Purification and Partial Characterization of a Low Molecular Weight L-Asparaginase Produced from Corn Cob Waste
l-asparaginase (E.C.3.5.1.1) is an important enzyme often used to treat acute lymphoblastic leukemia. This paper describes the production, purification and partial characterization of l-asparaginase by Streptobacillus sp. KK2S4. The highest l-asparaginase production was achieved with 0.2% (w/v) of l...
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| Format: | Article |
| Language: | English English |
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Elsevier Ltd
2014
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| Online Access: | http://umpir.ump.edu.my/id/eprint/7034/1/Purification_And_Partial_Characterization_Of_A_Low_Molecular_Weight_L-Asparaginase_Produced_From_Corn_Cob_Waste.pdf http://umpir.ump.edu.my/id/eprint/7034/4/Purification%20and%20Partial%20Characterization%20of%20a%20Low%20Molecular%20Weight%20L-Asparaginase%20Produced%20From%20Corn%20Cob%20Waste.pdf |
| _version_ | 1796990196419395584 |
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| author | Makky, Essam A. Yean , Chun Loh Karim, Md. Rezaul |
| author_facet | Makky, Essam A. Yean , Chun Loh Karim, Md. Rezaul |
| author_sort | Makky, Essam A. |
| collection | UMP |
| description | l-asparaginase (E.C.3.5.1.1) is an important enzyme often used to treat acute lymphoblastic leukemia. This paper describes the production, purification and partial characterization of l-asparaginase by Streptobacillus sp. KK2S4. The highest l-asparaginase production was achieved with 0.2% (w/v) of lactose, 0.1% (w/v) of sodium nitrate, 6% (w/v) of pre-treated corn cob powder and 4% (v/v) of KK2S4 bacterial suspension in 50 ml of MM9 media at pH 5 and 40 °C. The enzyme was purified by ammonium sulfate precipitation followed by DEAE-cellulose and Sephadex G-50 column chromatography. The specific activity of the pure enzyme was recorded to be 21.77 U/mg with 39.58-fold purification and 39% of yield. SDS-PAGE demonstrated a single band with molecular weight of 11.2 kDa. The optimum activity of purified enzyme was recorded at pH 8.5 and 35 °C. This is the first report of a very low molecular weight microbial l-asparaginase produced from corn cob as the main carbon source. |
| first_indexed | 2024-03-06T11:48:03Z |
| format | Article |
| id | UMPir7034 |
| institution | Universiti Malaysia Pahang |
| language | English English |
| last_indexed | 2024-03-06T11:48:03Z |
| publishDate | 2014 |
| publisher | Elsevier Ltd |
| record_format | dspace |
| spelling | UMPir70342018-01-09T07:52:53Z http://umpir.ump.edu.my/id/eprint/7034/ Purification and Partial Characterization of a Low Molecular Weight L-Asparaginase Produced from Corn Cob Waste Makky, Essam A. Yean , Chun Loh Karim, Md. Rezaul Q Science (General) l-asparaginase (E.C.3.5.1.1) is an important enzyme often used to treat acute lymphoblastic leukemia. This paper describes the production, purification and partial characterization of l-asparaginase by Streptobacillus sp. KK2S4. The highest l-asparaginase production was achieved with 0.2% (w/v) of lactose, 0.1% (w/v) of sodium nitrate, 6% (w/v) of pre-treated corn cob powder and 4% (v/v) of KK2S4 bacterial suspension in 50 ml of MM9 media at pH 5 and 40 °C. The enzyme was purified by ammonium sulfate precipitation followed by DEAE-cellulose and Sephadex G-50 column chromatography. The specific activity of the pure enzyme was recorded to be 21.77 U/mg with 39.58-fold purification and 39% of yield. SDS-PAGE demonstrated a single band with molecular weight of 11.2 kDa. The optimum activity of purified enzyme was recorded at pH 8.5 and 35 °C. This is the first report of a very low molecular weight microbial l-asparaginase produced from corn cob as the main carbon source. Elsevier Ltd 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7034/1/Purification_And_Partial_Characterization_Of_A_Low_Molecular_Weight_L-Asparaginase_Produced_From_Corn_Cob_Waste.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/7034/4/Purification%20and%20Partial%20Characterization%20of%20a%20Low%20Molecular%20Weight%20L-Asparaginase%20Produced%20From%20Corn%20Cob%20Waste.pdf Makky, Essam A. and Yean , Chun Loh and Karim, Md. Rezaul (2014) Purification and Partial Characterization of a Low Molecular Weight L-Asparaginase Produced from Corn Cob Waste. Biocatalysis and Agricultural Biotechnology, 3 (4). pp. 265-270. ISSN 1878-8181. (Published) http://dx.doi.org/10.1016/j.bcab.2014.07.004 DOI: 10.1016/j.bcab.2014.07.004 |
| spellingShingle | Q Science (General) Makky, Essam A. Yean , Chun Loh Karim, Md. Rezaul Purification and Partial Characterization of a Low Molecular Weight L-Asparaginase Produced from Corn Cob Waste |
| title | Purification and Partial Characterization of a Low Molecular Weight L-Asparaginase Produced from Corn Cob Waste |
| title_full | Purification and Partial Characterization of a Low Molecular Weight L-Asparaginase Produced from Corn Cob Waste |
| title_fullStr | Purification and Partial Characterization of a Low Molecular Weight L-Asparaginase Produced from Corn Cob Waste |
| title_full_unstemmed | Purification and Partial Characterization of a Low Molecular Weight L-Asparaginase Produced from Corn Cob Waste |
| title_short | Purification and Partial Characterization of a Low Molecular Weight L-Asparaginase Produced from Corn Cob Waste |
| title_sort | purification and partial characterization of a low molecular weight l asparaginase produced from corn cob waste |
| topic | Q Science (General) |
| url | http://umpir.ump.edu.my/id/eprint/7034/1/Purification_And_Partial_Characterization_Of_A_Low_Molecular_Weight_L-Asparaginase_Produced_From_Corn_Cob_Waste.pdf http://umpir.ump.edu.my/id/eprint/7034/4/Purification%20and%20Partial%20Characterization%20of%20a%20Low%20Molecular%20Weight%20L-Asparaginase%20Produced%20From%20Corn%20Cob%20Waste.pdf |
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