The Proteome of Circulating Large Extracellular Vesicles in Diabetes and Hypertension
Hypertension and diabetes induce vascular injury through processes that are not fully understood. Changes in extracellular vesicle (EV) composition could provide novel insights. Here, we examined the protein composition of circulating EVs from hypertensive, diabetic and healthy mice. EVs were isolat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/5/4930 |
| _version_ | 1797615062702096384 |
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| author | Akram Abolbaghaei Maddison Turner Jean-François Thibodeau Chet E. Holterman Christopher R. J. Kennedy Dylan Burger |
| author_facet | Akram Abolbaghaei Maddison Turner Jean-François Thibodeau Chet E. Holterman Christopher R. J. Kennedy Dylan Burger |
| author_sort | Akram Abolbaghaei |
| collection | DOAJ |
| description | Hypertension and diabetes induce vascular injury through processes that are not fully understood. Changes in extracellular vesicle (EV) composition could provide novel insights. Here, we examined the protein composition of circulating EVs from hypertensive, diabetic and healthy mice. EVs were isolated from transgenic mice overexpressing human renin in the liver (TtRhRen, hypertensive), OVE26 type 1 diabetic mice and wild-type (WT) mice. Protein content was analyzed using liquid chromatography–mass spectrometry. We identified 544 independent proteins, of which 408 were found in all groups, 34 were exclusive to WT, 16 were exclusive to OVE26 and 5 were exclusive to TTRhRen mice. Amongst the differentially expressed proteins, haptoglobin (HPT) was upregulated and ankyrin-1 (ANK1) was downregulated in OVE26 and TtRhRen mice compared with WT controls. Conversely, TSP4 and Co3A1 were upregulated and SAA4 was downregulated exclusively in diabetic mice; and PPN was upregulated and SPTB1 and SPTA1 were downregulated in hypertensive mice, compared to WT mice. Ingenuity pathway analysis identified enrichment in proteins associated with SNARE signaling, the complement system and NAD homeostasis in EVs from diabetic mice. Conversely, in EVs from hypertensive mice, there was enrichment in semaphroin and Rho signaling. Further analysis of these changes may improve understanding of vascular injury in hypertension and diabetes. |
| first_indexed | 2024-03-11T07:21:10Z |
| format | Article |
| id | doaj.art-000df9d8ad184fd6a8b3e5ad876b13c2 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T07:21:10Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-000df9d8ad184fd6a8b3e5ad876b13c22023-11-17T07:55:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01245493010.3390/ijms24054930The Proteome of Circulating Large Extracellular Vesicles in Diabetes and HypertensionAkram Abolbaghaei0Maddison Turner1Jean-François Thibodeau2Chet E. Holterman3Christopher R. J. Kennedy4Dylan Burger5Chronic Disease Program, Kidney Research Centre, Ottawa Hospital Research Institute, Ottawa, ON K1H 8M5, CanadaChronic Disease Program, Kidney Research Centre, Ottawa Hospital Research Institute, Ottawa, ON K1H 8M5, CanadaChronic Disease Program, Kidney Research Centre, Ottawa Hospital Research Institute, Ottawa, ON K1H 8M5, CanadaChronic Disease Program, Kidney Research Centre, Ottawa Hospital Research Institute, Ottawa, ON K1H 8M5, CanadaChronic Disease Program, Kidney Research Centre, Ottawa Hospital Research Institute, Ottawa, ON K1H 8M5, CanadaChronic Disease Program, Kidney Research Centre, Ottawa Hospital Research Institute, Ottawa, ON K1H 8M5, CanadaHypertension and diabetes induce vascular injury through processes that are not fully understood. Changes in extracellular vesicle (EV) composition could provide novel insights. Here, we examined the protein composition of circulating EVs from hypertensive, diabetic and healthy mice. EVs were isolated from transgenic mice overexpressing human renin in the liver (TtRhRen, hypertensive), OVE26 type 1 diabetic mice and wild-type (WT) mice. Protein content was analyzed using liquid chromatography–mass spectrometry. We identified 544 independent proteins, of which 408 were found in all groups, 34 were exclusive to WT, 16 were exclusive to OVE26 and 5 were exclusive to TTRhRen mice. Amongst the differentially expressed proteins, haptoglobin (HPT) was upregulated and ankyrin-1 (ANK1) was downregulated in OVE26 and TtRhRen mice compared with WT controls. Conversely, TSP4 and Co3A1 were upregulated and SAA4 was downregulated exclusively in diabetic mice; and PPN was upregulated and SPTB1 and SPTA1 were downregulated in hypertensive mice, compared to WT mice. Ingenuity pathway analysis identified enrichment in proteins associated with SNARE signaling, the complement system and NAD homeostasis in EVs from diabetic mice. Conversely, in EVs from hypertensive mice, there was enrichment in semaphroin and Rho signaling. Further analysis of these changes may improve understanding of vascular injury in hypertension and diabetes.https://www.mdpi.com/1422-0067/24/5/4930diabeteshypertensionvascularextracellular vesiclesmicroparticlesmicrovesicles |
| spellingShingle | Akram Abolbaghaei Maddison Turner Jean-François Thibodeau Chet E. Holterman Christopher R. J. Kennedy Dylan Burger The Proteome of Circulating Large Extracellular Vesicles in Diabetes and Hypertension International Journal of Molecular Sciences diabetes hypertension vascular extracellular vesicles microparticles microvesicles |
| title | The Proteome of Circulating Large Extracellular Vesicles in Diabetes and Hypertension |
| title_full | The Proteome of Circulating Large Extracellular Vesicles in Diabetes and Hypertension |
| title_fullStr | The Proteome of Circulating Large Extracellular Vesicles in Diabetes and Hypertension |
| title_full_unstemmed | The Proteome of Circulating Large Extracellular Vesicles in Diabetes and Hypertension |
| title_short | The Proteome of Circulating Large Extracellular Vesicles in Diabetes and Hypertension |
| title_sort | proteome of circulating large extracellular vesicles in diabetes and hypertension |
| topic | diabetes hypertension vascular extracellular vesicles microparticles microvesicles |
| url | https://www.mdpi.com/1422-0067/24/5/4930 |
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