Screening of significant factors affecting pravastatin production by Penicillium sp. ESF21P
Pravastatin is a clinically useful cholesterol-lowering agent. The development of a one-step fermentation process using pravastatin-producing microfungi may be an attractive approach from an economic point of view. To facilitate this, previously 54 fungal cultures were isolated from soil samples. Am...
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| Format: | Conference or Workshop Item |
| Language: | English |
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Institute of Physics Publishing
2020
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/35973/1/Screening%20of%20significant%20factors%20affecting%20pravastatin%20production%20by%20Penicillium%20sp.%20ESF21P.pdf |
| _version_ | 1825814694598803456 |
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| author | Norazwina, Zainol Seydametova, Emine Jailani, Salihon Convey, Peter |
| author_facet | Norazwina, Zainol Seydametova, Emine Jailani, Salihon Convey, Peter |
| author_sort | Norazwina, Zainol |
| collection | UMP |
| description | Pravastatin is a clinically useful cholesterol-lowering agent. The development of a one-step fermentation process using pravastatin-producing microfungi may be an attractive approach from an economic point of view. To facilitate this, previously 54 fungal cultures were isolated from soil samples. Among them, Penicillium sp. ESF21P was the most active pravastatin producer (196.83 mg/L). The objective of the present study is to determine significant factors affecting pravastatin production by Penicillium sp. ESF21P. The method of the 27-3 fractional factorial design with seven variables was performed using Design-Expert 6.0.8 software package. The seven factors studied were slant age, spore concentration, inoculum volume, fermentation time, temperature, initial pH of the medium, and agitation rate. The results obtained confirmed that the factorial model was significant. Amongst the tested factors, only four were important: agitation rate, slant age, initial pH of the medium, and fermentation time with a percentage contribution of 25.66%, 11.56%, 9.72%, and 7.69%, respectively. These significant factors will be optimized further using response surface methodology. |
| first_indexed | 2024-03-06T13:02:22Z |
| format | Conference or Workshop Item |
| id | UMPir35973 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T13:02:22Z |
| publishDate | 2020 |
| publisher | Institute of Physics Publishing |
| record_format | dspace |
| spelling | UMPir359732022-12-28T04:51:02Z http://umpir.ump.edu.my/id/eprint/35973/ Screening of significant factors affecting pravastatin production by Penicillium sp. ESF21P Norazwina, Zainol Seydametova, Emine Jailani, Salihon Convey, Peter QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Pravastatin is a clinically useful cholesterol-lowering agent. The development of a one-step fermentation process using pravastatin-producing microfungi may be an attractive approach from an economic point of view. To facilitate this, previously 54 fungal cultures were isolated from soil samples. Among them, Penicillium sp. ESF21P was the most active pravastatin producer (196.83 mg/L). The objective of the present study is to determine significant factors affecting pravastatin production by Penicillium sp. ESF21P. The method of the 27-3 fractional factorial design with seven variables was performed using Design-Expert 6.0.8 software package. The seven factors studied were slant age, spore concentration, inoculum volume, fermentation time, temperature, initial pH of the medium, and agitation rate. The results obtained confirmed that the factorial model was significant. Amongst the tested factors, only four were important: agitation rate, slant age, initial pH of the medium, and fermentation time with a percentage contribution of 25.66%, 11.56%, 9.72%, and 7.69%, respectively. These significant factors will be optimized further using response surface methodology. Institute of Physics Publishing 2020-03-04 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/35973/1/Screening%20of%20significant%20factors%20affecting%20pravastatin%20production%20by%20Penicillium%20sp.%20ESF21P.pdf Norazwina, Zainol and Seydametova, Emine and Jailani, Salihon and Convey, Peter (2020) Screening of significant factors affecting pravastatin production by Penicillium sp. ESF21P. In: IOP Conference Series: Materials Science and Engineering, Energy Security and Chemical Engineering Congress , 17-19 July 2019 , Kuala Lumpur, Malaysia. pp. 1-6., 736 (022087). ISSN 1757-8981 (Published) https://doi.org/10.1088/1757-899X/736/2/022087 |
| spellingShingle | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Norazwina, Zainol Seydametova, Emine Jailani, Salihon Convey, Peter Screening of significant factors affecting pravastatin production by Penicillium sp. ESF21P |
| title | Screening of significant factors affecting pravastatin production by Penicillium sp. ESF21P |
| title_full | Screening of significant factors affecting pravastatin production by Penicillium sp. ESF21P |
| title_fullStr | Screening of significant factors affecting pravastatin production by Penicillium sp. ESF21P |
| title_full_unstemmed | Screening of significant factors affecting pravastatin production by Penicillium sp. ESF21P |
| title_short | Screening of significant factors affecting pravastatin production by Penicillium sp. ESF21P |
| title_sort | screening of significant factors affecting pravastatin production by penicillium sp esf21p |
| topic | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/35973/1/Screening%20of%20significant%20factors%20affecting%20pravastatin%20production%20by%20Penicillium%20sp.%20ESF21P.pdf |
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