Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia
Decaffeination by microbial degradation is currently the most optimal and low-cost approach, involving only microbial cells and/or their enzymes. The bacterium was characterized using a series of biochemical tests. Positive results were obtained from carbohydrate fermentation, citrate utilization, a...
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| Format: | Article |
| Language: | English |
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DRPM Universitas Indonesia
2023
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| Online Access: | http://umpir.ump.edu.my/id/eprint/40400/1/Isolation%20and%20Characterization%20of%20Caffeine-Degrading%20Bacteria.pdf |
| _version_ | 1796996247539679232 |
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| author | Dullah, Elvina Clarie Mohd Fazli, Farida Asras |
| author_facet | Dullah, Elvina Clarie Mohd Fazli, Farida Asras |
| author_sort | Dullah, Elvina Clarie |
| collection | UMP |
| description | Decaffeination by microbial degradation is currently the most optimal and low-cost approach, involving only microbial cells and/or their enzymes. The bacterium was characterized using a series of biochemical tests. Positive results were obtained from carbohydrate fermentation, citrate utilization, and catalase tests, while negative results were obtained from Voges-Proskauer (VP) and indole tests. Three different caffeine concentrations of 0.25%, 0.4%, and 2% were tested and measured through Gas Chromatography-Mass Spectrophotometry (GC-MS) analysis. The highest caffeine reduction (89.25%) was found when 0.25% caffeine was used in the media. Only a small amount of caffeine was reduced to 0.4% and 2%, with 34.78% and 46.16%, respectively. Under microscopic observation, the shape of the isolated bacteria was rod bacilli and was stained with a pink color, which indicates Gram-negative bacteria. Comparing the results with previous research and observing the color of the pure culture, which revealed a yellow color, bacteria isolated from the coffee plantation area can be concluded to be a partially identified Pseudomonas sp. |
| first_indexed | 2024-03-06T13:13:55Z |
| format | Article |
| id | UMPir40400 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T13:13:55Z |
| publishDate | 2023 |
| publisher | DRPM Universitas Indonesia |
| record_format | dspace |
| spelling | UMPir404002024-02-16T07:59:13Z http://umpir.ump.edu.my/id/eprint/40400/ Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia Dullah, Elvina Clarie Mohd Fazli, Farida Asras Q Science (General) S Agriculture (General) Decaffeination by microbial degradation is currently the most optimal and low-cost approach, involving only microbial cells and/or their enzymes. The bacterium was characterized using a series of biochemical tests. Positive results were obtained from carbohydrate fermentation, citrate utilization, and catalase tests, while negative results were obtained from Voges-Proskauer (VP) and indole tests. Three different caffeine concentrations of 0.25%, 0.4%, and 2% were tested and measured through Gas Chromatography-Mass Spectrophotometry (GC-MS) analysis. The highest caffeine reduction (89.25%) was found when 0.25% caffeine was used in the media. Only a small amount of caffeine was reduced to 0.4% and 2%, with 34.78% and 46.16%, respectively. Under microscopic observation, the shape of the isolated bacteria was rod bacilli and was stained with a pink color, which indicates Gram-negative bacteria. Comparing the results with previous research and observing the color of the pure culture, which revealed a yellow color, bacteria isolated from the coffee plantation area can be concluded to be a partially identified Pseudomonas sp. DRPM Universitas Indonesia 2023 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/40400/1/Isolation%20and%20Characterization%20of%20Caffeine-Degrading%20Bacteria.pdf Dullah, Elvina Clarie and Mohd Fazli, Farida Asras (2023) Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia. Makara Journal of Technology, 27 (3). pp. 93-98. ISSN 2355-2786. (Published) https://doi.org/10.7454/mst.v27i3.1462 https://doi.org/10.7454/mst.v27i3.1462 |
| spellingShingle | Q Science (General) S Agriculture (General) Dullah, Elvina Clarie Mohd Fazli, Farida Asras Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia |
| title | Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia |
| title_full | Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia |
| title_fullStr | Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia |
| title_full_unstemmed | Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia |
| title_short | Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia |
| title_sort | isolation and characterization of caffeine degrading bacteria from coffee plantation areas in malaysia |
| topic | Q Science (General) S Agriculture (General) |
| url | http://umpir.ump.edu.my/id/eprint/40400/1/Isolation%20and%20Characterization%20of%20Caffeine-Degrading%20Bacteria.pdf |
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