How to store plant tissues in the absence of liquid nitrogen? Ethanol preserves the RNA integrity of <em>Cannabis sativa</em> stem tissues
The preservation of intact RNA is a limiting step when gene expression profiling is performed using field-collected plant material. The use of liquid nitrogen ensures the optimal preservation of RNA, however it is not always practical, especially if the plant material has to be sampled in remote loc...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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AIMS Press
2016-09-01
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| Series: | AIMS Molecular Science |
| Subjects: | |
| Online Access: | http://www.aimspress.com/Molecular/article/1002/fulltext.html |
| _version_ | 1818014735373697024 |
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| author | Lauralie Mangeot-Peter Sylvain Legay Jean-Francois Hausman Gea Guerriero |
| author_facet | Lauralie Mangeot-Peter Sylvain Legay Jean-Francois Hausman Gea Guerriero |
| author_sort | Lauralie Mangeot-Peter |
| collection | DOAJ |
| description | The preservation of intact RNA is a limiting step when gene expression profiling is performed using field-collected plant material. The use of liquid nitrogen ensures the optimal preservation of RNA, however it is not always practical, especially if the plant material has to be sampled in remote locations. Ethanol is known to preserve DNA in plant tissues even after a long storage period and here its suitability to preserve the RNA of textile hemp cortical tissues was tested. Hemp (<em>Cannabis sativa</em> L.) is an economically important fibre crop because it supplies cellulosic bast fibres used in different industrial sectors. In this study we demonstrate the suitability of ethanol for RNA preservation by analyzing tissues stored at 4 °C for 1, 2, 4 and 8 days. We show that in all the cases the extracted RNA is intact. We finally analyze hemp stem tissues stored in ethanol for 1 month and demonstrate the preservation of the tissue structure, particularly of bast fibres. |
| first_indexed | 2024-04-14T06:48:07Z |
| format | Article |
| id | doaj.art-002003b8682e4463b479fdd9c85fbaa3 |
| institution | Directory Open Access Journal |
| issn | 2372-0301 |
| language | English |
| last_indexed | 2024-04-14T06:48:07Z |
| publishDate | 2016-09-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Molecular Science |
| spelling | doaj.art-002003b8682e4463b479fdd9c85fbaa32022-12-22T02:07:06ZengAIMS PressAIMS Molecular Science2372-03012016-09-013456056610.3934/molsci.2016.4.560molsci-03-00560How to store plant tissues in the absence of liquid nitrogen? Ethanol preserves the RNA integrity of <em>Cannabis sativa</em> stem tissuesLauralie Mangeot-Peter0Sylvain Legay1Jean-Francois Hausman2Gea Guerriero3Environmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), L-4362 Esch/Alzette, LuxembourgEnvironmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), L-4362 Esch/Alzette, LuxembourgEnvironmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), L-4362 Esch/Alzette, LuxembourgEnvironmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), L-4362 Esch/Alzette, LuxembourgThe preservation of intact RNA is a limiting step when gene expression profiling is performed using field-collected plant material. The use of liquid nitrogen ensures the optimal preservation of RNA, however it is not always practical, especially if the plant material has to be sampled in remote locations. Ethanol is known to preserve DNA in plant tissues even after a long storage period and here its suitability to preserve the RNA of textile hemp cortical tissues was tested. Hemp (<em>Cannabis sativa</em> L.) is an economically important fibre crop because it supplies cellulosic bast fibres used in different industrial sectors. In this study we demonstrate the suitability of ethanol for RNA preservation by analyzing tissues stored at 4 °C for 1, 2, 4 and 8 days. We show that in all the cases the extracted RNA is intact. We finally analyze hemp stem tissues stored in ethanol for 1 month and demonstrate the preservation of the tissue structure, particularly of bast fibres.http://www.aimspress.com/Molecular/article/1002/fulltext.htmlRNA preservationethanolbast fibres<em>Cannabis sativa</em>integrity |
| spellingShingle | Lauralie Mangeot-Peter Sylvain Legay Jean-Francois Hausman Gea Guerriero How to store plant tissues in the absence of liquid nitrogen? Ethanol preserves the RNA integrity of <em>Cannabis sativa</em> stem tissues AIMS Molecular Science RNA preservation ethanol bast fibres <em>Cannabis sativa</em> integrity |
| title | How to store plant tissues in the absence of liquid nitrogen? Ethanol preserves the RNA integrity of <em>Cannabis sativa</em> stem tissues |
| title_full | How to store plant tissues in the absence of liquid nitrogen? Ethanol preserves the RNA integrity of <em>Cannabis sativa</em> stem tissues |
| title_fullStr | How to store plant tissues in the absence of liquid nitrogen? Ethanol preserves the RNA integrity of <em>Cannabis sativa</em> stem tissues |
| title_full_unstemmed | How to store plant tissues in the absence of liquid nitrogen? Ethanol preserves the RNA integrity of <em>Cannabis sativa</em> stem tissues |
| title_short | How to store plant tissues in the absence of liquid nitrogen? Ethanol preserves the RNA integrity of <em>Cannabis sativa</em> stem tissues |
| title_sort | how to store plant tissues in the absence of liquid nitrogen ethanol preserves the rna integrity of em cannabis sativa em stem tissues |
| topic | RNA preservation ethanol bast fibres <em>Cannabis sativa</em> integrity |
| url | http://www.aimspress.com/Molecular/article/1002/fulltext.html |
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