Immunogenic protein profiling of pathogenic Escherichia coli strains isolated from infants with diarrhea in Quetta Balochistan
Escherichia coli (E. coli) is a widespread bacterial species that comprise a broad variety of strains and can be highly pathogenic often. Diarrhea caused by E. coli pathotypes is a leading public health problem in underdeveloped countries such as Pakistan. Five different diarrheagenic E. coli pathot...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722000647 |
| _version_ | 1818288784179986432 |
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| author | Zil-e-Huma Abdul Malik Tareen Abdul Samad Muhammad Zahid Mustafa Maqsood Maryam Sabeena Rizwan Ali Akbar |
| author_facet | Zil-e-Huma Abdul Malik Tareen Abdul Samad Muhammad Zahid Mustafa Maqsood Maryam Sabeena Rizwan Ali Akbar |
| author_sort | Zil-e-Huma |
| collection | DOAJ |
| description | Escherichia coli (E. coli) is a widespread bacterial species that comprise a broad variety of strains and can be highly pathogenic often. Diarrhea caused by E. coli pathotypes is a leading public health problem in underdeveloped countries such as Pakistan. Five different diarrheagenic E. coli pathotypes (Enterohaemorrhagic, Enteropathogenic, Enteroaggregative, Enteroinvasive and Enterotoxigenic E. coli) were isolated from 200 samples of diarrheal children in Quetta, Pakistan. Total-cell protein profiles of all the strains isolated were resolved via SDS-Polyacrylamide Gel Electrophoresis (PAGE). More than 15 protein bands were found ranging from 15 kDa to 153 kDa in size. A dendrogram plot explained the relatedness of the pathotypes amongst them. The protein bands of 70 and 15 kDa, analyzed through GelAnalyzer 2010a© were found common among all the isolated pathotypes. Purified bands of these two proteins (70 and 15 kDa) from the gel were injected in experimental mice groups for immunization purposes. During immunogenicity tests, the mice that were immunized with 70 and 15 kDa proteins stayed alive for seven days despite subsequently being challenged with the E. coli pathotypes, while higher mortality 80% were observed in the non-immunized control group. Upon the postmortem it was revealed that all the vital organs were covered by the E. coli strains injected for challenge studies. The immunized mice and control mice sera subjected to the Agar Gel Immunodiffusion assay (AGID) test for antibody/antigen reaction were found positive for antibodies presence. The exploration of immunogenic proteins among all the prevalent pathotypes can be a breakthrough in the development of a combined, effective, and safe vaccine. Further investigation of these immunogenic proteins would be of great importance for future immunization endeavors. |
| first_indexed | 2024-12-13T02:01:53Z |
| format | Article |
| id | doaj.art-001b062eb18d4d7fa01951e742303a21 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-13T02:01:53Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-001b062eb18d4d7fa01951e742303a212022-12-22T00:03:15ZengElsevierJournal of King Saud University: Science1018-36472022-04-01343101883Immunogenic protein profiling of pathogenic Escherichia coli strains isolated from infants with diarrhea in Quetta Balochistan Zil-e-Huma0Abdul Malik Tareen1Abdul Samad2Muhammad Zahid Mustafa3Maqsood Maryam4Sabeena Rizwan5Ali Akbar6Department of Zoology, Sardar Bahadur Khan Women’s University, Quetta, PakistanDepartment of Microbiology, University of Balochistan Quetta, PakistanCentre of Advanced Studies in Vaccinology and Biotechnology, University of Balochistan, Quetta, PakistanCentre of Advanced Studies in Vaccinology and Biotechnology, University of Balochistan, Quetta, PakistanDepartment of Zoology, Sardar Bahadur Khan Women’s University, Quetta, PakistanDepartment of Chemistry, Sardar Bahadur Khan Women’s University, Quetta, PakistanDepartment of Microbiology, University of Balochistan Quetta, Pakistan; Corresponding author.Escherichia coli (E. coli) is a widespread bacterial species that comprise a broad variety of strains and can be highly pathogenic often. Diarrhea caused by E. coli pathotypes is a leading public health problem in underdeveloped countries such as Pakistan. Five different diarrheagenic E. coli pathotypes (Enterohaemorrhagic, Enteropathogenic, Enteroaggregative, Enteroinvasive and Enterotoxigenic E. coli) were isolated from 200 samples of diarrheal children in Quetta, Pakistan. Total-cell protein profiles of all the strains isolated were resolved via SDS-Polyacrylamide Gel Electrophoresis (PAGE). More than 15 protein bands were found ranging from 15 kDa to 153 kDa in size. A dendrogram plot explained the relatedness of the pathotypes amongst them. The protein bands of 70 and 15 kDa, analyzed through GelAnalyzer 2010a© were found common among all the isolated pathotypes. Purified bands of these two proteins (70 and 15 kDa) from the gel were injected in experimental mice groups for immunization purposes. During immunogenicity tests, the mice that were immunized with 70 and 15 kDa proteins stayed alive for seven days despite subsequently being challenged with the E. coli pathotypes, while higher mortality 80% were observed in the non-immunized control group. Upon the postmortem it was revealed that all the vital organs were covered by the E. coli strains injected for challenge studies. The immunized mice and control mice sera subjected to the Agar Gel Immunodiffusion assay (AGID) test for antibody/antigen reaction were found positive for antibodies presence. The exploration of immunogenic proteins among all the prevalent pathotypes can be a breakthrough in the development of a combined, effective, and safe vaccine. Further investigation of these immunogenic proteins would be of great importance for future immunization endeavors.http://www.sciencedirect.com/science/article/pii/S1018364722000647InfectionsBacteriaAntibodiesAntigenImmunity |
| spellingShingle | Zil-e-Huma Abdul Malik Tareen Abdul Samad Muhammad Zahid Mustafa Maqsood Maryam Sabeena Rizwan Ali Akbar Immunogenic protein profiling of pathogenic Escherichia coli strains isolated from infants with diarrhea in Quetta Balochistan Journal of King Saud University: Science Infections Bacteria Antibodies Antigen Immunity |
| title | Immunogenic protein profiling of pathogenic Escherichia coli strains isolated from infants with diarrhea in Quetta Balochistan |
| title_full | Immunogenic protein profiling of pathogenic Escherichia coli strains isolated from infants with diarrhea in Quetta Balochistan |
| title_fullStr | Immunogenic protein profiling of pathogenic Escherichia coli strains isolated from infants with diarrhea in Quetta Balochistan |
| title_full_unstemmed | Immunogenic protein profiling of pathogenic Escherichia coli strains isolated from infants with diarrhea in Quetta Balochistan |
| title_short | Immunogenic protein profiling of pathogenic Escherichia coli strains isolated from infants with diarrhea in Quetta Balochistan |
| title_sort | immunogenic protein profiling of pathogenic escherichia coli strains isolated from infants with diarrhea in quetta balochistan |
| topic | Infections Bacteria Antibodies Antigen Immunity |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722000647 |
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