New Data on Vaccine Antigen Deficient Bordetella pertussis Isolates
Evolution of Bordetella pertussis is driven by natural and vaccine pressures. Isolates circulating in regions with high vaccination coverage present multiple allelic and antigenic variations as compared to isolates collected before introduction of vaccination. Furthermore, during the last epidemics...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-09-01
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| Series: | Vaccines |
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| Online Access: | http://www.mdpi.com/2076-393X/3/3/751 |
| _version_ | 1811302289454923776 |
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| author | Valérie Bouchez Nicolas Hegerle Francesco Strati Elisabeth Njamkepo Nicole Guiso |
| author_facet | Valérie Bouchez Nicolas Hegerle Francesco Strati Elisabeth Njamkepo Nicole Guiso |
| author_sort | Valérie Bouchez |
| collection | DOAJ |
| description | Evolution of Bordetella pertussis is driven by natural and vaccine pressures. Isolates circulating in regions with high vaccination coverage present multiple allelic and antigenic variations as compared to isolates collected before introduction of vaccination. Furthermore, during the last epidemics reported in regions using pertussis acellular vaccines, isolates deficient for vaccine antigens, such as pertactin (PRN), were reported to reach high proportions of circulating isolates. More sporadic filamentous hemagglutinin (FHA) or pertussis toxin (PT) deficient isolates were also collected. The whole genome of some recent French isolates, deficient or non-deficient in vaccine antigens, were analyzed. Transcription profiles of the expression of the main virulence factors were also compared. The invasive phenotype in an in vitro human tracheal epithelial (HTE) cell model of infection was evaluated. Our genomic analysis focused on SNPs related to virulence genes known to be more likely to present allelic polymorphism. Transcriptomic data indicated that isolates circulating since the introduction of pertussis vaccines present lower transcription levels of the main virulence genes than the isolates of the pre-vaccine era. Furthermore, isolates not producing FHA present significantly higher expression levels of the entire set of genes tested. Finally, we observed that recent isolates are more invasive in HTE cells when compared to the reference strain, but no multiplication occurs within cells. |
| first_indexed | 2024-04-13T07:25:19Z |
| format | Article |
| id | doaj.art-0019c3ab1d0e4ac4aaf9b6f854f5ee6f |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-04-13T07:25:19Z |
| publishDate | 2015-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-0019c3ab1d0e4ac4aaf9b6f854f5ee6f2022-12-22T02:56:29ZengMDPI AGVaccines2076-393X2015-09-013375177010.3390/vaccines3030751vaccines3030751New Data on Vaccine Antigen Deficient Bordetella pertussis IsolatesValérie Bouchez0Nicolas Hegerle1Francesco Strati2Elisabeth Njamkepo3Nicole Guiso4Molecular Prevention and Therapy of Human Diseases, Institut Pasteur, 25 rue du Dr Roux, Paris 75015, FranceMolecular Prevention and Therapy of Human Diseases, Institut Pasteur, 25 rue du Dr Roux, Paris 75015, FranceMolecular Prevention and Therapy of Human Diseases, Institut Pasteur, 25 rue du Dr Roux, Paris 75015, FranceMolecular Prevention and Therapy of Human Diseases, Institut Pasteur, 25 rue du Dr Roux, Paris 75015, FranceMolecular Prevention and Therapy of Human Diseases, Institut Pasteur, 25 rue du Dr Roux, Paris 75015, FranceEvolution of Bordetella pertussis is driven by natural and vaccine pressures. Isolates circulating in regions with high vaccination coverage present multiple allelic and antigenic variations as compared to isolates collected before introduction of vaccination. Furthermore, during the last epidemics reported in regions using pertussis acellular vaccines, isolates deficient for vaccine antigens, such as pertactin (PRN), were reported to reach high proportions of circulating isolates. More sporadic filamentous hemagglutinin (FHA) or pertussis toxin (PT) deficient isolates were also collected. The whole genome of some recent French isolates, deficient or non-deficient in vaccine antigens, were analyzed. Transcription profiles of the expression of the main virulence factors were also compared. The invasive phenotype in an in vitro human tracheal epithelial (HTE) cell model of infection was evaluated. Our genomic analysis focused on SNPs related to virulence genes known to be more likely to present allelic polymorphism. Transcriptomic data indicated that isolates circulating since the introduction of pertussis vaccines present lower transcription levels of the main virulence genes than the isolates of the pre-vaccine era. Furthermore, isolates not producing FHA present significantly higher expression levels of the entire set of genes tested. Finally, we observed that recent isolates are more invasive in HTE cells when compared to the reference strain, but no multiplication occurs within cells.http://www.mdpi.com/2076-393X/3/3/751Bordetella pertussispertactinfilamentous hemagglutininvaccine antigen production deficience |
| spellingShingle | Valérie Bouchez Nicolas Hegerle Francesco Strati Elisabeth Njamkepo Nicole Guiso New Data on Vaccine Antigen Deficient Bordetella pertussis Isolates Vaccines Bordetella pertussis pertactin filamentous hemagglutinin vaccine antigen production deficience |
| title | New Data on Vaccine Antigen Deficient Bordetella pertussis Isolates |
| title_full | New Data on Vaccine Antigen Deficient Bordetella pertussis Isolates |
| title_fullStr | New Data on Vaccine Antigen Deficient Bordetella pertussis Isolates |
| title_full_unstemmed | New Data on Vaccine Antigen Deficient Bordetella pertussis Isolates |
| title_short | New Data on Vaccine Antigen Deficient Bordetella pertussis Isolates |
| title_sort | new data on vaccine antigen deficient bordetella pertussis isolates |
| topic | Bordetella pertussis pertactin filamentous hemagglutinin vaccine antigen production deficience |
| url | http://www.mdpi.com/2076-393X/3/3/751 |
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