Study on extraction and stability of natural red colorant from hibiscus sabdariffa l.

Hibiscus Sabdariffa L. which is also known as Roselle is a tropical shrub that contains anthocyanins, which is responsible for the red colour appearance of the fruit. The major anthocyanins found in Roselle are delphinidin-3-sambubioside and cyanidin-3-sambubioside, while the minor anthocyanin is cy...

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Main Author: Chan, William
Format: Undergraduates Project Papers
Language:English
Published: 2014
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/9233/1/cd8653.pdf
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author Chan, William
author_facet Chan, William
author_sort Chan, William
collection UMP
description Hibiscus Sabdariffa L. which is also known as Roselle is a tropical shrub that contains anthocyanins, which is responsible for the red colour appearance of the fruit. The major anthocyanins found in Roselle are delphinidin-3-sambubioside and cyanidin-3-sambubioside, while the minor anthocyanin is cyanidin-3-glucoside. Its application in the food industry is wide and includes products such as jam, beverage drink and fruit juice. However, the application of Hibiscus Sabdariffa L. as a source of natural food dye has never been studied. The objective of the study is to use hot water extraction method to extract the anthocyanin from Hibiscus Sabdariffa L. and to study the stability of the extract. Hot water extraction method was used to extract anthocyanin. 4g of dried Roselle calyces and 100mL of distilled water was mixed and the extraction was done using shaking water bath. UV-Vis analysis were done on the samples and results show that the optimal temperature and time found for the extraction process is between the range of 50°C - 60°C and 15 minutes. As for the stability of anthocyanin, the addition of food grade stabilizers such as maltodextrin DE15-20 and trehalose proved that it could retard the degradation of anthocyanin with results showing maltodextrin DE15-20 as the better stabilizer. Lastly, all the samples tested with HPLC showed that cyanidin-3-glucoside were present in all the samples
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spelling UMPir92332021-07-16T00:50:04Z http://umpir.ump.edu.my/id/eprint/9233/ Study on extraction and stability of natural red colorant from hibiscus sabdariffa l. Chan, William TP Chemical technology Hibiscus Sabdariffa L. which is also known as Roselle is a tropical shrub that contains anthocyanins, which is responsible for the red colour appearance of the fruit. The major anthocyanins found in Roselle are delphinidin-3-sambubioside and cyanidin-3-sambubioside, while the minor anthocyanin is cyanidin-3-glucoside. Its application in the food industry is wide and includes products such as jam, beverage drink and fruit juice. However, the application of Hibiscus Sabdariffa L. as a source of natural food dye has never been studied. The objective of the study is to use hot water extraction method to extract the anthocyanin from Hibiscus Sabdariffa L. and to study the stability of the extract. Hot water extraction method was used to extract anthocyanin. 4g of dried Roselle calyces and 100mL of distilled water was mixed and the extraction was done using shaking water bath. UV-Vis analysis were done on the samples and results show that the optimal temperature and time found for the extraction process is between the range of 50°C - 60°C and 15 minutes. As for the stability of anthocyanin, the addition of food grade stabilizers such as maltodextrin DE15-20 and trehalose proved that it could retard the degradation of anthocyanin with results showing maltodextrin DE15-20 as the better stabilizer. Lastly, all the samples tested with HPLC showed that cyanidin-3-glucoside were present in all the samples 2014-07 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9233/1/cd8653.pdf Chan, William (2014) Study on extraction and stability of natural red colorant from hibiscus sabdariffa l. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
spellingShingle TP Chemical technology
Chan, William
Study on extraction and stability of natural red colorant from hibiscus sabdariffa l.
title Study on extraction and stability of natural red colorant from hibiscus sabdariffa l.
title_full Study on extraction and stability of natural red colorant from hibiscus sabdariffa l.
title_fullStr Study on extraction and stability of natural red colorant from hibiscus sabdariffa l.
title_full_unstemmed Study on extraction and stability of natural red colorant from hibiscus sabdariffa l.
title_short Study on extraction and stability of natural red colorant from hibiscus sabdariffa l.
title_sort study on extraction and stability of natural red colorant from hibiscus sabdariffa l
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/9233/1/cd8653.pdf
work_keys_str_mv AT chanwilliam studyonextractionandstabilityofnaturalredcolorantfromhibiscussabdariffal