Protective Effects of Alpha-Lipoic Acid on Oleic Acid-Induced Acute Lung Injury in Rats
Background: Oxidative stress is believed to be an important factor in the pathogenesis of acute lung injury (ALI). Aims: The aim of this study was to investigate the possible protective role of alpha-lipoic acid (α-LA) on oleic acid (OA)-induced ALI in rats. Study Design: Animal experiment....
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2013-09-01
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| Series: | Balkan Medical Journal |
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| Online Access: | http://balkanmedicaljournal.org/text.php?lang=en&id=370 |
| _version_ | 1828019198020812800 |
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| author | Funda Gülcü Bulmuş Mehmet Ferit Gürsu Mehmet Hamdi Muz İhsan Yaman Özgür Bulmuş Fatih Sakin |
| author_facet | Funda Gülcü Bulmuş Mehmet Ferit Gürsu Mehmet Hamdi Muz İhsan Yaman Özgür Bulmuş Fatih Sakin |
| author_sort | Funda Gülcü Bulmuş |
| collection | DOAJ |
| description | Background: Oxidative stress is believed to be an important factor in the pathogenesis of acute lung injury (ALI).
Aims: The aim of this study was to investigate the possible protective role of alpha-lipoic acid (α-LA) on oleic acid (OA)-induced ALI in rats.
Study Design: Animal experiment.
Methods: A total of thirty-five rats were divided into five groups in the study. Group 1 served as a control group. Rats in Group 2 (α-LA) were administered α-LA intraperitoneally at a dose of 100 mg/kg body weight (BW). Rats in Group 3 (OA) were administered OA intravenously at a dose of 100 mg/kg BW. In Group 4 (pre-OA-α-LA), α-LA was given 15 minutes prior to OA infusion, and in Group 5 (post-OA-α-LA), α-LA was given two hours after OA infusion. Four hours after the OA infusion, rats were decapitated. Blood samples were collected to measure serum levels of malondialdehyde (MDA) and glutathione (GSH), and the levels of activity for superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). Lung tissue samples were taken for histopathological examination.
Results: Exposure to OA resulted in increases in serum MDA levels (p<0.001), as well as histopathological lesions in lung tissue, and decreases in CAT (p<0.05), GSH-Px (p<0.05) activities and GSH (p<0.05) levels. On the other hand, MDA levels were decreased significantly (p<0.001), while CAT (p<0.05), GSH-Px (p<0.01) activities and GSH (p<0.05) levels were increased significantly in the pre-OA-α-LA group compared with the OA group.
Conclusion: α-LA was found to lessen oxidative stress and to have positive effects on antioxidants in cases of OA-induced ALI. In conclusion, α-LA appears to have protective effects against ALI and potential for the prevention of ALI. |
| first_indexed | 2024-04-10T11:15:13Z |
| format | Article |
| id | doaj.art-00205636355b4585ac3fa0caf5de46f0 |
| institution | Directory Open Access Journal |
| issn | 2146-3123 2146-3131 |
| language | English |
| last_indexed | 2024-04-10T11:15:13Z |
| publishDate | 2013-09-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Balkan Medical Journal |
| spelling | doaj.art-00205636355b4585ac3fa0caf5de46f02023-02-15T16:18:53ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312013-09-0130330931410.5152/balkanmedj.2013.8426Protective Effects of Alpha-Lipoic Acid on Oleic Acid-Induced Acute Lung Injury in RatsFunda Gülcü Bulmuş0Mehmet Ferit Gürsu1Mehmet Hamdi Muz2İhsan Yaman3Özgür Bulmuş4Fatih Sakin5Vocational School of Health Services, Fırat University, Elazığ, TurkeyDepartment of Biochemistry, Fırat University Faculty of Medicine, Elazığ, TurkeyDepartment of Pulmonary Diseases, Fırat University Faculty of Medicine, Elazığ, TurkeySivrice Vocational School, Fırat University, Elazığ, Turkey Veterinary Control Institute, Ministry of Agriculture, Elazığ, TurkeyDepartment of Pharmacology and Toxicology, Mustafa Kemal University Faculty of Veterinary Medicine, Hatay, TurkeyBackground: Oxidative stress is believed to be an important factor in the pathogenesis of acute lung injury (ALI). Aims: The aim of this study was to investigate the possible protective role of alpha-lipoic acid (α-LA) on oleic acid (OA)-induced ALI in rats. Study Design: Animal experiment. Methods: A total of thirty-five rats were divided into five groups in the study. Group 1 served as a control group. Rats in Group 2 (α-LA) were administered α-LA intraperitoneally at a dose of 100 mg/kg body weight (BW). Rats in Group 3 (OA) were administered OA intravenously at a dose of 100 mg/kg BW. In Group 4 (pre-OA-α-LA), α-LA was given 15 minutes prior to OA infusion, and in Group 5 (post-OA-α-LA), α-LA was given two hours after OA infusion. Four hours after the OA infusion, rats were decapitated. Blood samples were collected to measure serum levels of malondialdehyde (MDA) and glutathione (GSH), and the levels of activity for superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). Lung tissue samples were taken for histopathological examination. Results: Exposure to OA resulted in increases in serum MDA levels (p<0.001), as well as histopathological lesions in lung tissue, and decreases in CAT (p<0.05), GSH-Px (p<0.05) activities and GSH (p<0.05) levels. On the other hand, MDA levels were decreased significantly (p<0.001), while CAT (p<0.05), GSH-Px (p<0.01) activities and GSH (p<0.05) levels were increased significantly in the pre-OA-α-LA group compared with the OA group. Conclusion: α-LA was found to lessen oxidative stress and to have positive effects on antioxidants in cases of OA-induced ALI. In conclusion, α-LA appears to have protective effects against ALI and potential for the prevention of ALI.http://balkanmedicaljournal.org/text.php?lang=en&id=370Acute lung injuryoleic acidα-lipoic acidoxidative stress |
| spellingShingle | Funda Gülcü Bulmuş Mehmet Ferit Gürsu Mehmet Hamdi Muz İhsan Yaman Özgür Bulmuş Fatih Sakin Protective Effects of Alpha-Lipoic Acid on Oleic Acid-Induced Acute Lung Injury in Rats Balkan Medical Journal Acute lung injury oleic acid α-lipoic acid oxidative stress |
| title | Protective Effects of Alpha-Lipoic Acid on Oleic Acid-Induced Acute Lung Injury in Rats |
| title_full | Protective Effects of Alpha-Lipoic Acid on Oleic Acid-Induced Acute Lung Injury in Rats |
| title_fullStr | Protective Effects of Alpha-Lipoic Acid on Oleic Acid-Induced Acute Lung Injury in Rats |
| title_full_unstemmed | Protective Effects of Alpha-Lipoic Acid on Oleic Acid-Induced Acute Lung Injury in Rats |
| title_short | Protective Effects of Alpha-Lipoic Acid on Oleic Acid-Induced Acute Lung Injury in Rats |
| title_sort | protective effects of alpha lipoic acid on oleic acid induced acute lung injury in rats |
| topic | Acute lung injury oleic acid α-lipoic acid oxidative stress |
| url | http://balkanmedicaljournal.org/text.php?lang=en&id=370 |
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