Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study
An analytical method based on solid-phase extraction (SPE) liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the determination of five illicit drugs, namely amphetamine (AM), methamphetamine (MA), 3,4- methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), and morphin...
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| Format: | Article |
| Language: | English |
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Universiti Kebangsaan Malaysia
2023
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| Online Access: | http://umpir.ump.edu.my/id/eprint/37910/1/Izani_27_1_1.pdf |
| _version_ | 1825815018950623232 |
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| author | Mohammad Azimuddin, Izani Nurul `Azyyati, Sabri Nurul Nadiah, Hamidon Kooi, Yeong Khaw Iekhsan, Othman Mueller, Jochen Tay, Joo Hui |
| author_facet | Mohammad Azimuddin, Izani Nurul `Azyyati, Sabri Nurul Nadiah, Hamidon Kooi, Yeong Khaw Iekhsan, Othman Mueller, Jochen Tay, Joo Hui |
| author_sort | Mohammad Azimuddin, Izani |
| collection | UMP |
| description | An analytical method based on solid-phase extraction (SPE) liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the determination of five illicit drugs, namely amphetamine (AM), methamphetamine (MA), 3,4- methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), and morphine (MOR) in municipal wastewater has been optimized and validated. Sample preparation was performed using Oasis MCX SPE cartridges. LC separation was performed using a Zorbax Eclipse Plus C18 RRHD column. The linearity of the calibration curve was between 5 ng/mL and 250 ng/mL, with determination coefficient (R2 ) greater than 0.99, except for morphine. The mean recoveries of target analytes ranged from 91.6 to 112%, and the method demonstrated good inter-day repeatability (coefficient of variation, CV ranged from 2 to 19%). The limit of detection (LOD) for AM, MA, MDMA, MDA, and MOR was 0.29, 0.37, 0.86, 1.09 and 7.56 ng/mL, respectively. The method was applied to municipal wastewater samples collected from sewage treatment plants in Kuantan, Pahang, in which AM, MA and MDA were detected in all 3 samples. |
| first_indexed | 2024-03-06T13:07:12Z |
| format | Article |
| id | UMPir37910 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T13:07:12Z |
| publishDate | 2023 |
| publisher | Universiti Kebangsaan Malaysia |
| record_format | dspace |
| spelling | UMPir379102023-07-13T07:14:55Z http://umpir.ump.edu.my/id/eprint/37910/ Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study Mohammad Azimuddin, Izani Nurul `Azyyati, Sabri Nurul Nadiah, Hamidon Kooi, Yeong Khaw Iekhsan, Othman Mueller, Jochen Tay, Joo Hui QD Chemistry TD Environmental technology. Sanitary engineering TP Chemical technology An analytical method based on solid-phase extraction (SPE) liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the determination of five illicit drugs, namely amphetamine (AM), methamphetamine (MA), 3,4- methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), and morphine (MOR) in municipal wastewater has been optimized and validated. Sample preparation was performed using Oasis MCX SPE cartridges. LC separation was performed using a Zorbax Eclipse Plus C18 RRHD column. The linearity of the calibration curve was between 5 ng/mL and 250 ng/mL, with determination coefficient (R2 ) greater than 0.99, except for morphine. The mean recoveries of target analytes ranged from 91.6 to 112%, and the method demonstrated good inter-day repeatability (coefficient of variation, CV ranged from 2 to 19%). The limit of detection (LOD) for AM, MA, MDMA, MDA, and MOR was 0.29, 0.37, 0.86, 1.09 and 7.56 ng/mL, respectively. The method was applied to municipal wastewater samples collected from sewage treatment plants in Kuantan, Pahang, in which AM, MA and MDA were detected in all 3 samples. Universiti Kebangsaan Malaysia 2023-02-22 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/37910/1/Izani_27_1_1.pdf Mohammad Azimuddin, Izani and Nurul `Azyyati, Sabri and Nurul Nadiah, Hamidon and Kooi, Yeong Khaw and Iekhsan, Othman and Mueller, Jochen and Tay, Joo Hui (2023) Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study. Malaysian Journal of Analytical Sciences, 27 (1). pp. 1-7. ISSN 1394-2506. (Published) https://mjas.analis.com.my/mjas/v27_n1/pdf/Izani_27_1_1.pdf https://mjas.analis.com.my/mjas/v27_n1/pdf/Izani_27_1_1.pdf |
| spellingShingle | QD Chemistry TD Environmental technology. Sanitary engineering TP Chemical technology Mohammad Azimuddin, Izani Nurul `Azyyati, Sabri Nurul Nadiah, Hamidon Kooi, Yeong Khaw Iekhsan, Othman Mueller, Jochen Tay, Joo Hui Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study |
| title | Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study |
| title_full | Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study |
| title_fullStr | Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study |
| title_full_unstemmed | Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study |
| title_short | Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study |
| title_sort | analysis of illicit drugs in municipal wastewater using lcms ms a method validation study |
| topic | QD Chemistry TD Environmental technology. Sanitary engineering TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/37910/1/Izani_27_1_1.pdf |
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