Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes
The burgeoning crises of antimicrobial resistance and plastic pollution are converging in healthcare settings, presenting a complex challenge to global health. This study investigates the microbial populations in healthcare waste to understand the extent of antimicrobial resistance and the potential...
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| Format: | Article |
| Language: | English English English |
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Elsevier
2025
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| Online Access: | http://umpir.ump.edu.my/id/eprint/42937/1/1-s2.0-S0269749124020475-main.pdf http://umpir.ump.edu.my/id/eprint/42937/7/Shallow%20shotgun%20sequencing%20of%20healthcare%20waste%20reveals%20plastic-eating%20bacteria_ABST.pdf http://umpir.ump.edu.my/id/eprint/42937/8/Shallow%20shotgun%20sequencing%20of%20healthcare%20waste%20reveals%20plastic-eating%20bacteria.pdf |
| _version_ | 1825815970256519168 |
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| author | Siew, Shing Wei Mohamad Hazwan Fikri, Khairi Norhisham, Abdul Hamid Mohd Fazli, Farida Asras Hajar Fauzan, Ahmad |
| author_facet | Siew, Shing Wei Mohamad Hazwan Fikri, Khairi Norhisham, Abdul Hamid Mohd Fazli, Farida Asras Hajar Fauzan, Ahmad |
| author_sort | Siew, Shing Wei |
| collection | UMP |
| description | The burgeoning crises of antimicrobial resistance and plastic pollution are converging in healthcare settings, presenting a complex challenge to global health. This study investigates the microbial populations in healthcare waste to understand the extent of antimicrobial resistance and the potential for plastic degradation by bacteria. Our metagenomic analysis, using both amplicon and shallow shotgun sequencing, provided a comprehensive view of the taxonomic diversity and functional capacity of the microbial consortia. The viable bacteria in healthcare waste samples were analyzed employing full-length 16S rRNA sequencing, revealing a diverse bacterial community dominated by Firmicutes and Proteobacteria phyla. Notably, Proteus mirabilis VFC3/3 and Pseudomonas sp. VFA2/3 were detected, while Stenotrophomonas maltophilia VFV3/2 surfaced as the predominant species, holding implications for the spread of hospital-acquired infections and antimicrobial resistance. Antibiotic susceptibility testing identified multidrug-resistant strains conferring antimicrobial genes, including the broad-spectrum antibiotic carbapenem, underscoring the critical need for improved waste management and infection control measures. Remarkably, we found genes linked to the breakdown of plastic that encoded for enzymes of the esterase, depolymerase, and oxidoreductase classes. This suggests that specific bacteria found in medical waste may be able to reduce the amount of plastic pollution that comes from biological and medical waste. The information is helpful in formulating strategies to counter the combined problems of environmental pollution and antibiotic resistance. This study emphasises the importance of monitoring microbial communities in hospital waste in order to influence waste management procedures and public health policy. The findings highlight the need for a multidisciplinary approach to mitigate the risks associated with antimicrobial resistance and plastic waste, especially in hospital settings where they intersect most acutely. |
| first_indexed | 2024-12-09T02:30:57Z |
| format | Article |
| id | UMPir42937 |
| institution | Universiti Malaysia Pahang |
| language | English English English |
| last_indexed | 2025-03-06T04:03:17Z |
| publishDate | 2025 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | UMPir429372025-03-04T08:00:36Z http://umpir.ump.edu.my/id/eprint/42937/ Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes Siew, Shing Wei Mohamad Hazwan Fikri, Khairi Norhisham, Abdul Hamid Mohd Fazli, Farida Asras Hajar Fauzan, Ahmad Q Science (General) TP Chemical technology The burgeoning crises of antimicrobial resistance and plastic pollution are converging in healthcare settings, presenting a complex challenge to global health. This study investigates the microbial populations in healthcare waste to understand the extent of antimicrobial resistance and the potential for plastic degradation by bacteria. Our metagenomic analysis, using both amplicon and shallow shotgun sequencing, provided a comprehensive view of the taxonomic diversity and functional capacity of the microbial consortia. The viable bacteria in healthcare waste samples were analyzed employing full-length 16S rRNA sequencing, revealing a diverse bacterial community dominated by Firmicutes and Proteobacteria phyla. Notably, Proteus mirabilis VFC3/3 and Pseudomonas sp. VFA2/3 were detected, while Stenotrophomonas maltophilia VFV3/2 surfaced as the predominant species, holding implications for the spread of hospital-acquired infections and antimicrobial resistance. Antibiotic susceptibility testing identified multidrug-resistant strains conferring antimicrobial genes, including the broad-spectrum antibiotic carbapenem, underscoring the critical need for improved waste management and infection control measures. Remarkably, we found genes linked to the breakdown of plastic that encoded for enzymes of the esterase, depolymerase, and oxidoreductase classes. This suggests that specific bacteria found in medical waste may be able to reduce the amount of plastic pollution that comes from biological and medical waste. The information is helpful in formulating strategies to counter the combined problems of environmental pollution and antibiotic resistance. This study emphasises the importance of monitoring microbial communities in hospital waste in order to influence waste management procedures and public health policy. The findings highlight the need for a multidisciplinary approach to mitigate the risks associated with antimicrobial resistance and plastic waste, especially in hospital settings where they intersect most acutely. Elsevier 2025-01-01 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/42937/1/1-s2.0-S0269749124020475-main.pdf pdf en http://umpir.ump.edu.my/id/eprint/42937/7/Shallow%20shotgun%20sequencing%20of%20healthcare%20waste%20reveals%20plastic-eating%20bacteria_ABST.pdf pdf en http://umpir.ump.edu.my/id/eprint/42937/8/Shallow%20shotgun%20sequencing%20of%20healthcare%20waste%20reveals%20plastic-eating%20bacteria.pdf Siew, Shing Wei and Mohamad Hazwan Fikri, Khairi and Norhisham, Abdul Hamid and Mohd Fazli, Farida Asras and Hajar Fauzan, Ahmad (2025) Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes. Environmental Pollution, 364 (125330). pp. 1-11. ISSN 1873-6424. (Published) https://doi.org/10.1016/j.envpol.2024.125330 https://doi.org/10.1016/j.envpol.2024.125330 |
| spellingShingle | Q Science (General) TP Chemical technology Siew, Shing Wei Mohamad Hazwan Fikri, Khairi Norhisham, Abdul Hamid Mohd Fazli, Farida Asras Hajar Fauzan, Ahmad Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes |
| title | Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes |
| title_full | Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes |
| title_fullStr | Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes |
| title_full_unstemmed | Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes |
| title_short | Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes |
| title_sort | shallow shotgun sequencing of healthcare waste reveals plastic eating bacteria with broad spectrum antibiotic resistance genes |
| topic | Q Science (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/42937/1/1-s2.0-S0269749124020475-main.pdf http://umpir.ump.edu.my/id/eprint/42937/7/Shallow%20shotgun%20sequencing%20of%20healthcare%20waste%20reveals%20plastic-eating%20bacteria_ABST.pdf http://umpir.ump.edu.my/id/eprint/42937/8/Shallow%20shotgun%20sequencing%20of%20healthcare%20waste%20reveals%20plastic-eating%20bacteria.pdf |
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