3-Bromo-1-Hydroxy-9,10-Anthraquinone (BHAQ) Inhibits Growth and Migration of the Human Breast Cancer Cell Lines MCF-7 and MDA-MB231
Breast cancer is becoming more prominent in women today. As of now, there are no effective treatments in treating metastatic breast cancer. We have tested the cytotoxic and anti-migration effects of BHAQ, a synthesized anthraquinone, on two breast cancer cell lines, MCF-7 and MDA-MB231. Anthraquinon...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI - Open Access Publishing
2013
|
| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/5212/1/fist-2013-nadeem-art3-Bromo-1-Hydroxy.pdf |
| _version_ | 1825821627567308800 |
|---|---|
| author | Akhtar, Muhammad Nadeem Nadiah, Abu Wan, Yong Ho Swee, Keong Yeap Noorjahan Banu, Alitheen |
| author_facet | Akhtar, Muhammad Nadeem Nadiah, Abu Wan, Yong Ho Swee, Keong Yeap Noorjahan Banu, Alitheen |
| author_sort | Akhtar, Muhammad Nadeem |
| collection | UMP |
| description | Breast cancer is becoming more prominent in women today. As of now, there are no effective treatments in treating metastatic breast cancer. We have tested the cytotoxic and anti-migration effects of BHAQ, a synthesized anthraquinone, on two breast cancer cell lines, MCF-7 and MDA-MB231. Anthraquinones are an interesting class of molecules that display a wide spectrum of biological applications, including anticancer properties. Cellular inhibition was tested through a MTT assay, double acridine orange/propidium iodide staining and FACS cell cycle analysis. Inhibition of migration was tested by the wound healing method, and migration through a Boyden chamber. BHAQ was cytotoxic towards both cell lines in a dose dependent and possibly cell-dependent manner. Additionally, BHAQ also inhibited the migration of the highly metastatic MDA-MB231 cell line. |
| first_indexed | 2024-03-06T11:44:24Z |
| format | Article |
| id | UMPir5212 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T11:44:24Z |
| publishDate | 2013 |
| publisher | MDPI - Open Access Publishing |
| record_format | dspace |
| spelling | UMPir52122018-04-18T04:15:05Z http://umpir.ump.edu.my/id/eprint/5212/ 3-Bromo-1-Hydroxy-9,10-Anthraquinone (BHAQ) Inhibits Growth and Migration of the Human Breast Cancer Cell Lines MCF-7 and MDA-MB231 Akhtar, Muhammad Nadeem Nadiah, Abu Wan, Yong Ho Swee, Keong Yeap Noorjahan Banu, Alitheen QD Chemistry Breast cancer is becoming more prominent in women today. As of now, there are no effective treatments in treating metastatic breast cancer. We have tested the cytotoxic and anti-migration effects of BHAQ, a synthesized anthraquinone, on two breast cancer cell lines, MCF-7 and MDA-MB231. Anthraquinones are an interesting class of molecules that display a wide spectrum of biological applications, including anticancer properties. Cellular inhibition was tested through a MTT assay, double acridine orange/propidium iodide staining and FACS cell cycle analysis. Inhibition of migration was tested by the wound healing method, and migration through a Boyden chamber. BHAQ was cytotoxic towards both cell lines in a dose dependent and possibly cell-dependent manner. Additionally, BHAQ also inhibited the migration of the highly metastatic MDA-MB231 cell line. MDPI - Open Access Publishing 2013 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5212/1/fist-2013-nadeem-art3-Bromo-1-Hydroxy.pdf Akhtar, Muhammad Nadeem and Nadiah, Abu and Wan, Yong Ho and Swee, Keong Yeap and Noorjahan Banu, Alitheen (2013) 3-Bromo-1-Hydroxy-9,10-Anthraquinone (BHAQ) Inhibits Growth and Migration of the Human Breast Cancer Cell Lines MCF-7 and MDA-MB231. Molecules, 18 (9). pp. 10367-10377. ISSN 1420-3049 (print); 1420-3049 (online). (Published) http://dx.doi.org/10.3390/molecules180910367 DOI: 10.3390/molecules180910367 |
| spellingShingle | QD Chemistry Akhtar, Muhammad Nadeem Nadiah, Abu Wan, Yong Ho Swee, Keong Yeap Noorjahan Banu, Alitheen 3-Bromo-1-Hydroxy-9,10-Anthraquinone (BHAQ) Inhibits Growth and Migration of the Human Breast Cancer Cell Lines MCF-7 and MDA-MB231 |
| title | 3-Bromo-1-Hydroxy-9,10-Anthraquinone (BHAQ) Inhibits Growth and Migration of the Human Breast Cancer Cell Lines MCF-7 and MDA-MB231 |
| title_full | 3-Bromo-1-Hydroxy-9,10-Anthraquinone (BHAQ) Inhibits Growth and Migration of the Human Breast Cancer Cell Lines MCF-7 and MDA-MB231 |
| title_fullStr | 3-Bromo-1-Hydroxy-9,10-Anthraquinone (BHAQ) Inhibits Growth and Migration of the Human Breast Cancer Cell Lines MCF-7 and MDA-MB231 |
| title_full_unstemmed | 3-Bromo-1-Hydroxy-9,10-Anthraquinone (BHAQ) Inhibits Growth and Migration of the Human Breast Cancer Cell Lines MCF-7 and MDA-MB231 |
| title_short | 3-Bromo-1-Hydroxy-9,10-Anthraquinone (BHAQ) Inhibits Growth and Migration of the Human Breast Cancer Cell Lines MCF-7 and MDA-MB231 |
| title_sort | 3 bromo 1 hydroxy 9 10 anthraquinone bhaq inhibits growth and migration of the human breast cancer cell lines mcf 7 and mda mb231 |
| topic | QD Chemistry |
| url | http://umpir.ump.edu.my/id/eprint/5212/1/fist-2013-nadeem-art3-Bromo-1-Hydroxy.pdf |
| work_keys_str_mv | AT akhtarmuhammadnadeem 3bromo1hydroxy910anthraquinonebhaqinhibitsgrowthandmigrationofthehumanbreastcancercelllinesmcf7andmdamb231 AT nadiahabu 3bromo1hydroxy910anthraquinonebhaqinhibitsgrowthandmigrationofthehumanbreastcancercelllinesmcf7andmdamb231 AT wanyongho 3bromo1hydroxy910anthraquinonebhaqinhibitsgrowthandmigrationofthehumanbreastcancercelllinesmcf7andmdamb231 AT sweekeongyeap 3bromo1hydroxy910anthraquinonebhaqinhibitsgrowthandmigrationofthehumanbreastcancercelllinesmcf7andmdamb231 AT noorjahanbanualitheen 3bromo1hydroxy910anthraquinonebhaqinhibitsgrowthandmigrationofthehumanbreastcancercelllinesmcf7andmdamb231 |