Enhancement of Hydrogen Production From Palm Oil Mill Effluent Via Cell Immobilisation Technique

Clostridium sp. LS2 was immobilised by entrapment in polyethylene glycol (PEG) gel beads to improve the biohydrogen production rate from palm oil mill effluent (POME). We sought to explore and optimise the hydrogen production capability of the immobilised cells by studying the conditions for cell im...

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Main Authors: Singh, Lakhveer, Zularisam, A. W.
Format: Article
Published: Wiley Online Library 2014
Subjects:
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author Singh, Lakhveer
Zularisam, A. W.
author_facet Singh, Lakhveer
Zularisam, A. W.
author_sort Singh, Lakhveer
collection UMP
description Clostridium sp. LS2 was immobilised by entrapment in polyethylene glycol (PEG) gel beads to improve the biohydrogen production rate from palm oil mill effluent (POME). We sought to explore and optimise the hydrogen production capability of the immobilised cells by studying the conditions for cell immobilisation, including PEG concentration, cell loading and curing times, as well as the effects of temperature and K2HPO4 (500–2000 mg/L), NiCl2 (0.1–5.0 mg/L), FeCl2 (100–400 mg/L) MgSO4 (50–200 mg/L) concentrations on hydrogen production rate. The results showed that by optimising the PEG concentration (10% w/v), initial biomass (2.2 g dry weight), curing time (80 min) and temperature (37 °C), as well as the concentrations of K2HPO4 (2000 mg/L), NiCl2 (1 mg/L), FeCl2 (300 mg/L) and MgSO4 (100 mg/L), a maximum hydrogen production rate of 7.3 L/L-POME/day and a yield of 0.31 L H2/g chemical oxygen demand were obtained during continuous operation. We believe that this process may be potentially expanded for sustained and large-scale hydrogen production. Copyright © 2014 John Wiley & Sons, Ltd.
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spelling UMPir77792018-01-17T02:53:17Z http://umpir.ump.edu.my/id/eprint/7779/ Enhancement of Hydrogen Production From Palm Oil Mill Effluent Via Cell Immobilisation Technique Singh, Lakhveer Zularisam, A. W. T Technology (General) Clostridium sp. LS2 was immobilised by entrapment in polyethylene glycol (PEG) gel beads to improve the biohydrogen production rate from palm oil mill effluent (POME). We sought to explore and optimise the hydrogen production capability of the immobilised cells by studying the conditions for cell immobilisation, including PEG concentration, cell loading and curing times, as well as the effects of temperature and K2HPO4 (500–2000 mg/L), NiCl2 (0.1–5.0 mg/L), FeCl2 (100–400 mg/L) MgSO4 (50–200 mg/L) concentrations on hydrogen production rate. The results showed that by optimising the PEG concentration (10% w/v), initial biomass (2.2 g dry weight), curing time (80 min) and temperature (37 °C), as well as the concentrations of K2HPO4 (2000 mg/L), NiCl2 (1 mg/L), FeCl2 (300 mg/L) and MgSO4 (100 mg/L), a maximum hydrogen production rate of 7.3 L/L-POME/day and a yield of 0.31 L H2/g chemical oxygen demand were obtained during continuous operation. We believe that this process may be potentially expanded for sustained and large-scale hydrogen production. Copyright © 2014 John Wiley & Sons, Ltd. Wiley Online Library 2014 Article PeerReviewed Singh, Lakhveer and Zularisam, A. W. (2014) Enhancement of Hydrogen Production From Palm Oil Mill Effluent Via Cell Immobilisation Technique. International Journal of Energy Research, 2014. pp. 1-8. ISSN 0363-907X (print); 1099-114X (online). (Published) http://dx.doi.org/10.1002/er.3231 DOI: 10.1002/er.3231
spellingShingle T Technology (General)
Singh, Lakhveer
Zularisam, A. W.
Enhancement of Hydrogen Production From Palm Oil Mill Effluent Via Cell Immobilisation Technique
title Enhancement of Hydrogen Production From Palm Oil Mill Effluent Via Cell Immobilisation Technique
title_full Enhancement of Hydrogen Production From Palm Oil Mill Effluent Via Cell Immobilisation Technique
title_fullStr Enhancement of Hydrogen Production From Palm Oil Mill Effluent Via Cell Immobilisation Technique
title_full_unstemmed Enhancement of Hydrogen Production From Palm Oil Mill Effluent Via Cell Immobilisation Technique
title_short Enhancement of Hydrogen Production From Palm Oil Mill Effluent Via Cell Immobilisation Technique
title_sort enhancement of hydrogen production from palm oil mill effluent via cell immobilisation technique
topic T Technology (General)
work_keys_str_mv AT singhlakhveer enhancementofhydrogenproductionfrompalmoilmilleffluentviacellimmobilisationtechnique
AT zularisamaw enhancementofhydrogenproductionfrompalmoilmilleffluentviacellimmobilisationtechnique