Encapsulation of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor in liposomes prepared by thin film hydration and their transfer to mesenchymal stem cells and cord blood hematopoietic stem cells
Introduction Cytokines are important immune modulator factors controlling homeostasis of the body and are involved in tissue regeneration after wound healing. The encapsulation of cytokines in liposomes has many advantages potentially useful for their transfer to the cells. Liposomes protect cytokin...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2020-04-01
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| Series: | Archives of Medical Science |
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| Online Access: | https://www.archivesofmedicalscience.com/Encapsulation-of-granulocyte-colony-stimulating-factor-and-granulocyte-macrophage,110381,0,2.html |
| _version_ | 1797435290540834816 |
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| author | Sławomir Lewicki Monika Leśniak Agnieszka Sobolewska-Ruta Aneta Lewicka Marta Grodzik Eugeniusz K. Machaj Marek Saracyn Jacek Z. Kubiak Zygmunt Pojda |
| author_facet | Sławomir Lewicki Monika Leśniak Agnieszka Sobolewska-Ruta Aneta Lewicka Marta Grodzik Eugeniusz K. Machaj Marek Saracyn Jacek Z. Kubiak Zygmunt Pojda |
| author_sort | Sławomir Lewicki |
| collection | DOAJ |
| description | Introduction
Cytokines are important immune modulator factors controlling homeostasis of the body and are involved in tissue regeneration after wound healing. The encapsulation of cytokines in liposomes has many advantages potentially useful for their transfer to the cells. Liposomes protect cytokines from neutralization, improving their pharmacokinetics or biologic activity in vivo. They are targeted to specific cell types and may delay the release of cytokines, allowing their sustained paracrine delivery. Their physicochemical characteristics such as size, shape, charge, and stability are important parameters improving bio-distribution and prolonged pharmacokinetics of encapsulated cytokines.
Material and methods
We developed an efficient protocol for the encapsulation of two types of cytokines, granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF), in liposomes that can be stored long term in the active state.
Results
This method allows for the encapsulation of 12–13% of the total amount of cytokines and 50% of encapsulated cytokines are entrapped in liposomes of more than ≤ 600 nm in diameter. We show that in the studied cell lines the liposome-encapsulated cytokines do not affect cell morphology, proliferation or mortality.
Conclusions
The G-CSF or GM-CSF can be delivered to the cells in working concentrations through the encapsulation in the liposomes. Before the clinical application, the efficiency of these liposomes should be confirmed by an in vivo study. |
| first_indexed | 2024-03-09T10:46:02Z |
| format | Article |
| id | doaj.art-00028bf0fddf4b5385598ac8f16d8d40 |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-03-09T10:46:02Z |
| publishDate | 2020-04-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-00028bf0fddf4b5385598ac8f16d8d402023-12-01T10:02:33ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512020-04-011841051106110.5114/aoms.2020.94527110381Encapsulation of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor in liposomes prepared by thin film hydration and their transfer to mesenchymal stem cells and cord blood hematopoietic stem cellsSławomir Lewicki0Monika Leśniak1Agnieszka Sobolewska-Ruta2Aneta Lewicka3Marta Grodzik4Eugeniusz K. Machaj5Marek Saracyn6Jacek Z. Kubiak7Zygmunt Pojda8Department of Regenerative Medicine and Cell Biology, Military Institute of Hygiene and Epidemiology, Warsaw, PolandDepartment of Regenerative Medicine and Cell Biology, Military Institute of Hygiene and Epidemiology, Warsaw, PolandBioengineering Department, The Institute of Biotechnology and Antibiotics, Warsaw, PolandLaboratory of Food and Nutrition Hygiene, Military Institute of Hygiene and Epidemiology, Warsaw, PolandDivision of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, Warsaw, PolandPolish Stem Cell Bank, Warsaw, PolandDepartment of Endocrinology and Isotope Therapy, Military Institute of Medicine, Warsaw, PolandDepartment of Regenerative Medicine and Cell Biology, Military Institute of Hygiene and Epidemiology, Warsaw, PolandDepartment of Regenerative Medicine, Maria Skłodowska-Curie Institute-Oncology Center, Warsaw, PolandIntroduction Cytokines are important immune modulator factors controlling homeostasis of the body and are involved in tissue regeneration after wound healing. The encapsulation of cytokines in liposomes has many advantages potentially useful for their transfer to the cells. Liposomes protect cytokines from neutralization, improving their pharmacokinetics or biologic activity in vivo. They are targeted to specific cell types and may delay the release of cytokines, allowing their sustained paracrine delivery. Their physicochemical characteristics such as size, shape, charge, and stability are important parameters improving bio-distribution and prolonged pharmacokinetics of encapsulated cytokines. Material and methods We developed an efficient protocol for the encapsulation of two types of cytokines, granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF), in liposomes that can be stored long term in the active state. Results This method allows for the encapsulation of 12–13% of the total amount of cytokines and 50% of encapsulated cytokines are entrapped in liposomes of more than ≤ 600 nm in diameter. We show that in the studied cell lines the liposome-encapsulated cytokines do not affect cell morphology, proliferation or mortality. Conclusions The G-CSF or GM-CSF can be delivered to the cells in working concentrations through the encapsulation in the liposomes. Before the clinical application, the efficiency of these liposomes should be confirmed by an in vivo study.https://www.archivesofmedicalscience.com/Encapsulation-of-granulocyte-colony-stimulating-factor-and-granulocyte-macrophage,110381,0,2.htmlliposomesgranulocyte colony-stimulating factorgranulocyte- macrophage colony-stimulating factordelivery to stem cellsprotein transfer to cells in vitro |
| spellingShingle | Sławomir Lewicki Monika Leśniak Agnieszka Sobolewska-Ruta Aneta Lewicka Marta Grodzik Eugeniusz K. Machaj Marek Saracyn Jacek Z. Kubiak Zygmunt Pojda Encapsulation of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor in liposomes prepared by thin film hydration and their transfer to mesenchymal stem cells and cord blood hematopoietic stem cells Archives of Medical Science liposomes granulocyte colony-stimulating factor granulocyte- macrophage colony-stimulating factor delivery to stem cells protein transfer to cells in vitro |
| title | Encapsulation of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor in liposomes prepared by thin film hydration and their transfer to mesenchymal stem cells and cord blood hematopoietic stem cells |
| title_full | Encapsulation of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor in liposomes prepared by thin film hydration and their transfer to mesenchymal stem cells and cord blood hematopoietic stem cells |
| title_fullStr | Encapsulation of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor in liposomes prepared by thin film hydration and their transfer to mesenchymal stem cells and cord blood hematopoietic stem cells |
| title_full_unstemmed | Encapsulation of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor in liposomes prepared by thin film hydration and their transfer to mesenchymal stem cells and cord blood hematopoietic stem cells |
| title_short | Encapsulation of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor in liposomes prepared by thin film hydration and their transfer to mesenchymal stem cells and cord blood hematopoietic stem cells |
| title_sort | encapsulation of granulocyte colony stimulating factor and granulocyte macrophage colony stimulating factor in liposomes prepared by thin film hydration and their transfer to mesenchymal stem cells and cord blood hematopoietic stem cells |
| topic | liposomes granulocyte colony-stimulating factor granulocyte- macrophage colony-stimulating factor delivery to stem cells protein transfer to cells in vitro |
| url | https://www.archivesofmedicalscience.com/Encapsulation-of-granulocyte-colony-stimulating-factor-and-granulocyte-macrophage,110381,0,2.html |
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