Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus

Urate oxidase or uricase is an enzyme that catalyses the oxidation of uric acid to allantoin and plays important rule in purine metabolism. The first important application discovered for uricase was in clinical biochemistry as diagnosis reagent for measurement of uric acid in blood. The precipita...

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Main Authors: Siti Hatijah, Mortan, Wan Ruhayu, Wan Ramli
Format: Article
Language:English
Published: International Academy Publishing (IAP) 2013
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/5549/1/267-S30006.pdf
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author Siti Hatijah, Mortan
Wan Ruhayu, Wan Ramli
author_facet Siti Hatijah, Mortan
Wan Ruhayu, Wan Ramli
author_sort Siti Hatijah, Mortan
collection UMP
description Urate oxidase or uricase is an enzyme that catalyses the oxidation of uric acid to allantoin and plays important rule in purine metabolism. The first important application discovered for uricase was in clinical biochemistry as diagnosis reagent for measurement of uric acid in blood. The precipitation of uric acid can leading to gout symptom. The main purpose of this research is to optimize the culture condition for maximum uricase production by Aspergillus flavus. The parameters studied were pH (ranged pH4 to pH8),the sucrose concentration (10g/l to 50 g/l) and the agitation rate(100 to 300 rpm). The Aspergillus flavus were inoculated in yeast extract with sucrose and incubated for 24 hours at 200 rpm and 30oC. The maximum enzyme activity obtained from the experiment is 0.03974 U/ml at pH 6, 200 rpm and 30 g/l sucrose concentration.
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spelling UMPir55492018-05-15T06:45:48Z http://umpir.ump.edu.my/id/eprint/5549/ Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus Siti Hatijah, Mortan Wan Ruhayu, Wan Ramli TP Chemical technology Urate oxidase or uricase is an enzyme that catalyses the oxidation of uric acid to allantoin and plays important rule in purine metabolism. The first important application discovered for uricase was in clinical biochemistry as diagnosis reagent for measurement of uric acid in blood. The precipitation of uric acid can leading to gout symptom. The main purpose of this research is to optimize the culture condition for maximum uricase production by Aspergillus flavus. The parameters studied were pH (ranged pH4 to pH8),the sucrose concentration (10g/l to 50 g/l) and the agitation rate(100 to 300 rpm). The Aspergillus flavus were inoculated in yeast extract with sucrose and incubated for 24 hours at 200 rpm and 30oC. The maximum enzyme activity obtained from the experiment is 0.03974 U/ml at pH 6, 200 rpm and 30 g/l sucrose concentration. International Academy Publishing (IAP) 2013 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5549/1/267-S30006.pdf Siti Hatijah, Mortan and Wan Ruhayu, Wan Ramli (2013) Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus. International Journal of Bioscience, Biochemistry and Bioinformatics, 3 (5). pp. 513-515. ISSN 2010-3638. (Published) http://dx.doi.org/10.7763/IJBBB.2013.V3.267 DOI: 10.7763/IJBBB.2013.V3.267
spellingShingle TP Chemical technology
Siti Hatijah, Mortan
Wan Ruhayu, Wan Ramli
Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus
title Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus
title_full Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus
title_fullStr Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus
title_full_unstemmed Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus
title_short Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus
title_sort preliminary study on factors that enhanced the production of uricase by aspergillus flavus
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/5549/1/267-S30006.pdf
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