Prevalence of antibiotic resistance in E. coli in leachate
Landfill is one of the common practices for the disposal of municipal solid waste and may include possible contaminants such as antibiotics and heavy metals. The leachate produced from landfill has the potential to become a reservoir of antibiotic residues and heavy metals, leading to the disseminat...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Elsevier Ltd
2023
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| Online Access: | http://umpir.ump.edu.my/id/eprint/40413/1/Prevalence%20of%20antibiotic%20resistance%20in%20E.%20coli%20in%20leachate.pdf http://umpir.ump.edu.my/id/eprint/40413/2/Prevalence%20of%20antibiotic%20resistance%20in%20E.%20coli%20in%20leachate_ABS.pdf |
| _version_ | 1796996250169507840 |
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| author | Nurul Syazana, Azman Nazira, Mahmud Hajar Fauzan, Ahmad Mohd Fazli, Farida Asras Noor Faizul Hadry, Nordin Nurul Shakila, Khalid Nurul Azyyati, Sabri |
| author_facet | Nurul Syazana, Azman Nazira, Mahmud Hajar Fauzan, Ahmad Mohd Fazli, Farida Asras Noor Faizul Hadry, Nordin Nurul Shakila, Khalid Nurul Azyyati, Sabri |
| author_sort | Nurul Syazana, Azman |
| collection | UMP |
| description | Landfill is one of the common practices for the disposal of municipal solid waste and may include possible contaminants such as antibiotics and heavy metals. The leachate produced from landfill has the potential to become a reservoir of antibiotic residues and heavy metals, leading to the dissemination of antibiotic resistance to the nearby environment. Not only that, enteric bacteria like Escherichia coli (E. coli) may be exposed to these contaminants. E. coli tended to survive in such stressful conditions due to the activation of the stress response. Therefore, this study was conducted to investigate the antibiotic susceptibility of E. coli in leachate. The E. coli was isolated in a landfill in Jabor, Kuantan, Pahang from three sampling points which were garbage hill, leachate pond, and river. The concentration of heavy metals was analyzed using ICP-MS while the antibiotic susceptibility test was performed using the disc diffusion method. Four out of six parameters measured have exceeded the standard permitted range where TSS was 110–580 mg/mL, BOD was 28–176 mg/mL, COD was 447–5100 mg/mL and NH3-N was 9–22 mg/mL. There were five elements (chromium, zinc, copper, manganese, and iron) were detected in leachate where the concentration of iron was the highest (0.158 ± 0.0203–2.287 ± 0.0706 mg/mL) among all metals measured. Besides, the colonies of E. coli were successfully isolated from each sampling point. In this study, all isolated E. coli from garbage hill and leachate pond show resistance towards three antibiotics tested (sulfamethoxazole, tetracycline, and erythromycin). However, isolated E. coli from the river shows intermediate sensitivity to sulfamethoxazole but is resistant to tetracycline and erythromycin. Based on the results, we can conclude that the landfill has the potential to retain pollutants such as TSS, COD, BOD, NH3-N, and antibiotic resistance even though the concentration of heavy metals decreased and meet the standard permitted limit. It shows that the landfill and leachates may act as an important reservoir of heavy metals and antibiotic resistance and potentially affect the environment. |
| first_indexed | 2024-04-22T01:26:10Z |
| format | Article |
| id | UMPir40413 |
| institution | Universiti Malaysia Pahang |
| language | English English |
| last_indexed | 2024-04-22T01:26:10Z |
| publishDate | 2023 |
| publisher | Elsevier Ltd |
| record_format | dspace |
