Extract of <i>Aster koraiensis</i> Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action
<i>Aster koraiensis</i> Nakai (AK) leaf reportedly ameliorates health problems, such as diabetes. However, the effects of AK on cognitive dysfunction or memory impairment remain unclear. This study investigated whether AK leaf extract could attenuate cognitive impairment. We found that A...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/6/5765 |
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| author | Seung-Eun Lee Saetbyeol Park Gwi Yeong Jang Jeonghoon Lee Minho Moon Yun-Jeong Ji Ji Wook Jung Yunkwon Nam Soo Jung Shin Yunji Lee Jehun Choi Dong Hwi Kim |
| author_facet | Seung-Eun Lee Saetbyeol Park Gwi Yeong Jang Jeonghoon Lee Minho Moon Yun-Jeong Ji Ji Wook Jung Yunkwon Nam Soo Jung Shin Yunji Lee Jehun Choi Dong Hwi Kim |
| author_sort | Seung-Eun Lee |
| collection | DOAJ |
| description | <i>Aster koraiensis</i> Nakai (AK) leaf reportedly ameliorates health problems, such as diabetes. However, the effects of AK on cognitive dysfunction or memory impairment remain unclear. This study investigated whether AK leaf extract could attenuate cognitive impairment. We found that AK extract reduced the production of nitric oxide (NO), tumour necrosis factor (TNF)-α, phosphorylated-tau (p-tau), and the expression of inflammatory proteins in lipopolysaccharide- or amyloid-β-treated cells. AK extract exhibited inhibitory activity of control specific binding on N-methyl-D-aspartate (NMDA) receptors. Scopolamine-induced AD models were used chronically in rats and acutely in mice. Relative to negative controls (NC), hippocampal choline acetyltransferase (ChAT) and B-cell lymphoma 2 (Bcl2) activity was increased in rats chronically treated with scopolamine and fed an AK extract-containing diet. In the Y-maze test, spontaneous alterations were increased in the AK extract-fed groups compared to NC. Rats administered AK extract showed increased escape latency in the passive avoidance test. In the hippocampus of rats fed a high-AK extract diet (AKH), the expression of neuroactive ligand–receptor interaction-related genes, including Npy2r, Htr2c, and Rxfp1, was significantly altered. In the Morris water maze assay of mice acutely treated with scopolamine, the swimming times in the target quadrant of AK extract-treated groups increased significantly to the levels of the Donepezil and normal groups. We used Tg6799 Aβ-overexpressing 5XFAD transgenic mice to investigate Aβ accumulation in animals. In the AD model using 5XFAD, the administration of AK extract decreased amyloid-β (Aβ) accumulation and increased the number of NeuN antibody-reactive cells in the subiculum relative to the control group. In conclusion, AK extract ameliorated memory dysfunction by modulating ChAT activity and Bcl2-related anti-apoptotic pathways, affecting the expression of neuroactive ligand–receptor interaction-related genes and inhibiting Aβ accumulation. Therefore, AK extract could be a functional material improving cognition and memory. |
| first_indexed | 2024-03-11T06:24:38Z |
| format | Article |
| id | doaj.art-001878d7e84246b985d85e572ceb35cc |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:24:38Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-001878d7e84246b985d85e572ceb35cc2023-11-17T11:38:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246576510.3390/ijms24065765Extract of <i>Aster koraiensis</i> Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory ActionSeung-Eun Lee0Saetbyeol Park1Gwi Yeong Jang2Jeonghoon Lee3Minho Moon4Yun-Jeong Ji5Ji Wook Jung6Yunkwon Nam7Soo Jung Shin8Yunji Lee9Jehun Choi10Dong Hwi Kim11Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science (NIHHS), Eumseong 27709, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticultural & Herbal Science (NIHHS), Eumseong 27709, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticultural & Herbal Science (NIHHS), Eumseong 27709, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticultural & Herbal Science (NIHHS), Eumseong 27709, Republic of KoreaDepartment of Biochemistry, College of Medicine, Konyang University, Gwanjeodong-ro 158, Soe-gu, Daejeon 35365, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticultural & Herbal Science (NIHHS), Eumseong 27709, Republic