ab. Rahim and Gunes, Burcu (2022) Magnetic nickel nanostructure as cellulase immobilization surface for the hydrolysis of lignocellulosic biomass. International Journal of Biological Macromolecules, 209. 1048 -1053. ISSN 0141-8130. (Published) https://doi.org/10.1016/j.ijbiomac.2022.04.072 https://doi.org/10.1016/j.ijbiomac.2022.04.072 |
| spellingShingle | TP Chemical technology Rashid, Shah Samiur Mustafa, Abu Hasnat Mohd Hasbi, Ab. Rahim Gunes, Burcu Magnetic nickel nanostructure as cellulase immobilization surface for the hydrolysis of lignocellulosic biomass |
| title | Magnetic nickel nanostructure as cellulase immobilization surface for the hydrolysis of lignocellulosic biomass |
| title_full | Magnetic nickel nanostructure as cellulase immobilization surface for the hydrolysis of lignocellulosic biomass |
| title_fullStr | Magnetic nickel nanostructure as cellulase immobilization surface for the hydrolysis of lignocellulosic biomass |
| title_full_unstemmed | Magnetic nickel nanostructure as cellulase immobilization surface for the hydrolysis of lignocellulosic biomass |
| title_short | Magnetic nickel nanostructure as cellulase immobilization surface for the hydrolysis of lignocellulosic biomass |
| title_sort | magnetic nickel nanostructure as cellulase immobilization surface for the hydrolysis of lignocellulosic biomass |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/34754/1/Magnetic%20nickel%20nanostructure%20as%20cellulase%20immobilization%20surface_FULL.pdf http://umpir.ump.edu.my/id/eprint/34754/2/Magnetic%20nickel%20nanostructure%20as%20cellulase%20immobilization%20surface%20.pdf |
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