| spelling | UMPir404132024-04-16T04:23:39Z http://umpir.ump.edu.my/id/eprint/40413/ Prevalence of antibiotic resistance in E. coli in leachate Nurul Syazana, Azman Nazira, Mahmud Hajar Fauzan, Ahmad Mohd Fazli, Farida Asras Noor Faizul Hadry, Nordin Nurul Shakila, Khalid Nurul Azyyati, Sabri HD Industries. Land use. Labor Q Science (General) T Technology (General) Landfill is one of the common practices for the disposal of municipal solid waste and may include possible contaminants such as antibiotics and heavy metals. The leachate produced from landfill has the potential to become a reservoir of antibiotic residues and heavy metals, leading to the dissemination of antibiotic resistance to the nearby environment. Not only that, enteric bacteria like Escherichia coli (E. coli) may be exposed to these contaminants. E. coli tended to survive in such stressful conditions due to the activation of the stress response. Therefore, this study was conducted to investigate the antibiotic susceptibility of E. coli in leachate. The E. coli was isolated in a landfill in Jabor, Kuantan, Pahang from three sampling points which were garbage hill, leachate pond, and river. The concentration of heavy metals was analyzed using ICP-MS while the antibiotic susceptibility test was performed using the disc diffusion method. Four out of six parameters measured have exceeded the standard permitted range where TSS was 110–580 mg/mL, BOD was 28–176 mg/mL, COD was 447–5100 mg/mL and NH3-N was 9–22 mg/mL. There were five elements (chromium, zinc, copper, manganese, and iron) were detected in leachate where the concentration of iron was the highest (0.158 ± 0.0203–2.287 ± 0.0706 mg/mL) among all metals measured. Besides, the colonies of E. coli were successfully isolated from each sampling point. In this study, all isolated E. coli from garbage hill and leachate pond show resistance towards three antibiotics tested (sulfamethoxazole, tetracycline, and erythromycin). However, isolated E. coli from the river shows intermediate sensitivity to sulfamethoxazole but is resistant to tetracycline and erythromycin. Based on the results, we can conclude that the landfill has the potential to retain pollutants such as TSS, COD, BOD, NH3-N, and antibiotic resistance even though the concentration of heavy metals decreased and meet the standard permitted limit. It shows that the landfill and leachates may act as an important reservoir of heavy metals and antibiotic resistance and potentially affect the environment. Elsevier Ltd 2023 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/40413/1/Prevalence%20of%20antibiotic%20resistance%20in%20E.%20coli%20in%20leachate.pdf pdf en http://umpir.ump.edu.my/id/eprint/40413/2/Prevalence%20of%20antibiotic%20resistance%20in%20E.%20coli%20in%20leachate_ABS.pdf Nurul Syazana, Azman and Nazira, Mahmud and Hajar Fauzan, Ahmad and Mohd Fazli, Farida Asras and Noor Faizul Hadry, Nordin and Nurul Shakila, Khalid and Nurul Azyyati, Sabri and UNSPECIFIED (2023) Prevalence of antibiotic resistance in E. coli in leachate. Materials Today: Proceedings. pp. 1-8. ISSN 2214-7853. (In Press / Online First) (In Press / Online First) https://doi.org/10.1016/j.matpr.2023.04.358 https://doi.org/10.1016/j.matpr.2023.04.358 |
| spellingShingle | HD Industries. Land use. Labor Q Science (General) T Technology (General) Nurul Syazana, Azman Nazira, Mahmud Hajar Fauzan, Ahmad Mohd Fazli, Farida Asras Noor Faizul Hadry, Nordin Nurul Shakila, Khalid Nurul Azyyati, Sabri Prevalence of antibiotic resistance in E. coli in leachate |
| title | Prevalence of antibiotic resistance in E. coli in leachate |
| title_full | Prevalence of antibiotic resistance in E. coli in leachate |
| title_fullStr | Prevalence of antibiotic resistance in E. coli in leachate |
| title_full_unstemmed | Prevalence of antibiotic resistance in E. coli in leachate |
| title_short | Prevalence of antibiotic resistance in E. coli in leachate |
| title_sort | prevalence of antibiotic resistance in e coli in leachate |
| topic | HD Industries. Land use. Labor Q Science (General) T Technology (General) |
| url | http://umpir.ump.edu.my/id/eprint/40413/1/Prevalence%20of%20antibiotic%20resistance%20in%20E.%20coli%20in%20leachate.pdf http://umpir.ump.edu.my/id/eprint/40413/2/Prevalence%20of%20antibiotic%20resistance%20in%20E.%20coli%20in%20leachate_ABS.pdf |
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