A novel one-step approach for the construction of yeast surface display Fab antibody libraries

A novel one-step approach for the construction of yeast surface display Fab antibody libraries

Abstract Background Yeast surface display (YSD) has proven to be a versatile platform technology for antibody discovery. However, the construction of antibody Fab libraries typically is a tedious three-step process that involves the generation of heavy chain as well as light chain display plasmids i...

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Bibliographic Details
Main Authors: Simon Rosowski, Stefan Becker, Lars Toleikis, Bernhard Valldorf, Julius Grzeschik, Deniz Demir, Iris Willenbücher, Ramona Gaa, Harald Kolmar, Stefan Zielonka, Simon Krah
Format: Article
Language:English
Published: BMC 2018-01-01
Series:Microbial Cell Factories
Subjects:
Golden Gate Cloning
Yeast surface display
Fab fragment
Antibody discovery
Online Access:http://link.springer.com/article/10.1186/s12934-017-0853-z

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