Analytical quantification method of protein denaturation in spray drying

Protein is the most valuable constituent of bovine milk, due to its high nutritional quality,unique physicochemical and functional properties,which were exploited to manufacture diverse range of dairy products.This research is to identify the protein denaturation by heat in lab scale spray dryer. Th...

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Main Author: Suria, Mahmud
Format: Undergraduates Project Papers
Language:English
Published: 2012
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/4413/1/06.Analytical%20quantification%20method%20of%20protein%20denaturation%20in%20spray%20drying.pdf
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author Suria, Mahmud
author_facet Suria, Mahmud
author_sort Suria, Mahmud
collection UMP
description Protein is the most valuable constituent of bovine milk, due to its high nutritional quality,unique physicochemical and functional properties,which were exploited to manufacture diverse range of dairy products.This research is to identify the protein denaturation by heat in lab scale spray dryer. The Lab-Plant SD06AG Laboratory Scale Spray Dryer is used to produce protein powder from a pure whey protein powder.This work is to establish a method for preserving protein content in whey protein during the spray drying which is by employing the microencapsulation technique and also by controlling the spray drying operating parameters.Protein denaturation was reduced by adding of microencapsulation by using Maltodextrin.The moisture content analysis was determined via MS-07 Moisture Content Analyzer.Then,the morphology and particle size distribution of milk powders are determined by using Malvern Mastersizer.The quality of protein that undergo spray drying process which is before and after it analyzed by using high-performance liquid chromatography (HPLC).The analysis suggested a severe protein denaturation (60% and 40%) at higher spray drying temperature (180 °C and 190°C) but can be reduced up to 29% and 20% when microencapsulation technique is employed.
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spelling UMPir44132023-04-10T03:33:39Z http://umpir.ump.edu.my/id/eprint/4413/ Analytical quantification method of protein denaturation in spray drying Suria, Mahmud TP Chemical technology Protein is the most valuable constituent of bovine milk, due to its high nutritional quality,unique physicochemical and functional properties,which were exploited to manufacture diverse range of dairy products.This research is to identify the protein denaturation by heat in lab scale spray dryer. The Lab-Plant SD06AG Laboratory Scale Spray Dryer is used to produce protein powder from a pure whey protein powder.This work is to establish a method for preserving protein content in whey protein during the spray drying which is by employing the microencapsulation technique and also by controlling the spray drying operating parameters.Protein denaturation was reduced by adding of microencapsulation by using Maltodextrin.The moisture content analysis was determined via MS-07 Moisture Content Analyzer.Then,the morphology and particle size distribution of milk powders are determined by using Malvern Mastersizer.The quality of protein that undergo spray drying process which is before and after it analyzed by using high-performance liquid chromatography (HPLC).The analysis suggested a severe protein denaturation (60% and 40%) at higher spray drying temperature (180 °C and 190°C) but can be reduced up to 29% and 20% when microencapsulation technique is employed. 2012-01 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/4413/1/06.Analytical%20quantification%20method%20of%20protein%20denaturation%20in%20spray%20drying.pdf Suria, Mahmud (2012) Analytical quantification method of protein denaturation in spray drying. Faculty of Chemical & Natural Resource Engineering, Universiti Malaysia Pahang.
spellingShingle TP Chemical technology
Suria, Mahmud
Analytical quantification method of protein denaturation in spray drying
title Analytical quantification method of protein denaturation in spray drying
title_full Analytical quantification method of protein denaturation in spray drying
title_fullStr Analytical quantification method of protein denaturation in spray drying
title_full_unstemmed Analytical quantification method of protein denaturation in spray drying
title_short Analytical quantification method of protein denaturation in spray drying
title_sort analytical quantification method of protein denaturation in spray drying
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/4413/1/06.Analytical%20quantification%20method%20of%20protein%20denaturation%20in%20spray%20drying.pdf
work_keys_str_mv AT suriamahmud analyticalquantificationmethodofproteindenaturationinspraydrying