Effect of Sambucus ebulus extract on neural stem cell prolifration under oxidative stress condition
Background and Aim: Recently, several studies have indicated that the central nervous system has the capacity for endogenous repair. But, the proliferation capacity of endogenous neural stem cells (NSCs) isn’t sufficient for the treatment of neurodegenerative diseases. So, it sounds that stimulation...
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| Format: | Article |
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Kurdistan University of Medical Sciences
2018-08-01
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| Series: | مجله علمی دانشگاه علوم پزشکی کردستان |
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| Online Access: | http://sjku.muk.ac.ir/article-1-4264-en.html |
| _version_ | 1818370243264774144 |
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| author | Sara Haratizadeh Mohammad Ali Ebrahimzadeh Maryam Nazm Bojnordi Hatef Ghasemi Hamidabadi Alireza Abdanipour Javad Akhtari |
| author_facet | Sara Haratizadeh Mohammad Ali Ebrahimzadeh Maryam Nazm Bojnordi Hatef Ghasemi Hamidabadi Alireza Abdanipour Javad Akhtari |
| author_sort | Sara Haratizadeh |
| collection | DOAJ |
| description | Background and Aim: Recently, several studies have indicated that the central nervous system has the capacity for endogenous repair. But, the proliferation capacity of endogenous neural stem cells (NSCs) isn’t sufficient for the treatment of neurodegenerative diseases. So, it sounds that stimulation of endogenous NSC proliferation is essential for neuroregeneration. The aim of this study was to examine the effects of Sambucus ebulus extract on the proliferation of neonatal rat hippocampus-derived neural stem cells (NSCs) under oxidative stress condition induced by H2O2.
Material and Methods: The NSCs were isolated from neonatal rat hippocampus. To confirm neural characteristics of neural stem cells, the expression of neural-specific marker, Nestin was investigated by immunocytochemistry technique. 5×104 cells were cultured in every well of a 96 well plate and H2O2 was added to induce oxidative stress condition. Then NSCs were exposed to 50 µg Sambucus ebulus extract for 24 hours, at various concentrations (25, 50, 100, 200, 400 and 500 μg/ml). The cell proliferation rate was assessed by MTT colorimetry assay before and after treatment with the extract.
Results: Immunofluorescent studies showed that neural stem cells expressed specific neural marker; Nestin. The proliferation rate of NSCs increased in the treated groups in comparison to that in the control group. The highest rate of survival was observed when Sambucus ebulus was used at the concentration of 500 μg/ml. (P<0.05).
Conclusion: The results showed that the methanolic extract of Sambucus ebulus can promote proliferation and survival of NCSs in vitro and also after exposure to oxidative stress condition, suggesting its potential beneficial effect on neuroregeneration.
Key Words: Neural stem cells, Sambucus ebulus, Proliferation, Survival.
Received: Jan 13, 2018 Accepted: Apr 11, 2018 |
| first_indexed | 2024-12-13T23:36:38Z |
| format | Article |
| id | doaj.art-00242daef8e541249c7924db6e260a2a |
| institution | Directory Open Access Journal |
| issn | 1560-652X 2345-4040 |
| language | fas |
| last_indexed | 2024-12-13T23:36:38Z |
| publishDate | 2018-08-01 |
| publisher | Kurdistan University of Medical Sciences |
| record_format | Article |
| series | مجله علمی دانشگاه علوم پزشکی کردستان |
| spelling | doaj.art-00242daef8e541249c7924db6e260a2a2022-12-21T23:27:16ZfasKurdistan University of Medical Sciencesمجله علمی دانشگاه علوم پزشکی کردستان1560-652X2345-40402018-08-012332635Effect of Sambucus ebulus extract on neural stem cell prolifration under oxidative stress conditionSara Haratizadeh0Mohammad Ali Ebrahimzadeh1Maryam Nazm Bojnordi2Hatef Ghasemi Hamidabadi3Alireza Abdanipour4Javad Akhtari5 Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Department of Anatomy & Cell Biology, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Department of Anatomy & Cell Biology, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Department of Anatomy, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Immunogenetics Research Center, Department of Physiology and Pharmacology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Background and Aim: Recently, several studies have indicated that the central nervous system has the capacity for endogenous repair. But, the proliferation capacity of endogenous neural stem cells (NSCs) isn’t sufficient for the treatment of neurodegenerative diseases. So, it sounds that stimulation of endogenous NSC proliferation is essential for neuroregeneration. The aim of this study was to examine the effects of Sambucus ebulus extract on the proliferation of neonatal rat hippocampus-derived neural stem cells (NSCs) under oxidative stress condition induced by H2O2. Material and Methods: The NSCs were isolated from neonatal rat hippocampus. To confirm neural characteristics of neural stem cells, the expression of neural-specific marker, Nestin was investigated by immunocytochemistry technique. 5×104 cells were cultured in every well of a 96 well plate and H2O2 was added to induce oxidative stress condition. Then NSCs were exposed to 50 µg Sambucus ebulus extract for 24 hours, at various concentrations (25, 50, 100, 200, 400 and 500 μg/ml). The cell proliferation rate was assessed by MTT colorimetry assay before and after treatment with the extract. Results: Immunofluorescent studies showed that neural stem cells expressed specific neural marker; Nestin. The proliferation rate of NSCs increased in the treated groups in comparison to that in the control group. The highest rate of survival was observed when Sambucus ebulus was used at the concentration of 500 μg/ml. (P<0.05). Conclusion: The results showed that the methanolic extract of Sambucus ebulus can promote proliferation and survival of NCSs in vitro and also after exposure to oxidative stress condition, suggesting its potential beneficial effect on neuroregeneration. Key Words: Neural stem cells, Sambucus ebulus, Proliferation, Survival. Received: Jan 13, 2018 Accepted: Apr 11, 2018http://sjku.muk.ac.ir/article-1-4264-en.htmlneural stem cellssambucus ebulusproliferationsurvival. |
| spellingShingle | Sara Haratizadeh Mohammad Ali Ebrahimzadeh Maryam Nazm Bojnordi Hatef Ghasemi Hamidabadi Alireza Abdanipour Javad Akhtari Effect of Sambucus ebulus extract on neural stem cell prolifration under oxidative stress condition مجله علمی دانشگاه علوم پزشکی کردستان neural stem cells sambucus ebulus proliferation survival. |
| title | Effect of Sambucus ebulus extract on neural stem cell prolifration under oxidative stress condition |
| title_full | Effect of Sambucus ebulus extract on neural stem cell prolifration under oxidative stress condition |
| title_fullStr | Effect of Sambucus ebulus extract on neural stem cell prolifration under oxidative stress condition |
| title_full_unstemmed | Effect of Sambucus ebulus extract on neural stem cell prolifration under oxidative stress condition |
| title_short | Effect of Sambucus ebulus extract on neural stem cell prolifration under oxidative stress condition |
| title_sort | effect of sambucus ebulus extract on neural stem cell prolifration under oxidative stress condition |
| topic | neural stem cells sambucus ebulus proliferation survival. |
| url | http://sjku.muk.ac.ir/article-1-4264-en.html |
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