Optimization of solvent systems for color fractionation of monascus pigment
Monascus fungi are unique among the various microorganisms that produce edible pigments, which consist of red, orange, and yellow. These pigments are typically present in a mixture. Hence, pigment separation is needed. This study aims to optimize solvent systems that can effectively fractionate the...
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| Format: | Article |
| Language: | English |
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Universiti Malaysia Pahang
2023
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| Online Access: | http://umpir.ump.edu.my/id/eprint/38658/1/Optimization%20of%20Solvent%20Systems%20for%20Color%20Fractionation.pdf |
| _version_ | 1825815152073637888 |
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| author | Nur Fathin Shamirah, Daud Farhan, Mohd Said M. A., Mokhtar |
| author_facet | Nur Fathin Shamirah, Daud Farhan, Mohd Said M. A., Mokhtar |
| author_sort | Nur Fathin Shamirah, Daud |
| collection | UMP |
| description | Monascus fungi are unique among the various microorganisms that produce edible pigments, which consist of red, orange, and yellow. These pigments are typically present in a mixture. Hence, pigment separation is needed. This study aims to optimize solvent systems that can effectively fractionate the pigments. The optimization was carried out using column chromatography and the data was analyzed using Central Composite Design (CCD). The interaction of the solvent systems ethyl acetate : formic acid : acetic acid : water was optimized. The optimal solvent system obtained was 98 : 14 : 12 : 28 (vol/vol.) for ethyl acetate : formic acid : acetic acid : water, respectively. The red and yellow pigment obtained were 2.42 mg and 0.93 mg, respectively. Based on the finding, 72.2% of the total Monascus pigments was red while remaining was yellow. |
| first_indexed | 2024-03-06T13:09:10Z |
| format | Article |
| id | UMPir38658 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T13:09:10Z |
| publishDate | 2023 |
| publisher | Universiti Malaysia Pahang |
| record_format | dspace |
| spelling | UMPir386582024-02-19T07:45:14Z http://umpir.ump.edu.my/id/eprint/38658/ Optimization of solvent systems for color fractionation of monascus pigment Nur Fathin Shamirah, Daud Farhan, Mohd Said M. A., Mokhtar TP Chemical technology Monascus fungi are unique among the various microorganisms that produce edible pigments, which consist of red, orange, and yellow. These pigments are typically present in a mixture. Hence, pigment separation is needed. This study aims to optimize solvent systems that can effectively fractionate the pigments. The optimization was carried out using column chromatography and the data was analyzed using Central Composite Design (CCD). The interaction of the solvent systems ethyl acetate : formic acid : acetic acid : water was optimized. The optimal solvent system obtained was 98 : 14 : 12 : 28 (vol/vol.) for ethyl acetate : formic acid : acetic acid : water, respectively. The red and yellow pigment obtained were 2.42 mg and 0.93 mg, respectively. Based on the finding, 72.2% of the total Monascus pigments was red while remaining was yellow. Universiti Malaysia Pahang 2023-12 Article PeerReviewed pdf en cc_by_nc_4 http://umpir.ump.edu.my/id/eprint/38658/1/Optimization%20of%20Solvent%20Systems%20for%20Color%20Fractionation.pdf Nur Fathin Shamirah, Daud and Farhan, Mohd Said and M. A., Mokhtar (2023) Optimization of solvent systems for color fractionation of monascus pigment. Journal of Chemical Engineering and Industrial Biotechnology (JCEIB), 9 (2). pp. 41-47. ISSN 0126-8139. (Published) https://doi.org/10.15282/jceib.v9i2.9642 https://doi.org/10.15282/jceib.v9i2.9642 |
| spellingShingle | TP Chemical technology Nur Fathin Shamirah, Daud Farhan, Mohd Said M. A., Mokhtar Optimization of solvent systems for color fractionation of monascus pigment |
| title | Optimization of solvent systems for color fractionation of monascus pigment |
| title_full | Optimization of solvent systems for color fractionation of monascus pigment |
| title_fullStr | Optimization of solvent systems for color fractionation of monascus pigment |
| title_full_unstemmed | Optimization of solvent systems for color fractionation of monascus pigment |
| title_short | Optimization of solvent systems for color fractionation of monascus pigment |
| title_sort | optimization of solvent systems for color fractionation of monascus pigment |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/38658/1/Optimization%20of%20Solvent%20Systems%20for%20Color%20Fractionation.pdf |
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