Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing
Abstract Background The leather industry generates huge volume of waste each year. Keratin is the principal constituents of this waste that is resistant to degradation. Some bacteria have the ability to degrade keratin through synthesis of a protease called keratinase that can be used as sources of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-04-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43141-021-00149-8 |
| _version_ | 1797203887744090112 |
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| author | Yeasmin Akter Moonnee Md Javed Foysal Abu Hashem Md Faruque Miah |
| author_facet | Yeasmin Akter Moonnee Md Javed Foysal Abu Hashem Md Faruque Miah |
| author_sort | Yeasmin Akter Moonnee |
| collection | DOAJ |
| description | Abstract Background The leather industry generates huge volume of waste each year. Keratin is the principal constituents of this waste that is resistant to degradation. Some bacteria have the ability to degrade keratin through synthesis of a protease called keratinase that can be used as sources of animal feed and industrial production of biodiesel, biofertilizer, and bioplastic. Majority of the studies focused on keratin degradation using gram-positive bacteria. Not much of studies are currently available on production of keratinase from gram-negative bacteria and selection of best parameters for the maximum production of enzyme. The aim of this study was to isolate and characterize both groups of bacteria from soil for keratinase and optimize the production parameters. Results A total of 50 isolates were used for initial screening of enzyme production in skim milk, casein, and feather meal agar. Out of 50, five isolates showed significantly higher enzyme production in preliminary screening assays. Morphological and biochemical characterization revealed 60% of the isolates as gram-negative bacteria including two highest enzyme-producing isolates. The isolates were identified as Pseudomonas aeruginosa through sequencing of 16S rRNA gene. Maximum production of enzyme from P. aeruginosa YK17 was achieved with 2% chicken feather, beef extract, and ammonium nitrate as organic and inorganic nitrogen sources and glucose as a carbon source. Further analysis revealed that 3% inoculum, 40 °C growth temperature and 72-h incubation, resulted in maximum production of keratinase. Conclusion The overall results showed significant higher production of enzyme by the P. aeruginosa YK17 that can be used for the degradation of recalcitrant keratin waste and chemical dehairing in leather industries, thereby preventing environmental pollution. |
| first_indexed | 2024-04-24T08:26:29Z |
| format | Article |
| id | doaj.art-0028ca690f2d431989393e05e265be19 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:26:29Z |
| publishDate | 2021-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-0028ca690f2d431989393e05e265be192024-04-16T22:08:16ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202021-04-011911910.1186/s43141-021-00149-8Keratinolytic protease from Pseudomonas aeruginosa for leather skin processingYeasmin Akter Moonnee0Md Javed Foysal1Abu Hashem2Md Faruque Miah3Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & TechnologyDepartment of Genetic Engineering & Biotechnology, Shahjalal University of Science & TechnologyMicrobial Biotechnology Division, National Institute of Biotechnology (NIB)Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & TechnologyAbstract Background The leather industry generates huge volume of waste each year. Keratin is the principal constituents of this waste that is resistant to degradation. Some bacteria have the ability to degrade keratin through synthesis of a protease called keratinase that can be used as sources of animal feed and industrial production of biodiesel, biofertilizer, and bioplastic. Majority of the studies focused on keratin degradation using gram-positive bacteria. Not much of studies are currently available on production of keratinase from gram-negative bacteria and selection of best parameters for the maximum production of enzyme. The aim of this study was to isolate and characterize both groups of bacteria from soil for keratinase and optimize the production parameters. Results A total of 50 isolates were used for initial screening of enzyme production in skim milk, casein, and feather meal agar. Out of 50, five isolates showed significantly higher enzyme production in preliminary screening assays. Morphological and biochemical characterization revealed 60% of the isolates as gram-negative bacteria including two highest enzyme-producing isolates. The isolates were identified as Pseudomonas aeruginosa through sequencing of 16S rRNA gene. Maximum production of enzyme from P. aeruginosa YK17 was achieved with 2% chicken feather, beef extract, and ammonium nitrate as organic and inorganic nitrogen sources and glucose as a carbon source. Further analysis revealed that 3% inoculum, 40 °C growth temperature and 72-h incubation, resulted in maximum production of keratinase. Conclusion The overall results showed significant higher production of enzyme by the P. aeruginosa YK17 that can be used for the degradation of recalcitrant keratin waste and chemical dehairing in leather industries, thereby preventing environmental pollution.https://doi.org/10.1186/s43141-021-00149-8BacteriaBiodegradationFermentationLeather industryKeratin waste |
| spellingShingle | Yeasmin Akter Moonnee Md Javed Foysal Abu Hashem Md Faruque Miah Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing Journal of Genetic Engineering and Biotechnology Bacteria Biodegradation Fermentation Leather industry Keratin waste |
| title | Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing |
| title_full | Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing |
| title_fullStr | Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing |
| title_full_unstemmed | Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing |
| title_short | Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing |
| title_sort | keratinolytic protease from pseudomonas aeruginosa for leather skin processing |
| topic | Bacteria Biodegradation Fermentation Leather industry Keratin waste |
| url | https://doi.org/10.1186/s43141-021-00149-8 |
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