Phoretic interactions and oscillations in active suspensions of growing Escherichia coli
Bioluminescence imaging experiments were carried out to characterize spatio-temporal patterns of bacterial self-organization in active suspensions (cultures) of bioluminescent Escherichia coli and its mutants. An analysis of the effects of mutations shows that spatio-temporal patterns formed in stan...
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| Format: | Article |
| Language: | English |
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The Royal Society
2018-01-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.180008 |
| _version_ | 1818114991230812160 |
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| author | Remigijus Šimkus Rita Meškienė Agota Aučynaitė Žilvinas Ledas Romas Baronas Rolandas Meškys |
| author_facet | Remigijus Šimkus Rita Meškienė Agota Aučynaitė Žilvinas Ledas Romas Baronas Rolandas Meškys |
| author_sort | Remigijus Šimkus |
| collection | DOAJ |
| description | Bioluminescence imaging experiments were carried out to characterize spatio-temporal patterns of bacterial self-organization in active suspensions (cultures) of bioluminescent Escherichia coli and its mutants. An analysis of the effects of mutations shows that spatio-temporal patterns formed in standard microtitre plates are not related to the chemotaxis system of bacteria. In fact, these patterns are strongly dependent on the properties of mutants that characterize them as self-phoretic (non-flagellar) swimmers. In particular, the observed patterns are essentially dependent on the efficiency of proton translocation across membranes and the smoothness of the cell surface. These characteristics can be associated, respectively, with the surface activity and the phoretic mobility of a colloidal swimmer. An analysis of the experimental data together with mathematical modelling of pattern formation suggests the following: (1) pattern-forming processes can be described by Keller–Segel-type models of chemotaxis with logistic cell kinetics; (2) active cells can be seen as biochemical oscillators that exhibit phoretic drift and alignment; and (3) the spatio-temporal patterns in a suspension of growing E. coli form due to phoretic interactions between oscillating cells of high metabolic activity. |
| first_indexed | 2024-12-11T03:59:31Z |
| format | Article |
| id | doaj.art-000fcb3762f143f3b79de9930f464163 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-11T03:59:31Z |
| publishDate | 2018-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-000fcb3762f143f3b79de9930f4641632022-12-22T01:21:41ZengThe Royal SocietyRoyal Society Open Science2054-57032018-01-015510.1098/rsos.180008180008Phoretic interactions and oscillations in active suspensions of growing Escherichia coliRemigijus ŠimkusRita MeškienėAgota AučynaitėŽilvinas LedasRomas BaronasRolandas MeškysBioluminescence imaging experiments were carried out to characterize spatio-temporal patterns of bacterial self-organization in active suspensions (cultures) of bioluminescent Escherichia coli and its mutants. An analysis of the effects of mutations shows that spatio-temporal patterns formed in standard microtitre plates are not related to the chemotaxis system of bacteria. In fact, these patterns are strongly dependent on the properties of mutants that characterize them as self-phoretic (non-flagellar) swimmers. In particular, the observed patterns are essentially dependent on the efficiency of proton translocation across membranes and the smoothness of the cell surface. These characteristics can be associated, respectively, with the surface activity and the phoretic mobility of a colloidal swimmer. An analysis of the experimental data together with mathematical modelling of pattern formation suggests the following: (1) pattern-forming processes can be described by Keller–Segel-type models of chemotaxis with logistic cell kinetics; (2) active cells can be seen as biochemical oscillators that exhibit phoretic drift and alignment; and (3) the spatio-temporal patterns in a suspension of growing E. coli form due to phoretic interactions between oscillating cells of high metabolic activity.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.180008bacterial growthbacterial chemotaxisbiochemical oscillationsjanus particlesself-phoresis |
| spellingShingle | Remigijus Šimkus Rita Meškienė Agota Aučynaitė Žilvinas Ledas Romas Baronas Rolandas Meškys Phoretic interactions and oscillations in active suspensions of growing Escherichia coli Royal Society Open Science bacterial growth bacterial chemotaxis biochemical oscillations janus particles self-phoresis |
| title | Phoretic interactions and oscillations in active suspensions of growing Escherichia coli |
| title_full | Phoretic interactions and oscillations in active suspensions of growing Escherichia coli |
| title_fullStr | Phoretic interactions and oscillations in active suspensions of growing Escherichia coli |
| title_full_unstemmed | Phoretic interactions and oscillations in active suspensions of growing Escherichia coli |
| title_short | Phoretic interactions and oscillations in active suspensions of growing Escherichia coli |
| title_sort | phoretic interactions and oscillations in active suspensions of growing escherichia coli |
| topic | bacterial growth bacterial chemotaxis biochemical oscillations janus particles self-phoresis |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.180008 |
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