Ultrasensitive aptasensor using electrospun MXene/polyvinylidene fluoride nanofiber composite for Ochratoxin A detection
Ochratoxin A (OTA) has harmful effects to human and animal health; therefore, sensitive and selective detection of OTA is highly demanded. Herein, an ultrasensitive electrochemical aptasensor electrode comprising electrospun MXene/polyvinylidene fluoride (Ti3C2Tx/PVDF) nanofiber composite is present...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Elsevier
2022
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| Online Access: | http://umpir.ump.edu.my/id/eprint/38056/1/Food%20Chemistry.pdf http://umpir.ump.edu.my/id/eprint/38056/7/Ultrasensitive%20aptasensor%20using%20electrospun%20MXene.pdf |
| _version_ | 1825815043256614912 |
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| author | Al Dhahebi, Adel Mohammed Jose, Rajan Mazli, Mustapha Mohamed Shuaib, Mohamed Saheed |
| author_facet | Al Dhahebi, Adel Mohammed Jose, Rajan Mazli, Mustapha Mohamed Shuaib, Mohamed Saheed |
| author_sort | Al Dhahebi, Adel Mohammed |
| collection | UMP |
| description | Ochratoxin A (OTA) has harmful effects to human and animal health; therefore, sensitive and selective detection of OTA is highly demanded. Herein, an ultrasensitive electrochemical aptasensor electrode comprising electrospun MXene/polyvinylidene fluoride (Ti3C2Tx/PVDF) nanofiber composite is presented. Addition of Ti3C2Tx up to 13% effectively increased the fiber diameter and lowered the β-phase of PVDF nanofibers, consequently lowering the charge transfer resistance. The nanofiber composite is then coated on the screen-printed carbon electrode to chemically functionalized with saline and aldehyde groups for efficient aptamer loading. The optimized aptasensor demonstrated sensitive detection of OTA over the dynamic concentration range from 1 fg mL−1 to 1 ng mL−1 with a limit of detection of 2.15 fg mL−1 and quantification limit of 6.52 fg mL−1, with high selectivity. The aptasensor could detect the OTA at femtogram per milliliter concentration in grape juice samples, demonstrating its enormous potential for OTA detection in food industry. |
| first_indexed | 2024-03-06T13:07:34Z |
| format | Article |
| id | UMPir38056 |
| institution | Universiti Malaysia Pahang |
| language | English English |
| last_indexed | 2024-03-06T13:07:34Z |
| publishDate | 2022 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | UMPir380562023-08-02T02:54:13Z http://umpir.ump.edu.my/id/eprint/38056/ Ultrasensitive aptasensor using electrospun MXene/polyvinylidene fluoride nanofiber composite for Ochratoxin A detection Al Dhahebi, Adel Mohammed Jose, Rajan Mazli, Mustapha Mohamed Shuaib, Mohamed Saheed QD Chemistry Ochratoxin A (OTA) has harmful effects to human and animal health; therefore, sensitive and selective detection of OTA is highly demanded. Herein, an ultrasensitive electrochemical aptasensor electrode comprising electrospun MXene/polyvinylidene fluoride (Ti3C2Tx/PVDF) nanofiber composite is presented. Addition of Ti3C2Tx up to 13% effectively increased the fiber diameter and lowered the β-phase of PVDF nanofibers, consequently lowering the charge transfer resistance. The nanofiber composite is then coated on the screen-printed carbon electrode to chemically functionalized with saline and aldehyde groups for efficient aptamer loading. The optimized aptasensor demonstrated sensitive detection of OTA over the dynamic concentration range from 1 fg mL−1 to 1 ng mL−1 with a limit of detection of 2.15 fg mL−1 and quantification limit of 6.52 fg mL−1, with high selectivity. The aptasensor could detect the OTA at femtogram per milliliter concentration in grape juice samples, demonstrating its enormous potential for OTA detection in food industry. Elsevier 2022-10 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/38056/1/Food%20Chemistry.pdf pdf en http://umpir.ump.edu.my/id/eprint/38056/7/Ultrasensitive%20aptasensor%20using%20electrospun%20MXene.pdf Al Dhahebi, Adel Mohammed and Jose, Rajan and Mazli, Mustapha and Mohamed Shuaib, Mohamed Saheed (2022) Ultrasensitive aptasensor using electrospun MXene/polyvinylidene fluoride nanofiber composite for Ochratoxin A detection. Food Chemistry, 390 (133105). pp. 1-10. ISSN 03088146. (Published) https://doi.org/10.1016/j.foodchem.2022.133105 https://doi.org/10.1016/j.foodchem.2022.133105 |
| spellingShingle | QD Chemistry Al Dhahebi, Adel Mohammed Jose, Rajan Mazli, Mustapha Mohamed Shuaib, Mohamed Saheed Ultrasensitive aptasensor using electrospun MXene/polyvinylidene fluoride nanofiber composite for Ochratoxin A detection |
| title | Ultrasensitive aptasensor using electrospun MXene/polyvinylidene fluoride nanofiber composite for Ochratoxin A detection |
| title_full | Ultrasensitive aptasensor using electrospun MXene/polyvinylidene fluoride nanofiber composite for Ochratoxin A detection |
| title_fullStr | Ultrasensitive aptasensor using electrospun MXene/polyvinylidene fluoride nanofiber composite for Ochratoxin A detection |
| title_full_unstemmed | Ultrasensitive aptasensor using electrospun MXene/polyvinylidene fluoride nanofiber composite for Ochratoxin A detection |
| title_short | Ultrasensitive aptasensor using electrospun MXene/polyvinylidene fluoride nanofiber composite for Ochratoxin A detection |
| title_sort | ultrasensitive aptasensor using electrospun mxene polyvinylidene fluoride nanofiber composite for ochratoxin a detection |
| topic | QD Chemistry |
| url | http://umpir.ump.edu.my/id/eprint/38056/1/Food%20Chemistry.pdf http://umpir.ump.edu.my/id/eprint/38056/7/Ultrasensitive%20aptasensor%20using%20electrospun%20MXene.pdf |
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