Biohydrogen Production from Palm Oil Mill Effluent Using Immobilized Clostridium Butyricum EB6 in Polyethylene Glycol

A novel polyethylene glycol (PEG) gel was fabricated and used as a carrier to immobilize Clostridium butyricum EB6 to improve biohydrogen (bio-H2) production from palm oil mill effluent (POME). POME is used as a substrate that can act as a carbon source. The resulting PEG-immobilized cells were foun...

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Main Authors: Singh, Lakhveer, Zularisam, A. W., Siddique, Md. Nurul Islam, Anwar, Ahmad, Mohd Hasbi, Ab. Rahim, Mimi Sakinah, A. M.
Format: Article
Language:English
Published: Elsevier Ltd 2013
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/6251/1/Biohydrogen%20Production%20from%20Palm%20Oil%20Mill%20Effluent%20Using%20Immobilized%20Clostridium%20Butyricum%20EB6%20in%20Polyethylene%20Glycol.pdf
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author Singh, Lakhveer
Zularisam, A. W.
Siddique, Md. Nurul Islam
Anwar, Ahmad
Mohd Hasbi, Ab. Rahim
Mimi Sakinah, A. M.
author_facet Singh, Lakhveer
Zularisam, A. W.
Siddique, Md. Nurul Islam
Anwar, Ahmad
Mohd Hasbi, Ab. Rahim
Mimi Sakinah, A. M.
author_sort Singh, Lakhveer
collection UMP
description A novel polyethylene glycol (PEG) gel was fabricated and used as a carrier to immobilize Clostridium butyricum EB6 to improve biohydrogen (bio-H2) production from palm oil mill effluent (POME). POME is used as a substrate that can act as a carbon source. The resulting PEG-immobilized cells were found to yield 5.35 LH2/L-POME, and the maximum H2 production rate was 510 mL H2/L-POME h (22.7 mmol/L h). The Monod-type kinetic model was used to describe the effect of substrate (POME) concentration on the H2 production rate. The acclimation of immobilized cells greatly improved H2 production. Batch experiments demonstrated that particle size of PEG-immobilized cells for efficient H2 production 3 mm. It is significant that this is the first report on whole-cell immobilization in PEG for H2 production from POME.
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spelling UMPir62512018-11-22T06:55:50Z http://umpir.ump.edu.my/id/eprint/6251/ Biohydrogen Production from Palm Oil Mill Effluent Using Immobilized Clostridium Butyricum EB6 in Polyethylene Glycol Singh, Lakhveer Zularisam, A. W. Siddique, Md. Nurul Islam Anwar, Ahmad Mohd Hasbi, Ab. Rahim Mimi Sakinah, A. M. Q Science (General) TP Chemical technology TA Engineering (General). Civil engineering (General) A novel polyethylene glycol (PEG) gel was fabricated and used as a carrier to immobilize Clostridium butyricum EB6 to improve biohydrogen (bio-H2) production from palm oil mill effluent (POME). POME is used as a substrate that can act as a carbon source. The resulting PEG-immobilized cells were found to yield 5.35 LH2/L-POME, and the maximum H2 production rate was 510 mL H2/L-POME h (22.7 mmol/L h). The Monod-type kinetic model was used to describe the effect of substrate (POME) concentration on the H2 production rate. The acclimation of immobilized cells greatly improved H2 production. Batch experiments demonstrated that particle size of PEG-immobilized cells for efficient H2 production 3 mm. It is significant that this is the first report on whole-cell immobilization in PEG for H2 production from POME. Elsevier Ltd 2013 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6251/1/Biohydrogen%20Production%20from%20Palm%20Oil%20Mill%20Effluent%20Using%20Immobilized%20Clostridium%20Butyricum%20EB6%20in%20Polyethylene%20Glycol.pdf Singh, Lakhveer and Zularisam, A. W. and Siddique, Md. Nurul Islam and Anwar, Ahmad and Mohd Hasbi, Ab. Rahim and Mimi Sakinah, A. M. (2013) Biohydrogen Production from Palm Oil Mill Effluent Using Immobilized Clostridium Butyricum EB6 in Polyethylene Glycol. Process Biochemistry, 48 (2). pp. 294-298. ISSN 1359-5113. (Published) http://dx.doi.org/10.1016/j.procbio.2012.12.007 DOI: 10.1016/j.procbio.2012.12.007
spellingShingle Q Science (General)
TP Chemical technology
TA Engineering (General). Civil engineering (General)
Singh, Lakhveer
Zularisam, A. W.
Siddique, Md. Nurul Islam
Anwar, Ahmad
Mohd Hasbi, Ab. Rahim
Mimi Sakinah, A. M.
Biohydrogen Production from Palm Oil Mill Effluent Using Immobilized Clostridium Butyricum EB6 in Polyethylene Glycol
title Biohydrogen Production from Palm Oil Mill Effluent Using Immobilized Clostridium Butyricum EB6 in Polyethylene Glycol
title_full Biohydrogen Production from Palm Oil Mill Effluent Using Immobilized Clostridium Butyricum EB6 in Polyethylene Glycol
title_fullStr Biohydrogen Production from Palm Oil Mill Effluent Using Immobilized Clostridium Butyricum EB6 in Polyethylene Glycol
title_full_unstemmed Biohydrogen Production from Palm Oil Mill Effluent Using Immobilized Clostridium Butyricum EB6 in Polyethylene Glycol
title_short Biohydrogen Production from Palm Oil Mill Effluent Using Immobilized Clostridium Butyricum EB6 in Polyethylene Glycol
title_sort biohydrogen production from palm oil mill effluent using immobilized clostridium butyricum eb6 in polyethylene glycol
topic Q Science (General)
TP Chemical technology
TA Engineering (General). Civil engineering (General)
url http://umpir.ump.edu.my/id/eprint/6251/1/Biohydrogen%20Production%20from%20Palm%20Oil%20Mill%20Effluent%20Using%20Immobilized%20Clostridium%20Butyricum%20EB6%20in%20Polyethylene%20Glycol.pdf
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