A cross-sectional cohort study of the activity and turnover of neutrophil granulocytes in juvenile idiopathic arthritis
Abstract Background The inflammatory process in juvenile idiopathic arthritis (JIA) involves both the innate and the adaptive immune system. The turnover and activity of neutrophil granulocytes may be reflected by proteins secreted from primary or secondary granules and from the cytoplasm of sequest...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-06-01
|
| Series: | Pediatric Rheumatology Online Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12969-021-00600-7 |
| _version_ | 1818907575105617920 |
|---|---|
| author | Malin Backlund Per Venge Lillemor Berntson |
| author_facet | Malin Backlund Per Venge Lillemor Berntson |
| author_sort | Malin Backlund |
| collection | DOAJ |
| description | Abstract Background The inflammatory process in juvenile idiopathic arthritis (JIA) involves both the innate and the adaptive immune system. The turnover and activity of neutrophil granulocytes may be reflected by proteins secreted from primary or secondary granules and from the cytoplasm of sequestered cells. Our primary aim was to compare the levels of the secondary neutrophil granule protein human neutrophil lipocalin (HNL), in JIA patients and controls, and to explore a possible priming of neutrophils through parallel analyses in plasma and serum. A secondary aim was to relate the levels of HNL to two other well-studied leukocyte proteins, S100A8/A9 and myeloperoxidase (MPO), as well as to clinical aspects of JIA. Methods The concentrations of the three biomarkers in serum, two of them also in plasma, were measured using enzyme-linked immunosorbent assay in 37 children with JIA without medical treatment, in high disease activity based on juvenile arthritis disease activity score 27 (JADAS27), 32 children on medical treatment, mainly in lower disease activity, and 16 healthy children. We assessed for differences between two groups using the Mann-Whitney U test, and used the Kruskal-Wallis test for multiple group comparisons. Spearman rank correlation, linear and multiple regression analyses were used for evaluation of associations between biomarker concentrations and clinical scores. Results The concentrations of HNL and MPO in serum were significantly increased in children with JIA (p < 0.001, p = 0.002) compared with healthy children, but we found no difference in the plasma levels of HNL and MPO between children with JIA and controls. The serum concentrations of MPO and HNL were unaffected by medical treatment, but S100A8/A9 was reduced by medical treatment and correlated with JADAS27 in both univariate (r = 0.58, p < 0.001) and multivariate (r = 0.59, p < 0.001) analyses. Conclusions Neutrophil granulocytes in children with JIA are primed to release primary and secondary granule proteins, without relation to medical treatment, whereas signs of increased turnover and sequestration of neutrophil granulocytes are reduced by treatment. Levels of neutrophil-originating proteins in serum most likely reflect underlying disease activities of JIA. |
| first_indexed | 2024-12-19T21:57:18Z |
| format | Article |
| id | doaj.art-00090c2179fd4562bfec0bcc4b691e12 |
| institution | Directory Open Access Journal |
| issn | 1546-0096 |
| language | English |
| last_indexed | 2024-12-19T21:57:18Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Pediatric Rheumatology Online Journal |
| spelling | doaj.art-00090c2179fd4562bfec0bcc4b691e122022-12-21T20:04:15ZengBMCPediatric Rheumatology Online Journal1546-00962021-06-011911810.1186/s12969-021-00600-7A cross-sectional cohort study of the activity and turnover of neutrophil granulocytes in juvenile idiopathic arthritisMalin Backlund0Per Venge1Lillemor Berntson2Department of Women’s and Children’s Health, Uppsala UniversityDepartment of Medical Sciences, Uppsala UniversityDepartment of Women’s and Children’s Health, Uppsala UniversityAbstract Background The inflammatory process in juvenile idiopathic arthritis (JIA) involves both the innate and the adaptive immune system. The turnover and activity of neutrophil granulocytes may be reflected by proteins secreted