A MADS-Box Gene <i>CiMADS43</i> Is Involved in Citrus Flowering and Leaf Development through Interaction with CiAGL9
MADS-box genes are involved in various developmental processes including vegetative development, flower architecture, flowering, pollen formation, seed and fruit development. However, the function of most MADS-box genes and their regulation mechanism are still unclear in woody plants compared with m...
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MDPI AG
2021-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/10/5205 |
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| author | Li-Xia Ye Jin-Xia Zhang Xiao-Jin Hou Mei-Qi Qiu Wen-Feng Wang Jin-Xin Zhang Chun-Gen Hu Jin-Zhi Zhang |
| author_facet | Li-Xia Ye Jin-Xia Zhang Xiao-Jin Hou Mei-Qi Qiu Wen-Feng Wang Jin-Xin Zhang Chun-Gen Hu Jin-Zhi Zhang |
| author_sort | Li-Xia Ye |
| collection | DOAJ |
| description | MADS-box genes are involved in various developmental processes including vegetative development, flower architecture, flowering, pollen formation, seed and fruit development. However, the function of most MADS-box genes and their regulation mechanism are still unclear in woody plants compared with model plants. In this study, a MADS-box gene (<i>CiMADS43</i>) was identified in citrus. Phylogenetic and sequence analysis showed that <i>CiMADS43</i> is a GOA-like Bsister MADS-box gene. It was localized in the nucleus and as a transcriptional activator. Overexpression of <i>CiMADS43</i> promoted early flowering and leaves curling in transgenic <i>Arabidopsis</i>. Besides, overexpression or knockout of <i>CiMADS43</i> also showed leaf curl phenotype in citrus similar to that of <i>CiMADS43</i> overexpressed in <i>Arabidopsis</i>. Protein–protein interaction found that a SEPALLATA (SEP)-like protein (CiAGL9) interacted with CiMADS43 protein. Interestingly, <i>CiAGL9</i> also can bind to the <i>CiMADS43</i> promoter and promote its transcription. Expression analysis also showed that these two genes were closely related to seasonal flowering and the development of the leaf in citrus. Our findings revealed the multifunctional roles of <i>CiMADS43</i> in the vegetative and reproductive development of citrus. These results will facilitate our understanding of the evolution and molecular mechanisms of MADS-box genes in citrus. |
| first_indexed | 2024-03-10T11:24:28Z |
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| language | English |
| last_indexed | 2024-03-10T11:24:28Z |
| publishDate | 2021-05-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0023662171a54b2bb191b2003c51f3242023-11-21T19:45:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-012210520510.3390/ijms22105205A MADS-Box Gene <i>CiMADS43</i> Is Involved in Citrus Flowering and Leaf Development through Interaction with CiAGL9Li-Xia Ye0Jin-Xia Zhang1Xiao-Jin Hou2Mei-Qi Qiu3Wen-Feng Wang4Jin-Xin Zhang5Chun-Gen Hu6Jin-Zhi Zhang7Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, ChinaMADS-box genes are involved in various developmental processes including vegetative development, flower architecture, flowering, pollen formation, seed and fruit development. However, the function of most MADS-box genes and their regulation mechanism are still unclear in woody plants compared with model plants. In this study, a MADS-box gene (<i>CiMADS43</i>) was identified in citrus. Phylogenetic and sequence analysis showed that <i>CiMADS43</i> is a GOA-like Bsister MADS-box gene. It was localized in the nucleus and as a transcriptional activator. Overexpression of <i>CiMADS43</i> promoted early flowering and leaves curling in transgenic <i>Arabidopsis</i>. Besides, overexpression or knockout of <i>CiMADS43</i> also showed leaf curl phenotype in citrus similar to that of <i>CiMADS43</i> overexpressed in <i>Arabidopsis</i>. Protein–protein interaction found that a SEPALLATA (SEP)-like protein (CiAGL9) interacted with CiMADS43 protein. Interestingly, <i>CiAGL9</i> also can bind to the <i>CiMADS43</i> promoter and promote its transcription. Expression analysis also showed that these two genes were closely related to seasonal flowering and the development of the leaf in citrus. Our findings revealed the multifunctional roles of <i>CiMADS43</i> in the vegetative and reproductive development of citrus. These results will facilitate our understanding of the evolution and molecular mechanisms of MADS-box genes in citrus.https://www.mdpi.com/1422-0067/22/10/5205citrus<i>CiMADS43</i><i>CiAGL9</i>floweringleaf |
| spellingShingle | Li-Xia Ye Jin-Xia Zhang Xiao-Jin Hou Mei-Qi Qiu Wen-Feng Wang Jin-Xin Zhang Chun-Gen Hu Jin-Zhi Zhang A MADS-Box Gene <i>CiMADS43</i> Is Involved in Citrus Flowering and Leaf Development through Interaction with CiAGL9 International Journal of Molecular Sciences citrus <i>CiMADS43</i> <i>CiAGL9</i> flowering leaf |
| title | A MADS-Box Gene <i>CiMADS43</i> Is Involved in Citrus Flowering and Leaf Development through Interaction with CiAGL9 |
| title_full | A MADS-Box Gene <i>CiMADS43</i> Is Involved in Citrus Flowering and Leaf Development through Interaction with CiAGL9 |
| title_fullStr | A MADS-Box Gene <i>CiMADS43</i> Is Involved in Citrus Flowering and Leaf Development through Interaction with CiAGL9 |
| title_full_unstemmed | A MADS-Box Gene <i>CiMADS43</i> Is Involved in Citrus Flowering and Leaf Development through Interaction with CiAGL9 |
| title_short | A MADS-Box Gene <i>CiMADS43</i> Is Involved in Citrus Flowering and Leaf Development through Interaction with CiAGL9 |
| title_sort | mads box gene i cimads43 i is involved in citrus flowering and leaf development through interaction with ciagl9 |
| topic | citrus <i>CiMADS43</i> <i>CiAGL9</i> flowering leaf |
| url | https://www.mdpi.com/1422-0067/22/10/5205 |
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