of KoreaDivision of Biotechnology and Convergence, College of Cosmetics and Pharm, Daegu Haany University, Kyungsan 38610, Republic of KoreaDepartment of Biochemistry, College of Medicine, Konyang University, Gwanjeodong-ro 158, Soe-gu, Daejeon 35365, Republic of KoreaDepartment of Biochemistry, College of Medicine, Konyang University, Gwanjeodong-ro 158, Soe-gu, Daejeon 35365, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticultural & Herbal Science (NIHHS), Eumseong 27709, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticultural & Herbal Science (NIHHS), Eumseong 27709, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticultural & Herbal Science (NIHHS), Eumseong 27709, Republic of Korea<i>Aster koraiensis</i> Nakai (AK) leaf reportedly ameliorates health problems, such as diabetes. However, the effects of AK on cognitive dysfunction or memory impairment remain unclear. This study investigated whether AK leaf extract could attenuate cognitive impairment. We found that AK extract reduced the production of nitric oxide (NO), tumour necrosis factor (TNF)-α, phosphorylated-tau (p-tau), and the expression of inflammatory proteins in lipopolysaccharide- or amyloid-β-treated cells. AK extract exhibited inhibitory activity of control specific binding on N-methyl-D-aspartate (NMDA) receptors. Scopolamine-induced AD models were used chronically in rats and acutely in mice. Relative to negative controls (NC), hippocampal choline acetyltransferase (ChAT) and B-cell lymphoma 2 (Bcl2) activity was increased in rats chronically treated with scopolamine and fed an AK extract-containing diet. In the Y-maze test, spontaneous alterations were increased in the AK extract-fed groups compared to NC. Rats administered AK extract showed increased escape latency in the passive avoidance test. In the hippocampus of rats fed a high-AK extract diet (AKH), the expression of neuroactive ligand–receptor interaction-related genes, including Npy2r, Htr2c, and Rxfp1, was significantly altered. In the Morris water maze assay of mice acutely treated with scopolamine, the swimming times in the target quadrant of AK extract-treated groups increased significantly to the levels of the Donepezil and normal groups. We used Tg6799 Aβ-overexpressing 5XFAD transgenic mice to investigate Aβ accumulation in animals. In the AD model using 5XFAD, the administration of AK extract decreased amyloid-β (Aβ) accumulation and increased the number of NeuN antibody-reactive cells in the subiculum relative to the control group. In conclusion, AK extract ameliorated memory dysfunction by modulating ChAT activity and Bcl2-related anti-apoptotic pathways, affecting the expression of neuroactive ligand–receptor interaction-related genes and inhibiting Aβ accumulation. Therefore, AK extract could be a functional material improving cognition and memory.https://www.mdpi.com/1422-0067/24/6/5765<i>Aster koraiensis</i> NakaimemorycognitionB-cell lymphoma 2choline acetyltransferaseamyloid |
| spellingShingle | Seung-Eun Lee Saetbyeol Park Gwi Yeong Jang Jeonghoon Lee Minho Moon Yun-Jeong Ji Ji Wook Jung Yunkwon Nam Soo Jung Shin Yunji Lee Jehun Choi Dong Hwi Kim Extract of <i>Aster koraiensis</i> Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action International Journal of Molecular Sciences <i>Aster koraiensis</i> Nakai memory cognition B-cell lymphoma 2 choline acetyltransferase amyloid |
| title | Extract of <i>Aster koraiensis</i> Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action |
| title_full | Extract of <i>Aster koraiensis</i> Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action |
| title_fullStr | Extract of <i>Aster koraiensis</i> Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action |
| title_full_unstemmed | Extract of <i>Aster koraiensis</i> Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action |
| title_short | Extract of <i>Aster koraiensis</i> Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action |
| title_sort | extract of i aster koraiensis i nakai leaf ameliorates memory dysfunction via anti inflammatory action |
| topic | <i>Aster koraiensis</i> Nakai memory cognition B-cell lymphoma 2 choline acetyltransferase amyloid |
| url | https://www.mdpi.com/1422-0067/24/6/5765 |
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