Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia
Antibiotic contamination in the marine environment forms an emerging threat to marine ecosystems. This study aimed to compare the gut and coelomic microbiota of Stichopus ocellatus with sediments between two coastal districts of Pahang, which potentially conferring as putative biomarkers for sedimen...
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| Format: | Article |
| Language: | English English |
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Elsevier
2022
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| Online Access: | http://umpir.ump.edu.my/id/eprint/34904/1/1-s2.0-S0025326X22007044-main.pdf http://umpir.ump.edu.my/id/eprint/34904/7/Gut%20microbiome%20and%20metabolome%20of%20sea%20cucumber%20.pdf |
| _version_ | 1796995238635503616 |
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| author | Siew, Shing Wei Choo, Mei Yen Marshall, Ian P. G. Abd Hamid, Hazrulrizawati Kamal, Shamrulazhar Shamzir Nielsen, Dennis Sandris Ahmad, Hajar Fauzan |
| author_facet | Siew, Shing Wei Choo, Mei Yen Marshall, Ian P. G. Abd Hamid, Hazrulrizawati Kamal, Shamrulazhar Shamzir Nielsen, Dennis Sandris Ahmad, Hajar Fauzan |
| author_sort | Siew, Shing Wei |
| collection | UMP |
| description | Antibiotic contamination in the marine environment forms an emerging threat to marine ecosystems. This study aimed to compare the gut and coelomic microbiota of Stichopus ocellatus with sediments between two coastal districts of Pahang, which potentially conferring as putative biomarkers for sediment pollution monitoring. The composition of the bacteria communities was determined using 16S rRNA V3-region gene amplicon sequencing, while hybrid whole-genome sequencing was employed to analyze the genome of Vibrio parahaemolyticus. The trace elements and antibiotic compositions were access using high throughput spectrometry. The alpha- and beta-diversity of bacteria in gut and sediment samples from Kuantan differed substantially within (p-value = 0.017604) and between samples (p-value <0.007), respectively. Vibrio genera predominated in Kuantan samples, while Flavobacterium and Synechococcus_E genera predominated in Pekan samples. Vibrio parahaemolyticus revealed the presence of tet (35) and blaCARB-33 genes that conceived resistance towards tetracycline and beta-lactam antibiotics, respectively, which were detected in sediment and gut samples. |
| first_indexed | 2024-03-06T12:59:22Z |
| format | Article |
| id | UMPir34904 |
| institution | Universiti Malaysia Pahang |
| language | English English |
| last_indexed | 2024-03-06T12:59:22Z |
| publishDate | 2022 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | UMPir349042022-10-07T03:27:17Z http://umpir.ump.edu.my/id/eprint/34904/ Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia Siew, Shing Wei Choo, Mei Yen Marshall, Ian P. G. Abd Hamid, Hazrulrizawati Kamal, Shamrulazhar Shamzir Nielsen, Dennis Sandris Ahmad, Hajar Fauzan QE Geology QR Microbiology Antibiotic contamination in the marine environment forms an emerging threat to marine ecosystems. This study aimed to compare the gut and coelomic microbiota of Stichopus ocellatus with sediments between two coastal districts of Pahang, which potentially conferring as putative biomarkers for sediment pollution monitoring. The composition of the bacteria communities was determined using 16S rRNA V3-region gene amplicon sequencing, while hybrid whole-genome sequencing was employed to analyze the genome of Vibrio parahaemolyticus. The trace elements and antibiotic compositions were access using high throughput spectrometry. The alpha- and beta-diversity of bacteria in gut and sediment samples from Kuantan differed substantially within (p-value = 0.017604) and between samples (p-value <0.007), respectively. Vibrio genera predominated in Kuantan samples, while Flavobacterium and Synechococcus_E genera predominated in Pekan samples. Vibrio parahaemolyticus revealed the presence of tet (35) and blaCARB-33 genes that conceived resistance towards tetracycline and beta-lactam antibiotics, respectively, which were detected in sediment and gut samples. Elsevier 2022-09 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/34904/1/1-s2.0-S0025326X22007044-main.pdf pdf en http://umpir.ump.edu.my/id/eprint/34904/7/Gut%20microbiome%20and%20metabolome%20of%20sea%20cucumber%20.pdf Siew, Shing Wei and Choo, Mei Yen and Marshall, Ian P. G. and Abd Hamid, Hazrulrizawati and Kamal, Shamrulazhar Shamzir and Nielsen, Dennis Sandris and Ahmad, Hajar Fauzan (2022) Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia. Marine Pollution Bulletin, 182 (114022). pp. 1-15. ISSN 0025-326X. (Published) https://doi.org/10.1016/j.marpolbul.2022.114022 https://doi.org/10.1016/j.marpolbul.2022.114022 |
| spellingShingle | QE Geology QR Microbiology Siew, Shing Wei Choo, Mei Yen Marshall, Ian P. G. Abd Hamid, Hazrulrizawati Kamal, Shamrulazhar Shamzir Nielsen, Dennis Sandris Ahmad, Hajar Fauzan Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia |
| title | Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia |
| title_full | Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia |
| title_fullStr | Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia |
| title_full_unstemmed | Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia |
| title_short | Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia |
| title_sort | gut microbiome and metabolome of sea cucumber stichopus ocellatus as putative markers for monitoring the marine sediment pollution in pahang malaysia |
| topic | QE Geology QR Microbiology |
| url | http://umpir.ump.edu.my/id/eprint/34904/1/1-s2.0-S0025326X22007044-main.pdf http://umpir.ump.edu.my/id/eprint/34904/7/Gut%20microbiome%20and%20metabolome%20of%20sea%20cucumber%20.pdf |
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