Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations
In the current study, a novel high-performance liquid chromatographic method is being developed and validated to estimate benidipine hydrochloride in lipid-based pharmaceutical formulations including transfersome, ethosome, and transethosome. The chromatographic separation was accomplished on a ZORB...
| Main Authors: | , , , , |
|---|---|
| 格式: | 文件 |
| 语言: | English |
| 出版: |
Cosmos Scholars Publishing House
2022
|
| 主题: | |
| 在线阅读: | http://umpir.ump.edu.my/id/eprint/40283/1/Development%20and%20Validation%20of%20HPLC%20Method%20for%20Determination.pdf |
| _version_ | 1825815456631488512 |
|---|---|
| author | AL-Japairai, Khater Syed Mahmood, . Mohd Azmir, Arifin Nadiya, Abdul Halim Almurisi, Samah Hamed |
| author_facet | AL-Japairai, Khater Syed Mahmood, . Mohd Azmir, Arifin Nadiya, Abdul Halim Almurisi, Samah Hamed |
| author_sort | AL-Japairai, Khater |
| collection | UMP |
| description | In the current study, a novel high-performance liquid chromatographic method is being developed and validated to estimate benidipine hydrochloride in lipid-based pharmaceutical formulations including transfersome, ethosome, and transethosome. The chromatographic separation was accomplished on a ZORBAX Eclipse Plus C18 (4.6mm x 150mm) analytical column with a mobile phase consisting of a mixture of methanol and 50 mM phosphate buffer solution at a ratio of 70:30 (v/v). A standard calibration curve was used to quantitatively determine the medication at a UV wavelength of 237 nm. The limit of detection (LOD) and limit of quantitation (LOQ) were determined to be 0.005µg/ml and 0.015µg/ml, respectively. The relative standard deviation (%RSD) of the intra-day and inter-day studies for benidipine hydrochloride was less than 2%, and the percentage recovery of benidipine hydrochloride was found to be in the range of 98.57-100.27%. The method is specific, linear, accurate, precise, robust, and sensitive for its intended purpose, according to the results of the method validation. To determine the amount of benidipine hydrochloride and the effectiveness of drug entrapment in lipid-based formulated membrane like cell structures namely Transfersomes, Ethosomes, and Transethosomes the current method was successfully applied. |
| first_indexed | 2024-03-06T13:13:44Z |
| format | Article |
| id | UMPir40283 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T13:13:44Z |
| publishDate | 2022 |
| publisher | Cosmos Scholars Publishing House |
| record_format | dspace |
| spelling | UMPir402832024-02-07T03:42:27Z http://umpir.ump.edu.my/id/eprint/40283/ Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations AL-Japairai, Khater Syed Mahmood, . Mohd Azmir, Arifin Nadiya, Abdul Halim Almurisi, Samah Hamed TP Chemical technology In the current study, a novel high-performance liquid chromatographic method is being developed and validated to estimate benidipine hydrochloride in lipid-based pharmaceutical formulations including transfersome, ethosome, and transethosome. The chromatographic separation was accomplished on a ZORBAX Eclipse Plus C18 (4.6mm x 150mm) analytical column with a mobile phase consisting of a mixture of methanol and 50 mM phosphate buffer solution at a ratio of 70:30 (v/v). A standard calibration curve was used to quantitatively determine the medication at a UV wavelength of 237 nm. The limit of detection (LOD) and limit of quantitation (LOQ) were determined to be 0.005µg/ml and 0.015µg/ml, respectively. The relative standard deviation (%RSD) of the intra-day and inter-day studies for benidipine hydrochloride was less than 2%, and the percentage recovery of benidipine hydrochloride was found to be in the range of 98.57-100.27%. The method is specific, linear, accurate, precise, robust, and sensitive for its intended purpose, according to the results of the method validation. To determine the amount of benidipine hydrochloride and the effectiveness of drug entrapment in lipid-based formulated membrane like cell structures namely Transfersomes, Ethosomes, and Transethosomes the current method was successfully applied. Cosmos Scholars Publishing House 2022-10 Article PeerReviewed pdf en cc_by_nc http://umpir.ump.edu.my/id/eprint/40283/1/Development%20and%20Validation%20of%20HPLC%20Method%20for%20Determination.pdf AL-Japairai, Khater and Syed Mahmood, . and Mohd Azmir, Arifin and Nadiya, Abdul Halim and Almurisi, Samah Hamed (2022) Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations. International Journal of Membrane Science and Technology, 9 (2). pp. 40-47. ISSN 2410 -1869. (Published) https://doi.org/10.15379/ijmst.v9i2.1047 https://doi.org/10.15379/ijmst.v9i2.1047 |
| spellingShingle | TP Chemical technology AL-Japairai, Khater Syed Mahmood, . Mohd Azmir, Arifin Nadiya, Abdul Halim Almurisi, Samah Hamed Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations |
| title | Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations |
| title_full | Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations |
| title_fullStr | Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations |
| title_full_unstemmed | Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations |
| title_short | Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations |
| title_sort | development and validation of hplc method for determination of benidipine hydrochloride in lipid vesicles formulations |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/40283/1/Development%20and%20Validation%20of%20HPLC%20Method%20for%20Determination.pdf |
| work_keys_str_mv | AT aljapairaikhater developmentandvalidationofhplcmethodfordeterminationofbenidipinehydrochlorideinlipidvesiclesformulations AT syedmahmood developmentandvalidationofhplcmethodfordeterminationofbenidipinehydrochlorideinlipidvesiclesformulations AT mohdazmirarifin developmentandvalidationofhplcmethodfordeterminationofbenidipinehydrochlorideinlipidvesiclesformulations AT nadiyaabdulhalim developmentandvalidationofhplcmethodfordeterminationofbenidipinehydrochlorideinlipidvesiclesformulations AT almurisisamahhamed developmentandvalidationofhplcmethodfordeterminationofbenidipinehydrochlorideinlipidvesiclesformulations |