Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid Raft
The influenza glycoproteins, hemagglutinin (HA) and neuraminidase (NA), which are associated with the lipid raft, have the potential to initiate virion budding. However, the role of these viral proteins in infectious virion assembly is still unclear. In addition, it is not known how the viral ribonu...
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| Format: | Article |
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MDPI AG
2016-09-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/8/9/249 |
| _version_ | 1818513868124585984 |
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| author | Naoki Takizawa Fumitaka Momose Yuko Morikawa Akio Nomoto |
| author_facet | Naoki Takizawa Fumitaka Momose Yuko Morikawa Akio Nomoto |
| author_sort | Naoki Takizawa |
| collection | DOAJ |
| description | The influenza glycoproteins, hemagglutinin (HA) and neuraminidase (NA), which are associated with the lipid raft, have the potential to initiate virion budding. However, the role of these viral proteins in infectious virion assembly is still unclear. In addition, it is not known how the viral ribonucleoprotein complex (vRNP) is tethered to the budding site. Here, we show that HA is necessary for the efficient progeny virion production and vRNP packaging in the virion. We also found that the level of HA does not affect the bundling of the eight vRNP segments, despite reduced virion production. Detergent solubilization and a subsequent membrane flotation analysis indicated that the accumulation of nucleoprotein, viral polymerases, NA, and matrix protein 1 (M1) in the lipid raft fraction was delayed without HA. Based on our results, we inferred that HA plays a role in the accumulation of viral components, including bundled vRNPs, at the lipid raft. |
| first_indexed | 2024-12-11T00:07:27Z |
| format | Article |
| id | doaj.art-0022bf5956554b8f9c2e7acdbadebf22 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-11T00:07:27Z |
| publishDate | 2016-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-0022bf5956554b8f9c2e7acdbadebf222022-12-22T01:28:16ZengMDPI AGViruses1999-49152016-09-018924910.3390/v8090249v8090249Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid RaftNaoki Takizawa0Fumitaka Momose1Yuko Morikawa2Akio Nomoto3Laboratory of Virology, Institute of Microbial Chemistry (BIKAKEN), 3-14-23 Kamiosaki, Shinagawa-ku, Tokyo 141-0021, JapanKitasato Institute for Life Sciences and Graduate School for Infection Control, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, JapanKitasato Institute for Life Sciences and Graduate School for Infection Control, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, JapanLaboratory of Virology, Institute of Microbial Chemistry (BIKAKEN), 3-14-23 Kamiosaki, Shinagawa-ku, Tokyo 141-0021, JapanThe influenza glycoproteins, hemagglutinin (HA) and neuraminidase (NA), which are associated with the lipid raft, have the potential to initiate virion budding. However, the role of these viral proteins in infectious virion assembly is still unclear. In addition, it is not known how the viral ribonucleoprotein complex (vRNP) is tethered to the budding site. Here, we show that HA is necessary for the efficient progeny virion production and vRNP packaging in the virion. We also found that the level of HA does not affect the bundling of the eight vRNP segments, despite reduced virion production. Detergent solubilization and a subsequent membrane flotation analysis indicated that the accumulation of nucleoprotein, viral polymerases, NA, and matrix protein 1 (M1) in the lipid raft fraction was delayed without HA. Based on our results, we inferred that HA plays a role in the accumulation of viral components, including bundled vRNPs, at the lipid raft.http://www.mdpi.com/1999-4915/8/9/249influenza viruslipid raftvirus buddingviral genome packaginghemagglutinin |
| spellingShingle | Naoki Takizawa Fumitaka Momose Yuko Morikawa Akio Nomoto Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid Raft Viruses influenza virus lipid raft virus budding viral genome packaging hemagglutinin |
| title | Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid Raft |
| title_full | Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid Raft |
| title_fullStr | Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid Raft |
| title_full_unstemmed | Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid Raft |
| title_short | Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid Raft |
| title_sort | influenza a virus hemagglutinin is required for the assembly of viral components including bundled vrnps at the lipid raft |
| topic | influenza virus lipid raft virus budding viral genome packaging hemagglutinin |
| url | http://www.mdpi.com/1999-4915/8/9/249 |
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