Screening of extraction buffer concentration for protease extraction from the leaves of Syzygium polyanthum using full factorial design
This study aimed to identify the factors that significantly affect the extraction of protease from the leaves of Syzygium polyanthum. The extraction of protease from the leaves of Syzigium polyanthum was studied at different concentration of substances in the extraction buffer. A 24 full factorial e...
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| Format: | Conference or Workshop Item |
| Language: | English |
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Institute of Physics Publishing
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/36011/1/Screening%20of%20extraction%20buffer%20concentration%20for%20protease%20extraction%20from%20the%20leaves%20of%20Syzygium%20polyanthum%20using%20full%20factorial%20design.pdf |
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| author | Noorhafiza Diyana, Yahya Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Rohaida, Che Man Shalyda, Md Shaarani @ Md Nawi Mohd Hairul, Ab. Rahim |
| author_facet | Noorhafiza Diyana, Yahya Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Rohaida, Che Man Shalyda, Md Shaarani @ Md Nawi Mohd Hairul, Ab. Rahim |
| author_sort | Noorhafiza Diyana, Yahya |
| collection | UMP |
| description | This study aimed to identify the factors that significantly affect the extraction of protease from the leaves of Syzygium polyanthum. The extraction of protease from the leaves of Syzigium polyanthum was studied at different concentration of substances in the extraction buffer. A 24 full factorial experimental design was performed. The effect of four independent operating factors including; A, the concentration of potassium phosphate (KPO4); B, the concentration of Triton X-100; C, the concentration of glycerol; and D, the concentration of dithiotreitol (DTT) on the protease activity were evaluated statistically. All regression models gave a good fit to the experimental data (R2 > 99%). The maximum protease activity was detected when the extraction was performed using 3.0 M KPO4, 1% Triton X-100, 80% glycerol and 1.5 M DTT. The results revealed that the concentration of KPO4, DTT, and their interaction were statistically significant to affect the protease activity. Thus, the results obtained from this study indicate that full factorial design is useful to screen the significant factors and minimise the number of experiments. |
| first_indexed | 2024-03-06T13:02:27Z |
| format | Conference or Workshop Item |
| id | UMPir36011 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T13:02:27Z |
| publishDate | 2020 |
| publisher | Institute of Physics Publishing |
| record_format | dspace |
| spelling | UMPir360112022-12-28T01:38:43Z http://umpir.ump.edu.my/id/eprint/36011/ Screening of extraction buffer concentration for protease extraction from the leaves of Syzygium polyanthum using full factorial design Noorhafiza Diyana, Yahya Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Rohaida, Che Man Shalyda, Md Shaarani @ Md Nawi Mohd Hairul, Ab. Rahim QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology This study aimed to identify the factors that significantly affect the extraction of protease from the leaves of Syzygium polyanthum. The extraction of protease from the leaves of Syzigium polyanthum was studied at different concentration of substances in the extraction buffer. A 24 full factorial experimental design was performed. The effect of four independent operating factors including; A, the concentration of potassium phosphate (KPO4); B, the concentration of Triton X-100; C, the concentration of glycerol; and D, the concentration of dithiotreitol (DTT) on the protease activity were evaluated statistically. All regression models gave a good fit to the experimental data (R2 > 99%). The maximum protease activity was detected when the extraction was performed using 3.0 M KPO4, 1% Triton X-100, 80% glycerol and 1.5 M DTT. The results revealed that the concentration of KPO4, DTT, and their interaction were statistically significant to affect the protease activity. Thus, the results obtained from this study indicate that full factorial design is useful to screen the significant factors and minimise the number of experiments. Institute of Physics Publishing 2020-03-04 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/36011/1/Screening%20of%20extraction%20buffer%20concentration%20for%20protease%20extraction%20from%20the%20leaves%20of%20Syzygium%20polyanthum%20using%20full%20factorial%20design.pdf Noorhafiza Diyana, Yahya and Zatul Iffah, Mohd Arshad and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman and Rohaida, Che Man and Shalyda, Md Shaarani @ Md Nawi and Mohd Hairul, Ab. Rahim (2020) Screening of extraction buffer concentration for protease extraction from the leaves of Syzygium polyanthum using full factorial design. In: IOP Conference Series: Materials Science and Engineering, Energy Security and Chemical Engineering Congress , 17-19 July 2019 , Kuala Lumpur, Malaysia. pp. 1-10., 736 (022082). ISSN 1757-8981 (Published) https://doi.org/10.1088/1757-899X/736/2/022082 |
| spellingShingle | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Noorhafiza Diyana, Yahya Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Rohaida, Che Man Shalyda, Md Shaarani @ Md Nawi Mohd Hairul, Ab. Rahim Screening of extraction buffer concentration for protease extraction from the leaves of Syzygium polyanthum using full factorial design |
| title | Screening of extraction buffer concentration for protease extraction from the leaves of Syzygium polyanthum using full factorial design |
| title_full | Screening of extraction buffer concentration for protease extraction from the leaves of Syzygium polyanthum using full factorial design |
| title_fullStr | Screening of extraction buffer concentration for protease extraction from the leaves of Syzygium polyanthum using full factorial design |
| title_full_unstemmed | Screening of extraction buffer concentration for protease extraction from the leaves of Syzygium polyanthum using full factorial design |
| title_short | Screening of extraction buffer concentration for protease extraction from the leaves of Syzygium polyanthum using full factorial design |
| title_sort | screening of extraction buffer concentration for protease extraction from the leaves of syzygium polyanthum using full factorial design |
| topic | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/36011/1/Screening%20of%20extraction%20buffer%20concentration%20for%20protease%20extraction%20from%20the%20leaves%20of%20Syzygium%20polyanthum%20using%20full%20factorial%20design.pdf |
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