Generation of an erythroid progenitor-derived iPSC line, VRISGi002-A, from a healthy 27-year-old Vietnamese donor under a feeder-free system
We have established the footprint-free Vietnamese human induced pluripotent stem cell (hiPSC) line, VRISGi002-A, from CD71 + CD235a + erythroid progenitor cells (EPCs) of a 27-year-old healthy donor. The EPCs were enriched from isolated peripheral blood and reprogrammed using Sendai viruses which ca...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Stem Cell Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122001738 |
| _version_ | 1818552428025348096 |
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| author | Thi-Hong Nhung Nguyen Thi-Tuyet Trinh Tran Thi-Hoa Luong Xuan-Hung Nguyen |
| author_facet | Thi-Hong Nhung Nguyen Thi-Tuyet Trinh Tran Thi-Hoa Luong Xuan-Hung Nguyen |
| author_sort | Thi-Hong Nhung Nguyen |
| collection | DOAJ |
| description | We have established the footprint-free Vietnamese human induced pluripotent stem cell (hiPSC) line, VRISGi002-A, from CD71 + CD235a + erythroid progenitor cells (EPCs) of a 27-year-old healthy donor. The EPCs were enriched from isolated peripheral blood and reprogrammed using Sendai viruses which carried the reprogramming factors c-MYC, SOX2, KLF4, and OCT4 under a feeder-free culture system. The established VRISGi002-A cell line expressed typical pluripotency markers, displayed a normal karyotype, and demonstrated the potential to differentiate into the three germ layers. This hiPSC line could serve as a Vietnamese healthy control model for physiological processes and drug screening. |
| first_indexed | 2024-12-12T09:13:03Z |
| format | Article |
| id | doaj.art-00137c159cd14d37985454337789d63a |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-12T09:13:03Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-00137c159cd14d37985454337789d63a2022-12-22T00:29:27ZengElsevierStem Cell Research1873-50612022-07-0162102824Generation of an erythroid progenitor-derived iPSC line, VRISGi002-A, from a healthy 27-year-old Vietnamese donor under a feeder-free systemThi-Hong Nhung Nguyen0Thi-Tuyet Trinh Tran1Thi-Hoa Luong2Xuan-Hung Nguyen3Center of Applied Sciences, Regenerative Medicine and Advance Technologies (CARA), Vinmec Healthcare System, Hanoi, Viet NamCenter of Applied Sciences, Regenerative Medicine and Advance Technologies (CARA), Vinmec Healthcare System, Hanoi, Viet NamCenter of Applied Sciences, Regenerative Medicine and Advance Technologies (CARA), Vinmec Healthcare System, Hanoi, Viet Nam; VNU, University of Science, Hanoi, Viet NamCenter of Applied Sciences, Regenerative Medicine and Advance Technologies (CARA), Vinmec Healthcare System, Hanoi, Viet Nam; College of Health Sciences, VinUniversity, Hanoi, Viet Nam; Corresponding author at: Center of Applied Sciences, Regenerative Medicine and Advance Technologies (CARA), Vinmec Healthcare System, Hanoi, Viet Nam.We have established the footprint-free Vietnamese human induced pluripotent stem cell (hiPSC) line, VRISGi002-A, from CD71 + CD235a + erythroid progenitor cells (EPCs) of a 27-year-old healthy donor. The EPCs were enriched from isolated peripheral blood and reprogrammed using Sendai viruses which carried the reprogramming factors c-MYC, SOX2, KLF4, and OCT4 under a feeder-free culture system. The established VRISGi002-A cell line expressed typical pluripotency markers, displayed a normal karyotype, and demonstrated the potential to differentiate into the three germ layers. This hiPSC line could serve as a Vietnamese healthy control model for physiological processes and drug screening.http://www.sciencedirect.com/science/article/pii/S1873506122001738Erythroid progenitorVietnamese iPSC lineFeeder-freeSendai virus |
| spellingShingle | Thi-Hong Nhung Nguyen Thi-Tuyet Trinh Tran Thi-Hoa Luong Xuan-Hung Nguyen Generation of an erythroid progenitor-derived iPSC line, VRISGi002-A, from a healthy 27-year-old Vietnamese donor under a feeder-free system Stem Cell Research Erythroid progenitor Vietnamese iPSC line Feeder-free Sendai virus |
| title | Generation of an erythroid progenitor-derived iPSC line, VRISGi002-A, from a healthy 27-year-old Vietnamese donor under a feeder-free system |
| title_full | Generation of an erythroid progenitor-derived iPSC line, VRISGi002-A, from a healthy 27-year-old Vietnamese donor under a feeder-free system |
| title_fullStr | Generation of an erythroid progenitor-derived iPSC line, VRISGi002-A, from a healthy 27-year-old Vietnamese donor under a feeder-free system |
| title_full_unstemmed | Generation of an erythroid progenitor-derived iPSC line, VRISGi002-A, from a healthy 27-year-old Vietnamese donor under a feeder-free system |
| title_short | Generation of an erythroid progenitor-derived iPSC line, VRISGi002-A, from a healthy 27-year-old Vietnamese donor under a feeder-free system |
| title_sort | generation of an erythroid progenitor derived ipsc line vrisgi002 a from a healthy 27 year old vietnamese donor under a feeder free system |
| topic | Erythroid progenitor Vietnamese iPSC line Feeder-free Sendai virus |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122001738 |
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