from primary or secondary granules and from the cytoplasm of sequestered cells. Our primary aim was to compare the levels of the secondary neutrophil granule protein human neutrophil lipocalin (HNL), in JIA patients and controls, and to explore a possible priming of neutrophils through parallel analyses in plasma and serum. A secondary aim was to relate the levels of HNL to two other well-studied leukocyte proteins, S100A8/A9 and myeloperoxidase (MPO), as well as to clinical aspects of JIA. Methods The concentrations of the three biomarkers in serum, two of them also in plasma, were measured using enzyme-linked immunosorbent assay in 37 children with JIA without medical treatment, in high disease activity based on juvenile arthritis disease activity score 27 (JADAS27), 32 children on medical treatment, mainly in lower disease activity, and 16 healthy children. We assessed for differences between two groups using the Mann-Whitney U test, and used the Kruskal-Wallis test for multiple group comparisons. Spearman rank correlation, linear and multiple regression analyses were used for evaluation of associations between biomarker concentrations and clinical scores. Results The concentrations of HNL and MPO in serum were significantly increased in children with JIA (p < 0.001, p = 0.002) compared with healthy children, but we found no difference in the plasma levels of HNL and MPO between children with JIA and controls. The serum concentrations of MPO and HNL were unaffected by medical treatment, but S100A8/A9 was reduced by medical treatment and correlated with JADAS27 in both univariate (r = 0.58, p < 0.001) and multivariate (r = 0.59, p < 0.001) analyses. Conclusions Neutrophil granulocytes in children with JIA are primed to release primary and secondary granule proteins, without relation to medical treatment, whereas signs of increased turnover and sequestration of neutrophil granulocytes are reduced by treatment. Levels of neutrophil-originating proteins in serum most likely reflect underlying disease activities of JIA.https://doi.org/10.1186/s12969-021-00600-7Neutrophil granulocytesPrimingGranule proteinsJuvenile idiopathic arthritis |
| spellingShingle | Malin Backlund Per Venge Lillemor Berntson A cross-sectional cohort study of the activity and turnover of neutrophil granulocytes in juvenile idiopathic arthritis Pediatric Rheumatology Online Journal Neutrophil granulocytes Priming Granule proteins Juvenile idiopathic arthritis |
| title | A cross-sectional cohort study of the activity and turnover of neutrophil granulocytes in juvenile idiopathic arthritis |
| title_full | A cross-sectional cohort study of the activity and turnover of neutrophil granulocytes in juvenile idiopathic arthritis |
| title_fullStr | A cross-sectional cohort study of the activity and turnover of neutrophil granulocytes in juvenile idiopathic arthritis |
| title_full_unstemmed | A cross-sectional cohort study of the activity and turnover of neutrophil granulocytes in juvenile idiopathic arthritis |
| title_short | A cross-sectional cohort study of the activity and turnover of neutrophil granulocytes in juvenile idiopathic arthritis |
| title_sort | cross sectional cohort study of the activity and turnover of neutrophil granulocytes in juvenile idiopathic arthritis |
| topic | Neutrophil granulocytes Priming Granule proteins Juvenile idiopathic arthritis |
| url | https://doi.org/10.1186/s12969-021-00600-7 |
| work_keys_str_mv | AT malinbacklund acrosssectionalcohortstudyoftheactivityandturnoverofneutrophilgranulocytesinjuvenileidiopathicarthritis AT pervenge acrosssectionalcohortstudyoftheactivityandturnoverofneutrophilgranulocytesinjuvenileidiopathicarthritis AT lillemorberntson acrosssectionalcohortstudyoftheactivityandturnoverofneutrophilgranulocytesinjuvenileidiopathicarthritis AT malinbacklund crosssectionalcohortstudyoftheactivityandturnoverofneutrophilgranulocytesinjuvenileidiopathicarthritis AT pervenge crosssectionalcohortstudyoftheactivityandturnoverofneutrophilgranulocytesinjuvenileidiopathicarthritis AT lillemorberntson crosssectionalcohortstudyoftheactivityandturnoverofneutrophilgranulocytesinjuvenileidiopathicarthritis |