A Fully Adapted Headstage With Custom Electrode Arrays Designed for Electrophysiological Experiments
Electrophysiological recordings lead amongst the techniques that aim to investigate the dynamics of neural activity sampled from large neural ensembles. However, the financial costs associated with the state-of-the-art technology used to manufacture probes and multi-channel recording systems make th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2021.691788/full |
| _version_ | 1818488945794613248 |
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| author | Flávio Afonso Gonçalves Mourão Leonardo de Oliveira Guarnieri Leonardo de Oliveira Guarnieri Paulo Aparecido Amaral Júnior Paulo Aparecido Amaral Júnior Vinícius Rezende Carvalho Vinícius Rezende Carvalho Eduardo Mazoni Andrade Marçal Mendes Eduardo Mazoni Andrade Marçal Mendes Márcio Flávio Dutra Moraes Márcio Flávio Dutra Moraes |
| author_facet | Flávio Afonso Gonçalves Mourão Leonardo de Oliveira Guarnieri Leonardo de Oliveira Guarnieri Paulo Aparecido Amaral Júnior Paulo Aparecido Amaral Júnior Vinícius Rezende Carvalho Vinícius Rezende Carvalho Eduardo Mazoni Andrade Marçal Mendes Eduardo Mazoni Andrade Marçal Mendes Márcio Flávio Dutra Moraes Márcio Flávio Dutra Moraes |
| author_sort | Flávio Afonso Gonçalves Mourão |
| collection | DOAJ |
| description | Electrophysiological recordings lead amongst the techniques that aim to investigate the dynamics of neural activity sampled from large neural ensembles. However, the financial costs associated with the state-of-the-art technology used to manufacture probes and multi-channel recording systems make these experiments virtually inaccessible to small laboratories, especially if located in developing countries. Here, we describe a new method for implanting several tungsten electrode arrays, widely distributed over the brain. Moreover, we designed a headstage system, using the Intan® RHD2000 chipset, associated with a connector (replacing the expensive commercial Omnetics connector), that allows the usage of disposable and inexpensive cranial implants. Our results showed high-quality multichannel recording in freely moving animals (detecting local field, evoked responses and unit activities) and robust mechanical connections ensuring long-term continuous recordings. Our project represents an open source and inexpensive alternative to develop customized extracellular records from multiple brain regions. |
| first_indexed | 2024-12-10T16:57:34Z |
| format | Article |
| id | doaj.art-000804b4b44d4ba9ad2215d9798a22ec |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-10T16:57:34Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-000804b4b44d4ba9ad2215d9798a22ec2022-12-22T01:40:40ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-03-011510.3389/fnins.2021.691788691788A Fully Adapted Headstage With Custom Electrode Arrays Designed for Electrophysiological ExperimentsFlávio Afonso Gonçalves Mourão0Leonardo de Oliveira Guarnieri1Leonardo de Oliveira Guarnieri2Paulo Aparecido Amaral Júnior3Paulo Aparecido Amaral Júnior4Vinícius Rezende Carvalho5Vinícius Rezende Carvalho6Eduardo Mazoni Andrade Marçal Mendes7Eduardo Mazoni Andrade Marçal Mendes8Márcio Flávio Dutra Moraes9Márcio Flávio Dutra Moraes10Programa de Pós Graduação em Neurociências, Núcleo de Neurociências, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, BrazilPrograma de Pós Graduação em Neurociências, Núcleo de Neurociências, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, BrazilPrograma de Pós-Graduação em Engenharia Elétrica, Departamento de Engenharia Eletrônica (DELT), Escola de Engenharia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, BrazilPrograma de Pós Graduação em Neurociências, Núcleo de Neurociências, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, BrazilPrograma de Pós-Graduação em Engenharia Elétrica, Departamento de Engenharia Eletrônica (DELT), Escola de Engenharia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, BrazilPrograma de Pós Graduação em Neurociências, Núcleo de Neurociências, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, BrazilPrograma de Pós-Graduação em Engenharia Elétrica, Departamento de Engenharia Eletrônica (DELT), Escola de Engenharia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, BrazilPrograma de Pós Graduação em Neurociências, Núcleo de Neurociências, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, BrazilPrograma de Pós-Graduação em Engenharia Elétrica, Departamento de Engenharia Eletrônica (DELT), Escola de Engenharia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, BrazilPrograma de Pós Graduação em Neurociências, Núcleo de Neurociências, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, BrazilPrograma de Pós-Graduação em Engenharia Elétrica, Departamento de Engenharia Eletrônica (DELT), Escola de Engenharia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, BrazilElectrophysiological recordings lead amongst the techniques that aim to investigate the dynamics of neural activity sampled from large neural ensembles. However, the financial costs associated with the state-of-the-art technology used to manufacture probes and multi-channel recording systems make these experiments virtually inaccessible to small laboratories, especially if located in developing countries. Here, we describe a new method for implanting several tungsten electrode arrays, widely distributed over the brain. Moreover, we designed a headstage system, using the Intan® RHD2000 chipset, associated with a connector (replacing the expensive commercial Omnetics connector), that allows the usage of disposable and inexpensive cranial implants. Our results showed high-quality multichannel recording in freely moving animals (detecting local field, evoked responses and unit activities) and robust mechanical connections ensuring long-term continuous recordings. Our project represents an open source and inexpensive alternative to develop customized extracellular records from multiple brain regions.https://www.frontiersin.org/articles/10.3389/fnins.2021.691788/fullextracellular electrophysiology recordinglaboratory equipmentIntan Technologieselectrode arraystungsten wires |
| spellingShingle | Flávio Afonso Gonçalves Mourão Leonardo de Oliveira Guarnieri Leonardo de Oliveira Guarnieri Paulo Aparecido Amaral Júnior Paulo Aparecido Amaral Júnior Vinícius Rezende Carvalho Vinícius Rezende Carvalho Eduardo Mazoni Andrade Marçal Mendes Eduardo Mazoni Andrade Marçal Mendes Márcio Flávio Dutra Moraes Márcio Flávio Dutra Moraes A Fully Adapted Headstage With Custom Electrode Arrays Designed for Electrophysiological Experiments Frontiers in Neuroscience extracellular electrophysiology recording laboratory equipment Intan Technologies electrode arrays tungsten wires |
| title | A Fully Adapted Headstage With Custom Electrode Arrays Designed for Electrophysiological Experiments |
| title_full | A Fully Adapted Headstage With Custom Electrode Arrays Designed for Electrophysiological Experiments |
| title_fullStr | A Fully Adapted Headstage With Custom Electrode Arrays Designed for Electrophysiological Experiments |
| title_full_unstemmed | A Fully Adapted Headstage With Custom Electrode Arrays Designed for Electrophysiological Experiments |
| title_short | A Fully Adapted Headstage With Custom Electrode Arrays Designed for Electrophysiological Experiments |
| title_sort | fully adapted headstage with custom electrode arrays designed for electrophysiological experiments |
| topic | extracellular electrophysiology recording laboratory equipment Intan Technologies electrode arrays tungsten wires |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2021.691788/full |
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