qPCR analysis of quorum sensing genes of pseudomonas aeruginosa: Primer design and qPCR efficiency
A well-designed and properly validated primer produces a specific and efficient qPCR assay. The primer’s melting temperature, G-C content, its size, as well as the amplicon’s size are the principal considerations while designing the primer for this study and it was in accordance with the MIQE guidel...
Main Authors: | Saiedatul Akmal, Mohamed Sedek, Mohd Azmir, Arifin, Mimi Sakinah, Abdul Munaim |
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Format: | Article |
Language: | English |
Published: |
Penerbit Universiti Malaysia Pahang
2023
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Subjects: | |
Online Access: | http://umpir.ump.edu.my/id/eprint/37835/1/qPCR%20Analysis%20of%20Quorum%20Sensing%20Genes%20of%20Pseudomonas%20aeruginosa.pdf |
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