Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)

Xylose is found in the embryos of most edible plants. Xylan, the hemicellulose used as raw material in xylose production. Xylanase, the enzyme that is used to hydrolyzed the structure of xylan to produce xylose. This study aims to improve the production of xylose from Palm Oil Empty Fruit Bunch (POE...

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Main Author: Asyuaraa, Sukmarsari
Format: Undergraduates Project Papers
Language:English
Published: 2009
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/775/1/cd4016.pdf
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author Asyuaraa, Sukmarsari
author_facet Asyuaraa, Sukmarsari
author_sort Asyuaraa, Sukmarsari
collection UMP
description Xylose is found in the embryos of most edible plants. Xylan, the hemicellulose used as raw material in xylose production. Xylanase, the enzyme that is used to hydrolyzed the structure of xylan to produce xylose. This study aims to improve the production of xylose from Palm Oil Empty Fruit Bunch (POEFB) using enzymatic hydrolysis process. The effect of cultural conditions such as enzyme concentration and incubation time are used to study the production of xylose. Three different xylanases enzyme are used to produce xylose namely Multifect Xylanase, Multifect CX 12L and Optimase CX 72L. Multifect CX 12L was the best enzyme that yielded the most xylose.The optimization of the production of xylose is carried out by using response surface methodology (RSM) based on the central composite design (CCD). The optimal set cultural conditions for enzyme concentration are 50 U/g and 35.5 hours of incubation. Xylose production of 23.15 mg/ml was achieved by using these optimal conditions.
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spelling UMPir7752015-03-03T07:46:49Z http://umpir.ump.edu.my/id/eprint/775/ Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM) Asyuaraa, Sukmarsari TP Chemical technology Xylose is found in the embryos of most edible plants. Xylan, the hemicellulose used as raw material in xylose production. Xylanase, the enzyme that is used to hydrolyzed the structure of xylan to produce xylose. This study aims to improve the production of xylose from Palm Oil Empty Fruit Bunch (POEFB) using enzymatic hydrolysis process. The effect of cultural conditions such as enzyme concentration and incubation time are used to study the production of xylose. Three different xylanases enzyme are used to produce xylose namely Multifect Xylanase, Multifect CX 12L and Optimase CX 72L. Multifect CX 12L was the best enzyme that yielded the most xylose.The optimization of the production of xylose is carried out by using response surface methodology (RSM) based on the central composite design (CCD). The optimal set cultural conditions for enzyme concentration are 50 U/g and 35.5 hours of incubation. Xylose production of 23.15 mg/ml was achieved by using these optimal conditions. 2009-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/775/1/cd4016.pdf Asyuaraa, Sukmarsari (2009) Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM). Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
spellingShingle TP Chemical technology
Asyuaraa, Sukmarsari
Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)
title Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)
title_full Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)
title_fullStr Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)
title_full_unstemmed Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)
title_short Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)
title_sort effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology rsm
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/775/1/cd4016.pdf
work_keys_str_mv AT asyuaraasukmarsari effectofdifferentenzymesandoptimizationofculturalconditionsonxyloseproductionusingresponsesurfacemethodologyrsm