Down-Regulation of Strigolactone Biosynthesis Gene <i>D17</i> Alters the VOC Content and Increases <i>Sogatella furcifera</i> Infectivity in Rice

<i>DWARF17</i> (<i>D17</i>/<i>HTD1</i>) is a well-defined rice strigolactone (SL) biosynthesis gene that influences rice tiller development and the production of rice. To investigate whether SLs play a role in the regulation of rice’s defense against the white-bac...

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Main Authors: Shanshan Li, Hualiang He, Lin Qiu, Qiao Gao, Youzhi Li, Wenbing Ding
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Agriculture
Subjects:
Online Access:https://www.mdpi.com/2077-0472/13/4/842
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author Shanshan Li
Hualiang He
Lin Qiu
Qiao Gao
Youzhi Li
Wenbing Ding
author_facet Shanshan Li
Hualiang He
Lin Qiu
Qiao Gao
Youzhi Li
Wenbing Ding
author_sort Shanshan Li
collection DOAJ
description <i>DWARF17</i> (<i>D17</i>/<i>HTD1</i>) is a well-defined rice strigolactone (SL) biosynthesis gene that influences rice tiller development and the production of rice. To investigate whether SLs play a role in the regulation of rice’s defense against the white-backed planthopper (WBPH, <i>Sogatella furcifera</i>), we compared a SL-biosynthetic defective mutant (<i>d17</i>) with WT rice plants. Our olfactory bioassay results revealed that WBPHs are attracted to <i>d17</i> plants, which may be attributed to changes in rice volatile substances. Hexanal, a volatile substance, was significantly reduced in the <i>d17</i> plants, and it was demonstrated that it repelled WBPHs at a concentration of 100 μL/L. Compared to the WT plants, WBPH female adults preferred to oviposit on <i>d17</i> plants, where the egg hatching rate was higher. The transcript level analysis of defense-associated genes in the JA and SA pathways showed that the expression of <i>OsJAmyb, OsJAZ8</i>, <i>OsPR1a</i> and <i>OsWRKY62</i> were significantly reduced in <i>d17</i> plants compared to WT plants following WBPH infection. These findings suggest that silencing the strigolactone biosynthesis gene <i>D17</i> weakens defenses against <i>S. furcifera</i> in rice.
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spelling doaj.art-0027ab25110e46c299679afdad41cf2c2023-11-17T17:54:15ZengMDPI AGAgriculture2077-04722023-04-0113484210.3390/agriculture13040842Down-Regulation of Strigolactone Biosynthesis Gene <i>D17</i> Alters the VOC Content and Increases <i>Sogatella furcifera</i> Infectivity in RiceShanshan Li0Hualiang He1Lin Qiu2Qiao Gao3Youzhi Li4Wenbing Ding5College of Plant Protection, Hunan Agricultural University, Changsha 410128, ChinaCollege of Plant Protection, Hunan Agricultural University, Changsha 410128, ChinaCollege of Plant Protection, Hunan Agricultural University, Changsha 410128, ChinaCollege of Plant Protection, Hunan Agricultural University, Changsha 410128, ChinaCollege of Plant Protection, Hunan Agricultural University, Changsha 410128, ChinaCollege of Plant Protection, Hunan Agricultural University, Changsha 410128, China<i>DWARF17</i> (<i>D17</i>/<i>HTD1</i>) is a well-defined rice strigolactone (SL) biosynthesis gene that influences rice tiller development and the production of rice. To investigate whether SLs play a role in the regulation of rice’s defense against the white-backed planthopper (WBPH, <i>Sogatella furcifera</i>), we compared a SL-biosynthetic defective mutant (<i>d17</i>) with WT rice plants. Our olfactory bioassay results revealed that WBPHs are attracted to <i>d17</i> plants, which may be attributed to changes in rice volatile substances. Hexanal, a volatile substance, was significantly reduced in the <i>d17</i> plants, and it was demonstrated that it repelled WBPHs at a concentration of 100 μL/L. Compared to the WT plants, WBPH female adults preferred to oviposit on <i>d17</i> plants, where the egg hatching rate was higher. The transcript level analysis of defense-associated genes in the JA and SA pathways showed that the expression of <i>OsJAmyb, OsJAZ8</i>, <i>OsPR1a</i> and <i>OsWRKY62</i> were significantly reduced in <i>d17</i> plants compared to WT plants following WBPH infection. These findings suggest that silencing the strigolactone biosynthesis gene <i>D17</i> weakens defenses against <i>S. furcifera</i> in rice.https://www.mdpi.com/2077-0472/13/4/842<i>DWARF17</i><i>D17</i>strigolactones<i>Sogatella furcifera</i>rice defense
spellingShingle Shanshan Li
Hualiang He
Lin Qiu
Qiao Gao
Youzhi Li
Wenbing Ding
Down-Regulation of Strigolactone Biosynthesis Gene <i>D17</i> Alters the VOC Content and Increases <i>Sogatella furcifera</i> Infectivity in Rice
Agriculture
<i>DWARF17</i>
<i>D17</i>
strigolactones
<i>Sogatella furcifera</i>
rice defense
title Down-Regulation of Strigolactone Biosynthesis Gene <i>D17</i> Alters the VOC Content and Increases <i>Sogatella furcifera</i> Infectivity in Rice
title_full Down-Regulation of Strigolactone Biosynthesis Gene <i>D17</i> Alters the VOC Content and Increases <i>Sogatella furcifera</i> Infectivity in Rice
title_fullStr Down-Regulation of Strigolactone Biosynthesis Gene <i>D17</i> Alters the VOC Content and Increases <i>Sogatella furcifera</i> Infectivity in Rice
title_full_unstemmed Down-Regulation of Strigolactone Biosynthesis Gene <i>D17</i> Alters the VOC Content and Increases <i>Sogatella furcifera</i> Infectivity in Rice
title_short Down-Regulation of Strigolactone Biosynthesis Gene <i>D17</i> Alters the VOC Content and Increases <i>Sogatella furcifera</i> Infectivity in Rice
title_sort down regulation of strigolactone biosynthesis gene i d17 i alters the voc content and increases i sogatella furcifera i infectivity in rice
topic <i>DWARF17</i>
<i>D17</i>
strigolactones
<i>Sogatella furcifera</i>
rice defense
url https://www.mdpi.com/2077-0472/13/4/842
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