Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology
Recently, the widespread use of proteases in industries, such as detergent, food, and pharmaceutical sectors, has increased the demand for proteolytic enzymes in the global market. Although the main sources of commercial proteases are animals and microorganisms, the potential of using nonconventiona...
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| Format: | Conference or Workshop Item |
| Language: | English English |
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Malaysian Institute of Chemistry
2023
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| Online Access: | http://umpir.ump.edu.my/id/eprint/38988/1/Optimization%20of%20Protease%20Extraction%20from%20Ridged%20Gourd%20%28Luffa%20acutangula%29.pdf http://umpir.ump.edu.my/id/eprint/38988/2/Optimization%20of%20protease%20extraction%20from%20ridged%20gourd%20%28Luffa%20acutangula%29_ABS.pdf |
| _version_ | 1825815214446084096 |
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| author | Nur Fatin Nadia, Mohd Dzakir Erna Normaya, Abdullah Nur Amanina, Hassanuddin Syamimi Sulfiza, Shamsuri Anwar, Iqbal Mohd Bijarimi, Mat Piah Mohammad Norazmi, Ahmad |
| author_facet | Nur Fatin Nadia, Mohd Dzakir Erna Normaya, Abdullah Nur Amanina, Hassanuddin Syamimi Sulfiza, Shamsuri Anwar, Iqbal Mohd Bijarimi, Mat Piah Mohammad Norazmi, Ahmad |
| author_sort | Nur Fatin Nadia, Mohd Dzakir |
| collection | UMP |
| description | Recently, the widespread use of proteases in industries, such as detergent, food, and pharmaceutical sectors, has increased the demand for proteolytic enzymes in the global market. Although the main sources of commercial proteases are animals and microorganisms, the potential of using nonconventional sources, especially plants, should not be overlooked. In this study, proteolytic enzymes were extracted from the sarcocarp of ridged gourd (Luffa acutangula), and the effect of the extraction process on protease activity was evaluated. The crude enzyme was optimized via response surface methodology (RSM) using a central composite rotatable design (CCRD). Four independent variables were studied, namely, the pH, the concentrations of Triton X-100 (TX-100) and 2-mercaptoethanol, and the mixing time. The optimum level of each variable based on the RSM model was determined: at pH 6.38, 4.99% (v/v) TX-100, 0.15 M 2-mercaptoethanol, and a mixing time of 4.09 min, the optimum protease activity was estimated at 1.35 U/g. A verification test revealed satisfactory agreement between the model and experimental results with 96% desirability, indicating that the quadratic model generated from RSM is significant. |
| first_indexed | 2024-03-06T13:10:10Z |
| format | Conference or Workshop Item |
| id | UMPir38988 |
| institution | Universiti Malaysia Pahang |
| language | English English |
| last_indexed | 2024-03-06T13:10:10Z |
| publishDate | 2023 |
| publisher | Malaysian Institute of Chemistry |
| record_format | dspace |
| spelling | UMPir389882023-11-14T02:57:20Z http://umpir.ump.edu.my/id/eprint/38988/ Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology Nur Fatin Nadia, Mohd Dzakir Erna Normaya, Abdullah Nur Amanina, Hassanuddin Syamimi Sulfiza, Shamsuri Anwar, Iqbal Mohd Bijarimi, Mat Piah Mohammad Norazmi, Ahmad QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Recently, the widespread use of proteases in industries, such as detergent, food, and pharmaceutical sectors, has increased the demand for proteolytic enzymes in the global market. Although the main sources of commercial proteases are animals and microorganisms, the potential of using nonconventional sources, especially plants, should not be overlooked. In this study, proteolytic enzymes were extracted from the sarcocarp of ridged gourd (Luffa acutangula), and the effect of the extraction process on protease activity was evaluated. The crude enzyme was optimized via response surface methodology (RSM) using a central composite rotatable design (CCRD). Four independent variables were studied, namely, the pH, the concentrations of Triton X-100 (TX-100) and 2-mercaptoethanol, and the mixing time. The optimum level of each variable based on the RSM model was determined: at pH 6.38, 4.99% (v/v) TX-100, 0.15 M 2-mercaptoethanol, and a mixing time of 4.09 min, the optimum protease activity was estimated at 1.35 U/g. A verification test revealed satisfactory agreement between the model and experimental results with 96% desirability, indicating that the quadratic model generated from RSM is significant. Malaysian Institute of Chemistry 2023-06-29 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/38988/1/Optimization%20of%20Protease%20Extraction%20from%20Ridged%20Gourd%20%28Luffa%20acutangula%29.pdf pdf en http://umpir.ump.edu.my/id/eprint/38988/2/Optimization%20of%20protease%20extraction%20from%20ridged%20gourd%20%28Luffa%20acutangula%29_ABS.pdf Nur Fatin Nadia, Mohd Dzakir and Erna Normaya, Abdullah and Nur Amanina, Hassanuddin and Syamimi Sulfiza, Shamsuri and Anwar, Iqbal and Mohd Bijarimi, Mat Piah and Mohammad Norazmi, Ahmad (2023) Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology. In: 3rd IKMPB Online Symposium 2023 - Innovation in Chemistry Towards Sustainable Development , 29 June 2023 , Online. pp. 391-399., 25 (3). ISSN 1511-2292 (Published) https://doi.org/10.55373/MJCHEM.V25I3.391 |
| spellingShingle | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Nur Fatin Nadia, Mohd Dzakir Erna Normaya, Abdullah Nur Amanina, Hassanuddin Syamimi Sulfiza, Shamsuri Anwar, Iqbal Mohd Bijarimi, Mat Piah Mohammad Norazmi, Ahmad Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology |
| title | Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology |
| title_full | Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology |
| title_fullStr | Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology |
| title_full_unstemmed | Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology |
| title_short | Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology |
| title_sort | optimization of protease extraction from ridged gourd luffa acutangula sarcocarp via response surface methodology |
| topic | QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/38988/1/Optimization%20of%20Protease%20Extraction%20from%20Ridged%20Gourd%20%28Luffa%20acutangula%29.pdf http://umpir.ump.edu.my/id/eprint/38988/2/Optimization%20of%20protease%20extraction%20from%20ridged%20gourd%20%28Luffa%20acutangula%29_ABS.pdf |
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