Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters

Superovulation has become a common assisted reproductive technology in the field of animal reproduction. In addition, zona-free hamster oocytes have been used in heterologous in vitro fertilization research to evaluate sperm function. A study was conducted to compare eight different superovulation p...

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Main Authors: Jaya Vejayan, Palliah, Sarsaifi, Kazhal, Omar, Mohamed-Ariff, Rosnina, H. Yusoff, Abd-Wahid, Haron, Hani, Homayoun, Yimer, Nurhusein, Naing, Soe W., Abas Mazni, Othman
Format: Article
Language:English
Published: Science Alert 2013
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Online Access:http://umpir.ump.edu.my/id/eprint/5240/1/journal.pdf
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author Jaya Vejayan, Palliah
Sarsaifi, Kazhal
Omar, Mohamed-Ariff
Rosnina, H. Yusoff
Abd-Wahid, Haron
Hani, Homayoun
Yimer, Nurhusein
Naing, Soe W.
Abas Mazni, Othman
author_facet Jaya Vejayan, Palliah
Sarsaifi, Kazhal
Omar, Mohamed-Ariff
Rosnina, H. Yusoff
Abd-Wahid, Haron
Hani, Homayoun
Yimer, Nurhusein
Naing, Soe W.
Abas Mazni, Othman
author_sort Jaya Vejayan, Palliah
collection UMP
description Superovulation has become a common assisted reproductive technology in the field of animal reproduction. In addition, zona-free hamster oocytes have been used in heterologous in vitro fertilization research to evaluate sperm function. A study was conducted to compare eight different superovulation protocols for golden hamsters using two concentrations of human Chorionic Gonadotropin (hCG) given at two time intervals post-pregnant mare’s serum gonadotrophin (PMSG) injection and two time intervals of oocyte harvesting. Fifty-six female golden hamsters were randomly and equally assigned into eight superovulation groups. Hamsters were superovulated initially with PMSG followed by human Chorionic Gonadotrophin (hCG). All the groups received 40 IU PMSG, either 40 or 45 IU hCG given at either 48-50 or 55-57 h post PMSG injection and the oocytes recovered at either 12-15 or 16-18 h after hCG injection. Higher number of recovered oocytes (51.57±0.83) and maturation rates (94.20%) (p<0.05) were detected in hamsters which received 45 IU hCG at 55-57 h after PMSG injection when the oocytes were recovered later at 16-18 h compared with hamsters in the other groups. Mean fertilization rate of hamsters given 45 IU hCG at 55-57 h post PMSG injection ranged from 77.89-78.84% and were significantly higher (p<0.05) than those that received hCG at 48-50 h post PMSG injection. In conclusion administration of 40 IU PMSG followed by 45 IU hCG injection at 55 and 57 h post PMSG injection followed by oocyte recovery after 16-18 h gave the highest response in oocyte recovery and maturation in golden hamsters.
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spelling UMPir52402018-02-21T06:57:53Z http://umpir.ump.edu.my/id/eprint/5240/ Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters Jaya Vejayan, Palliah Sarsaifi, Kazhal Omar, Mohamed-Ariff Rosnina, H. Yusoff Abd-Wahid, Haron Hani, Homayoun Yimer, Nurhusein Naing, Soe W. Abas Mazni, Othman Q Science (General) QR Microbiology Superovulation has become a common assisted reproductive technology in the field of animal reproduction. In addition, zona-free hamster oocytes have been used in heterologous in vitro fertilization research to evaluate sperm function. A study was conducted to compare eight different superovulation protocols for golden hamsters using two concentrations of human Chorionic Gonadotropin (hCG) given at two time intervals post-pregnant mare’s serum gonadotrophin (PMSG) injection and two time intervals of oocyte harvesting. Fifty-six female golden hamsters were randomly and equally assigned into eight superovulation groups. Hamsters were superovulated initially with PMSG followed by human Chorionic Gonadotrophin (hCG). All the groups received 40 IU PMSG, either 40 or 45 IU hCG given at either 48-50 or 55-57 h post PMSG injection and the oocytes recovered at either 12-15 or 16-18 h after hCG injection. Higher number of recovered oocytes (51.57±0.83) and maturation rates (94.20%) (p<0.05) were detected in hamsters which received 45 IU hCG at 55-57 h after PMSG injection when the oocytes were recovered later at 16-18 h compared with hamsters in the other groups. Mean fertilization rate of hamsters given 45 IU hCG at 55-57 h post PMSG injection ranged from 77.89-78.84% and were significantly higher (p<0.05) than those that received hCG at 48-50 h post PMSG injection. In conclusion administration of 40 IU PMSG followed by 45 IU hCG injection at 55 and 57 h post PMSG injection followed by oocyte recovery after 16-18 h gave the highest response in oocyte recovery and maturation in golden hamsters. Science Alert 2013 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5240/1/journal.pdf Jaya Vejayan, Palliah and Sarsaifi, Kazhal and Omar, Mohamed-Ariff and Rosnina, H. Yusoff and Abd-Wahid, Haron and Hani, Homayoun and Yimer, Nurhusein and Naing, Soe W. and Abas Mazni, Othman (2013) Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters. Asian Journal of Animal and Veterinary Advances, 8 (6). pp. 796-805. ISSN 1683-9919 (print); 1996-3289 (online). (Published) http://dx.doi.org/10.3923/ajava.2013.796.805 DOI: 10.3923/ajava.2013.796.805
spellingShingle Q Science (General)
QR Microbiology
Jaya Vejayan, Palliah
Sarsaifi, Kazhal
Omar, Mohamed-Ariff
Rosnina, H. Yusoff
Abd-Wahid, Haron
Hani, Homayoun
Yimer, Nurhusein
Naing, Soe W.
Abas Mazni, Othman
Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters
title Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters
title_full Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters
title_fullStr Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters
title_full_unstemmed Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters
title_short Development of Superovulation Program and Heterologous in vitro Fertilization Test Assessment in Hamsters
title_sort development of superovulation program and heterologous in vitro fertilization test assessment in hamsters
topic Q Science (General)
QR Microbiology
url http://umpir.ump.edu.my/id/eprint/5240/1/journal.pdf
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