Characteristics of pulmonary microvascular structure in postnatal yaks
Abstract Yaks are typical plateau-adapted animals, however the microvascular changes and characteristics in their lungs after birth are still unclear. Pulmonary microvasculature characteristics and changes across age groups were analysed using morphological observation and molecular biology detectio...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-97760-z |
| _version_ | 1818344675406249984 |
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| author | Ruidong Wan Ziqi Zhao Min Zhao Ke Hu Jiaxin Zhai Hongxian Yu Qing Wei |
| author_facet | Ruidong Wan Ziqi Zhao Min Zhao Ke Hu Jiaxin Zhai Hongxian Yu Qing Wei |
| author_sort | Ruidong Wan |
| collection | DOAJ |
| description | Abstract Yaks are typical plateau-adapted animals, however the microvascular changes and characteristics in their lungs after birth are still unclear. Pulmonary microvasculature characteristics and changes across age groups were analysed using morphological observation and molecular biology detection in yaks aged 1, 30 and 180 days old in addition to adults. Results: Our experiments demonstrated that yaks have fully developed pulmonary alveolar at birth but that interalveolar thickness increased with age. Immunofluorescence observations showed that microvessel density within the interalveolar septum in the yak gradually increased with age. In addition, transmission electron microscopy (TEM) results showed that the blood–air barrier of 1-day old and 30-days old yaks was significantly thicker than that observed at 180-days old and in adults (P < 0.05), which was caused by the thinning of the membrane of alveolar epithelial cells. Furthermore, Vegfa and Epas1 expression levels in 30-day old yaks were the highest in comparison to the other age groups (P < 0.05), whilst levels in adult yaks were the lowest (P < 0.05). The gradual increase in lung microvessel density can effectively satisfy the oxygen requirements of ageing yaks. In addition, these results suggest that the key period of yak lung development is from 30 to 180 days. |
| first_indexed | 2024-12-13T16:50:15Z |
| format | Article |
| id | doaj.art-00019abe98ca428daf529e50a4930a17 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T16:50:15Z |
| publishDate | 2021-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-00019abe98ca428daf529e50a4930a172022-12-21T23:38:03ZengNature PortfolioScientific Reports2045-23222021-09-011111710.1038/s41598-021-97760-zCharacteristics of pulmonary microvascular structure in postnatal yaksRuidong Wan0Ziqi Zhao1Min Zhao2Ke Hu3Jiaxin Zhai4Hongxian Yu5Qing Wei6Department of Veterinary Medicine, College of Agriculture and Animal Husbandry, Qinghai UniversityCollege of Eco-Environmental Engineering, Qinghai UniversityCollege of Eco-Environmental Engineering, Qinghai UniversityCollege of Eco-Environmental Engineering, Qinghai UniversityCollege of Eco-Environmental Engineering, Qinghai UniversityDepartment of Veterinary Medicine, College of Agriculture and Animal Husbandry, Qinghai UniversityCollege of Eco-Environmental Engineering, Qinghai UniversityAbstract Yaks are typical plateau-adapted animals, however the microvascular changes and characteristics in their lungs after birth are still unclear. Pulmonary microvasculature characteristics and changes across age groups were analysed using morphological observation and molecular biology detection in yaks aged 1, 30 and 180 days old in addition to adults. Results: Our experiments demonstrated that yaks have fully developed pulmonary alveolar at birth but that interalveolar thickness increased with age. Immunofluorescence observations showed that microvessel density within the interalveolar septum in the yak gradually increased with age. In addition, transmission electron microscopy (TEM) results showed that the blood–air barrier of 1-day old and 30-days old yaks was significantly thicker than that observed at 180-days old and in adults (P < 0.05), which was caused by the thinning of the membrane of alveolar epithelial cells. Furthermore, Vegfa and Epas1 expression levels in 30-day old yaks were the highest in comparison to the other age groups (P < 0.05), whilst levels in adult yaks were the lowest (P < 0.05). The gradual increase in lung microvessel density can effectively satisfy the oxygen requirements of ageing yaks. In addition, these results suggest that the key period of yak lung development is from 30 to 180 days.https://doi.org/10.1038/s41598-021-97760-z |
| spellingShingle | Ruidong Wan Ziqi Zhao Min Zhao Ke Hu Jiaxin Zhai Hongxian Yu Qing Wei Characteristics of pulmonary microvascular structure in postnatal yaks Scientific Reports |
| title | Characteristics of pulmonary microvascular structure in postnatal yaks |
| title_full | Characteristics of pulmonary microvascular structure in postnatal yaks |
| title_fullStr | Characteristics of pulmonary microvascular structure in postnatal yaks |
| title_full_unstemmed | Characteristics of pulmonary microvascular structure in postnatal yaks |
| title_short | Characteristics of pulmonary microvascular structure in postnatal yaks |
| title_sort | characteristics of pulmonary microvascular structure in postnatal yaks |
| url | https://doi.org/10.1038/s41598-021-97760-z |
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