Draft genome sequences of two hypermucoviscous carbapenem-resistant ST25 Klebsiella pneumoniae strains causing respiratory and systemic infections

ABSTRACT: Objectives: The emergence and spread of hypermucoviscous KPC-2-producing Klebsiella pneumoniae strains belonging to the sequence type 25 (ST25) clone was reported recently in Northwest Argentina as a leading cause of nosocomial infections. The aim of this work was to perform whole-genome s...

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Main Authors: María Angela Jure, Leonardo Albarracin, Juan Martin Vargas, Stefania Dentice Maidana, Juan Cortez Zamar, Haruki Kitazawa, Julio Villena
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:Journal of Global Antimicrobial Resistance
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2213716521001491
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author María Angela Jure
Leonardo Albarracin
Juan Martin Vargas
Stefania Dentice Maidana
Juan Cortez Zamar
Haruki Kitazawa
Julio Villena
author_facet María Angela Jure
Leonardo Albarracin
Juan Martin Vargas
Stefania Dentice Maidana
Juan Cortez Zamar
Haruki Kitazawa
Julio Villena
author_sort María Angela Jure
collection DOAJ
description ABSTRACT: Objectives: The emergence and spread of hypermucoviscous KPC-2-producing Klebsiella pneumoniae strains belonging to the sequence type 25 (ST25) clone was reported recently in Northwest Argentina as a leading cause of nosocomial infections. The aim of this work was to perform whole-genome sequencing (WGS) to analyse antimicrobial resistance genes (ARGs), virulence factors and colonisation-associated genes in two carbapenem-resistant KPC-2-producing ST25 K. pneumoniae strains isolated from hospitalised patients.Methods: Classical microbiological methods were applied to recover K. pneumoniae LABACER 01 from a bone sample and LABACER 27 from the respiratory tract of two hospitalised patients. Bacteria were identified by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF). WGS was performed using an Illumina MiSeq platform. Genome annotation and analysis were performed with available databases and bioinformatic tools.Results: Genomic analysis revealed a genome of 5 598 020 bp with 19 further characterised ARGs in strain LABACER 01, and a genome of 5 622 382 bp with 20 ARGs in strain LABACER 27. Bioinformatics analysis also predicted genomic regions associated with virulence factors and mucosal tissue colonisation.Conclusion: This study reports the genomic analysis of K. pneumoniae LABACER 01 and LABACER 27, two hypermucoviscous carbapenem-resistant ST25 strains, which expands our knowledge on the antibiotic resistance, pathogenic mechanisms and biology of ST25 clones recently emerging in Argentina.
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spelling doaj.art-0004d8ffaadf419cb425fa0d9cc3b1402022-12-21T18:51:21ZengElsevierJournal of Global Antimicrobial Resistance2213-71652021-09-0126174176Draft genome sequences of two hypermucoviscous carbapenem-resistant ST25 Klebsiella pneumoniae strains causing respiratory and systemic infectionsMaría Angela Jure0Leonardo Albarracin1Juan Martin Vargas2Stefania Dentice Maidana3Juan Cortez Zamar4Haruki Kitazawa5Julio Villena6Laboratory of Antimicrobials, Institute of Microbiology ‘Luis C. Verna’, Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucumán, Tucumán, ArgentinaLaboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucumán, Argentina; Scientific Computing Laboratory, Computer Science Department, Faculty of Exact Science and Technology, National University of Tucumán, Tucumán, Argentina; Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, JapanLaboratory of Antimicrobials, Institute of Microbiology ‘Luis C. Verna’, Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucumán, Tucumán, ArgentinaLaboratory of Antimicrobials, Institute of Microbiology ‘Luis C. Verna’, Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucumán, Tucumán, Argentina; Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucumán, Argentina; Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, JapanLaboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucumán, ArgentinaFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan; Livestock Immunology Unit, International Education and Research Centre for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan; Corresponding authors.Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucumán, Argentina; Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan; Corresponding authors.ABSTRACT: Objectives: The emergence and spread of hypermucoviscous KPC-2-producing Klebsiella pneumoniae strains belonging to the sequence type 25 (ST25) clone was reported recently in Northwest Argentina as a leading cause of nosocomial infections. The aim of this work was to perform whole-genome sequencing (WGS) to analyse antimicrobial resistance genes (ARGs), virulence factors and colonisation-associated genes in two carbapenem-resistant KPC-2-producing ST25 K. pneumoniae strains isolated from hospitalised patients.Methods: Classical microbiological methods were applied to recover K. pneumoniae LABACER 01 from a bone sample and LABACER 27 from the respiratory tract of two hospitalised patients. Bacteria were identified by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF). WGS was performed using an Illumina MiSeq platform. Genome annotation and analysis were performed with available databases and bioinformatic tools.Results: Genomic analysis revealed a genome of 5 598 020 bp with 19 further characterised ARGs in strain LABACER 01, and a genome of 5 622 382 bp with 20 ARGs in strain LABACER 27. Bioinformatics analysis also predicted genomic regions associated with virulence factors and mucosal tissue colonisation.Conclusion: This study reports the genomic analysis of K. pneumoniae LABACER 01 and LABACER 27, two hypermucoviscous carbapenem-resistant ST25 strains, which expands our knowledge on the antibiotic resistance, pathogenic mechanisms and biology of ST25 clones recently emerging in Argentina.http://www.sciencedirect.com/science/article/pii/S2213716521001491HypermucoviscousCarbapenem-resistant Klebsiella pneumoniaeGenomeAntimicrobial resistanceST25
spellingShingle María Angela Jure
Leonardo Albarracin
Juan Martin Vargas
Stefania Dentice Maidana
Juan Cortez Zamar
Haruki Kitazawa
Julio Villena
Draft genome sequences of two hypermucoviscous carbapenem-resistant ST25 Klebsiella pneumoniae strains causing respiratory and systemic infections
Journal of Global Antimicrobial Resistance
Hypermucoviscous
Carbapenem-resistant Klebsiella pneumoniae
Genome
Antimicrobial resistance
ST25
title Draft genome sequences of two hypermucoviscous carbapenem-resistant ST25 Klebsiella pneumoniae strains causing respiratory and systemic infections
title_full Draft genome sequences of two hypermucoviscous carbapenem-resistant ST25 Klebsiella pneumoniae strains causing respiratory and systemic infections
title_fullStr Draft genome sequences of two hypermucoviscous carbapenem-resistant ST25 Klebsiella pneumoniae strains causing respiratory and systemic infections
title_full_unstemmed Draft genome sequences of two hypermucoviscous carbapenem-resistant ST25 Klebsiella pneumoniae strains causing respiratory and systemic infections
title_short Draft genome sequences of two hypermucoviscous carbapenem-resistant ST25 Klebsiella pneumoniae strains causing respiratory and systemic infections
title_sort draft genome sequences of two hypermucoviscous carbapenem resistant st25 klebsiella pneumoniae strains causing respiratory and systemic infections
topic Hypermucoviscous
Carbapenem-resistant Klebsiella pneumoniae
Genome
Antimicrobial resistance
ST25
url http://www.sciencedirect.com/science/article/pii/S2213716521001491
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