Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC)
The purification of papain using a reversed phase expanded bed adsorption chromatography (RP-EBAC)using a FastlineTM 10 EBAC column packed with AmberliteTM XAD7HP has been carried out in this study. An efficient large scale direct recovery of papain was developed and optimized from unclarified papay...
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| Format: | Article |
| Language: | English |
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Elsevier
2014
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| Online Access: | http://umpir.ump.edu.my/id/eprint/8002/1/fkksa-2014-yuhai-%20Purification%20of%20Papain%20from%20Unclarified%20Papaya%20Juice1.pdf |
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| author | Chong, Fui Chin He, Yu Hai Syed Mohd Saufi, Tuan Chik |
| author_facet | Chong, Fui Chin He, Yu Hai Syed Mohd Saufi, Tuan Chik |
| author_sort | Chong, Fui Chin |
| collection | UMP |
| description | The purification of papain using a reversed phase expanded bed adsorption chromatography (RP-EBAC)using a FastlineTM 10 EBAC column packed with AmberliteTM XAD7HP has been carried out in this study. An efficient large scale direct recovery of papain was developed and optimized from unclarified papaya juice feedstock in batch adsorption system. Enhancement of papain purity was further investigated in EBAC by stepwise elution strategy. High papain purity of 74.98% and high purification factor of 7.04 were obtained. This study shows a great potential of using two step elution RP-EBAC system to purify papain from unclarified papaya juice. |
| first_indexed | 2024-03-06T11:50:37Z |
| format | Article |
| id | UMPir8002 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T11:50:37Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | UMPir80022018-04-11T03:37:52Z http://umpir.ump.edu.my/id/eprint/8002/ Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC) Chong, Fui Chin He, Yu Hai Syed Mohd Saufi, Tuan Chik TP Chemical technology The purification of papain using a reversed phase expanded bed adsorption chromatography (RP-EBAC)using a FastlineTM 10 EBAC column packed with AmberliteTM XAD7HP has been carried out in this study. An efficient large scale direct recovery of papain was developed and optimized from unclarified papaya juice feedstock in batch adsorption system. Enhancement of papain purity was further investigated in EBAC by stepwise elution strategy. High papain purity of 74.98% and high purification factor of 7.04 were obtained. This study shows a great potential of using two step elution RP-EBAC system to purify papain from unclarified papaya juice. Elsevier 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/8002/1/fkksa-2014-yuhai-%20Purification%20of%20Papain%20from%20Unclarified%20Papaya%20Juice1.pdf Chong, Fui Chin and He, Yu Hai and Syed Mohd Saufi, Tuan Chik (2014) Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC). Journal of Industrial and Engineering Chemistry, 20 (6). pp. 4293-4297. ISSN 1226-086X. (Published) https://doi.org/10.1016/j.jiec.2014.01.035 doi: 10.1016/j.jiec.2014.01.035 |
| spellingShingle | TP Chemical technology Chong, Fui Chin He, Yu Hai Syed Mohd Saufi, Tuan Chik Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC) |
| title | Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC) |
| title_full | Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC) |
| title_fullStr | Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC) |
| title_full_unstemmed | Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC) |
| title_short | Purification of Papain from Unclarified Papaya Juice using Reversed Phase Expanded Bed Adsorption Chromatography (RP-EBAC) |
| title_sort | purification of papain from unclarified papaya juice using reversed phase expanded bed adsorption chromatography rp ebac |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/8002/1/fkksa-2014-yuhai-%20Purification%20of%20Papain%20from%20Unclarified%20Papaya%20Juice1.pdf |
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