Ameliorative Effect of Melatonin Versus the Passage of Time and Lipid Peroxidation on Sperm Motility in Asthenotratospermic Men
Background and Objective: Downscale outcome in assisted reproductive techniques (ART) is associated with lipid peroxidation. Melatonin may have beneficial effects against lipid peroxidation and oxidative stress. This work was aimed to evaluate the ameliorative capacity of melatonin versus the passag...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Golestan University of Medical Sciences
2021-03-01
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| Series: | Jorjani Biomedicine Journal |
| Subjects: | |
| Online Access: | http://goums.ac.ir/jorjanijournal/article-1-791-en.html |
| _version_ | 1818393660697346048 |
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| author | Ghadir Sohrabi Mahdi Malmir Ali Asghar Ghafarizade Tayebeh Faraji |
| author_facet | Ghadir Sohrabi Mahdi Malmir Ali Asghar Ghafarizade Tayebeh Faraji |
| author_sort | Ghadir Sohrabi |
| collection | DOAJ |
| description | Background and Objective: Downscale outcome in assisted reproductive techniques (ART) is associated with lipid peroxidation. Melatonin may have beneficial effects against lipid peroxidation and oxidative stress. This work was aimed to evaluate the ameliorative capacity of melatonin versus the passage of time and lipid peroxidation on sperm parameters from the men with ATS in the ART process.
Material and Methods: Semen samples were collected from 50 asthenoteratozoospermic (ATS) men. Samples were divided into control and melatonin group. The test group were incubated with 6 mM melatonin at 2, 4, 6, and 24 hours. Then total and progressive motility, membrane integrity, and Malondialdehyde levels (MDA) were evaluated. Statistical analysis was carried out by SPSS software (repeated-measures ANOVA).
Results: In the control group, total (p<0.01) and progressive (P<0.05) motility and also, membrane integrity (P<0.03) significantly decreased although, MDA levels of sperm significantly increased (*P<0.04). Moreover, in the melatonin group, the mentioned parameters significantly compensated compared to the control group (P<0.05).
Conclusion: In the outcome, melatonin in vitro treatment paves the way for motility and membrane integrity up-regulating in the ATS men. |
| first_indexed | 2024-12-14T05:48:51Z |
| format | Article |
| id | doaj.art-0020a8e50ea9424eb86b1ef09c832e31 |
| institution | Directory Open Access Journal |
| issn | 2645-3509 2645-3509 |
| language | English |
| last_indexed | 2024-12-14T05:48:51Z |
| publishDate | 2021-03-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Jorjani Biomedicine Journal |
| spelling | doaj.art-0020a8e50ea9424eb86b1ef09c832e312022-12-21T23:14:48ZengGolestan University of Medical SciencesJorjani Biomedicine Journal2645-35092645-35092021-03-01914454Ameliorative Effect of Melatonin Versus the Passage of Time and Lipid Peroxidation on Sperm Motility in Asthenotratospermic MenGhadir Sohrabi0Mahdi Malmir1Ali Asghar Ghafarizade2Tayebeh Faraji3 Tabriz University of Medical Sciences International Branch Aras Department of Midwifery, Tuyserkan Branch, Islamic Azad University, Tuyserkan, Iran Academic Center for Education, Culture and Research, Arak, Iran Academic Center for Education, Culture and Research, Arak, Iran Background and Objective: Downscale outcome in assisted reproductive techniques (ART) is associated with lipid peroxidation. Melatonin may have beneficial effects against lipid peroxidation and oxidative stress. This work was aimed to evaluate the ameliorative capacity of melatonin versus the passage of time and lipid peroxidation on sperm parameters from the men with ATS in the ART process. Material and Methods: Semen samples were collected from 50 asthenoteratozoospermic (ATS) men. Samples were divided into control and melatonin group. The test group were incubated with 6 mM melatonin at 2, 4, 6, and 24 hours. Then total and progressive motility, membrane integrity, and Malondialdehyde levels (MDA) were evaluated. Statistical analysis was carried out by SPSS software (repeated-measures ANOVA). Results: In the control group, total (p<0.01) and progressive (P<0.05) motility and also, membrane integrity (P<0.03) significantly decreased although, MDA levels of sperm significantly increased (*P<0.04). Moreover, in the melatonin group, the mentioned parameters significantly compensated compared to the control group (P<0.05). Conclusion: In the outcome, melatonin in vitro treatment paves the way for motility and membrane integrity up-regulating in the ATS men.http://goums.ac.ir/jorjanijournal/article-1-791-en.htmlmelatoninlipid peroxidationasthenospermia and teratozoospermiamembrane integrityart |
| spellingShingle | Ghadir Sohrabi Mahdi Malmir Ali Asghar Ghafarizade Tayebeh Faraji Ameliorative Effect of Melatonin Versus the Passage of Time and Lipid Peroxidation on Sperm Motility in Asthenotratospermic Men Jorjani Biomedicine Journal melatonin lipid peroxidation asthenospermia and teratozoospermia membrane integrity art |
| title | Ameliorative Effect of Melatonin Versus the Passage of Time and Lipid Peroxidation on Sperm Motility in Asthenotratospermic Men |
| title_full | Ameliorative Effect of Melatonin Versus the Passage of Time and Lipid Peroxidation on Sperm Motility in Asthenotratospermic Men |
| title_fullStr | Ameliorative Effect of Melatonin Versus the Passage of Time and Lipid Peroxidation on Sperm Motility in Asthenotratospermic Men |
| title_full_unstemmed | Ameliorative Effect of Melatonin Versus the Passage of Time and Lipid Peroxidation on Sperm Motility in Asthenotratospermic Men |
| title_short | Ameliorative Effect of Melatonin Versus the Passage of Time and Lipid Peroxidation on Sperm Motility in Asthenotratospermic Men |
| title_sort | ameliorative effect of melatonin versus the passage of time and lipid peroxidation on sperm motility in asthenotratospermic men |
| topic | melatonin lipid peroxidation asthenospermia and teratozoospermia membrane integrity art |
| url | http://goums.ac.ir/jorjanijournal/article-1-791-en.html |
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