Stability of papain in aqueous organic solvent by reverse phase liquid chromatography

Papain is one of the proteolytic enzymes found in the latex of the leaves and of the green fruit of the papaya tree (Carica papaya L.).It’s known to be highly used in the several industry like food,medical,brewing and many more. Hence,the objective of this research is to study the stability of papai...

Full description

Bibliographic Details
Main Author: Muhammad Yunus, Zainal Abidin
Format: Undergraduates Project Papers
Language:English
Published: 2013
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/5850/1/CD7067.pdf
_version_ 1825821696102236160
author Muhammad Yunus, Zainal Abidin
author_facet Muhammad Yunus, Zainal Abidin
author_sort Muhammad Yunus, Zainal Abidin
collection UMP
description Papain is one of the proteolytic enzymes found in the latex of the leaves and of the green fruit of the papaya tree (Carica papaya L.).It’s known to be highly used in the several industry like food,medical,brewing and many more. Hence,the objective of this research is to study the stability of papain in aqueous organic solvent and secondly to purify the papain using the AmberliteTM XAD7HP as the reverse phase liquid chromatography adsorbent.The research is based on experimental lab research.The effects of different concentrations of the water-miscible organic solvents acetonitrile and ethanol on the stability of papain in aqueous solution were studied.Batch adsorption using AmberliteTM XAD7HP was carried out to purify papain from papaya juice.The purified papain was analysed using the Bradford assay,the proteolytic papain activity assay and SDS PAGE.As for conclusion the of the study.The result obtained proved that papain has exhibited higher stability in aqueous acetonitrile than in ethanol under small margin.Decreases in the activity of the enzyme were observed at organic solvent concentrations above 60%.Papain with high purity was achieved by adsorption using the AmberliteTM XAD7HP.
first_indexed 2024-03-06T11:45:25Z
format Undergraduates Project Papers
id UMPir5850
institution Universiti Malaysia Pahang
language English
last_indexed 2024-03-06T11:45:25Z
publishDate 2013
record_format dspace
spelling UMPir58502021-06-09T03:00:40Z http://umpir.ump.edu.my/id/eprint/5850/ Stability of papain in aqueous organic solvent by reverse phase liquid chromatography Muhammad Yunus, Zainal Abidin QP Physiology Papain is one of the proteolytic enzymes found in the latex of the leaves and of the green fruit of the papaya tree (Carica papaya L.).It’s known to be highly used in the several industry like food,medical,brewing and many more. Hence,the objective of this research is to study the stability of papain in aqueous organic solvent and secondly to purify the papain using the AmberliteTM XAD7HP as the reverse phase liquid chromatography adsorbent.The research is based on experimental lab research.The effects of different concentrations of the water-miscible organic solvents acetonitrile and ethanol on the stability of papain in aqueous solution were studied.Batch adsorption using AmberliteTM XAD7HP was carried out to purify papain from papaya juice.The purified papain was analysed using the Bradford assay,the proteolytic papain activity assay and SDS PAGE.As for conclusion the of the study.The result obtained proved that papain has exhibited higher stability in aqueous acetonitrile than in ethanol under small margin.Decreases in the activity of the enzyme were observed at organic solvent concentrations above 60%.Papain with high purity was achieved by adsorption using the AmberliteTM XAD7HP. 2013-02 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5850/1/CD7067.pdf Muhammad Yunus, Zainal Abidin (2013) Stability of papain in aqueous organic solvent by reverse phase liquid chromatography. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
spellingShingle QP Physiology
Muhammad Yunus, Zainal Abidin
Stability of papain in aqueous organic solvent by reverse phase liquid chromatography
title Stability of papain in aqueous organic solvent by reverse phase liquid chromatography
title_full Stability of papain in aqueous organic solvent by reverse phase liquid chromatography
title_fullStr Stability of papain in aqueous organic solvent by reverse phase liquid chromatography
title_full_unstemmed Stability of papain in aqueous organic solvent by reverse phase liquid chromatography
title_short Stability of papain in aqueous organic solvent by reverse phase liquid chromatography
title_sort stability of papain in aqueous organic solvent by reverse phase liquid chromatography
topic QP Physiology
url http://umpir.ump.edu.my/id/eprint/5850/1/CD7067.pdf
work_keys_str_mv AT muhammadyunuszainalabidin stabilityofpapaininaqueousorganicsolventbyreversephaseliquidchromatography