Structured illumination microscopy and its new developments
Optical microscopy allows us to observe the biological structures and processes within living cells. However, the spatial resolution of the optical microscopy is limited to about half of the wavelength by the light diffraction. Structured illumination microscopy (SIM), a type of new emerging super-r...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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World Scientific Publishing
2016-05-01
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| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | http://www.worldscientific.com/doi/pdf/10.1142/S179354581630010X |
| _version_ | 1828724791327064064 |
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| author | Jianling Chen Caimin Qiu Minghai You Xiaogang Chen Hongqin Yang Shusen Xie |
| author_facet | Jianling Chen Caimin Qiu Minghai You Xiaogang Chen Hongqin Yang Shusen Xie |
| author_sort | Jianling Chen |
| collection | DOAJ |
| description | Optical microscopy allows us to observe the biological structures and processes within living cells. However, the spatial resolution of the optical microscopy is limited to about half of the wavelength by the light diffraction. Structured illumination microscopy (SIM), a type of new emerging super-resolution microscopy, doubles the spatial resolution by illuminating the specimen with a patterned light, and the sample and light source requirements of SIM are not as strict as the other super-resolution microscopy. In addition, SIM is easier to combine with the other imaging techniques to improve their imaging resolution, leading to the developments of diverse types of SIM. SIM has great potential to meet the various requirements of living cells imaging. Here, we review the recent developments of SIM and its combination with other imaging techniques. |
| first_indexed | 2024-04-12T13:14:47Z |
| format | Article |
| id | doaj.art-0014cad127cb44558b5815e9e80cc027 |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-04-12T13:14:47Z |
| publishDate | 2016-05-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-0014cad127cb44558b5815e9e80cc0272022-12-22T03:31:43ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052016-05-01931630010-11630010-810.1142/S179354581630010X10.1142/S179354581630010XStructured illumination microscopy and its new developmentsJianling Chen0Caimin Qiu1Minghai You2Xiaogang Chen3Hongqin Yang4Shusen Xie5Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou 350007, P. R. ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou 350007, P. R. ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou 350007, P. R. ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou 350007, P. R. ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou 350007, P. R. ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou 350007, P. R. ChinaOptical microscopy allows us to observe the biological structures and processes within living cells. However, the spatial resolution of the optical microscopy is limited to about half of the wavelength by the light diffraction. Structured illumination microscopy (SIM), a type of new emerging super-resolution microscopy, doubles the spatial resolution by illuminating the specimen with a patterned light, and the sample and light source requirements of SIM are not as strict as the other super-resolution microscopy. In addition, SIM is easier to combine with the other imaging techniques to improve their imaging resolution, leading to the developments of diverse types of SIM. SIM has great potential to meet the various requirements of living cells imaging. Here, we review the recent developments of SIM and its combination with other imaging techniques.http://www.worldscientific.com/doi/pdf/10.1142/S179354581630010XStructured illumination microscopydiffraction limitsuper-resolution |
| spellingShingle | Jianling Chen Caimin Qiu Minghai You Xiaogang Chen Hongqin Yang Shusen Xie Structured illumination microscopy and its new developments Journal of Innovative Optical Health Sciences Structured illumination microscopy diffraction limit super-resolution |
| title | Structured illumination microscopy and its new developments |
| title_full | Structured illumination microscopy and its new developments |
| title_fullStr | Structured illumination microscopy and its new developments |
| title_full_unstemmed | Structured illumination microscopy and its new developments |
| title_short | Structured illumination microscopy and its new developments |
| title_sort | structured illumination microscopy and its new developments |
| topic | Structured illumination microscopy diffraction limit super-resolution |
| url | http://www.worldscientific.com/doi/pdf/10.1142/S179354581630010X |
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