Fecal Microbiota Transplantation Protects the Intestinal Mucosal Barrier by Reconstructing the Gut Microbiota in a Murine Model of Sepsis
The gastrointestinal (GI) tract has long been hypothesized to play an integral role in the pathophysiology of sepsis, and gut microbiota (GM) dysbiosis may be the key factor. Previous studies have shown that the gut flora was significantly altered in critically ill patients. This study aimed to obse...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.736204/full |
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| author | Xiaowei Gai Xiaowei Gai Xiaowei Gai Huawei Wang Yaqing Li Haotian Zhao Cong He Zihui Wang Heling Zhao |
| author_facet | Xiaowei Gai Xiaowei Gai Xiaowei Gai Huawei Wang Yaqing Li Haotian Zhao Cong He Zihui Wang Heling Zhao |
| author_sort | Xiaowei Gai |
| collection | DOAJ |
| description | The gastrointestinal (GI) tract has long been hypothesized to play an integral role in the pathophysiology of sepsis, and gut microbiota (GM) dysbiosis may be the key factor. Previous studies have shown that the gut flora was significantly altered in critically ill patients. This study aimed to observe what kind of GM dysbiosis is in the early stage of sepsis and whether the application of fecal microbiota transplantation (FMT) can reconstruct the GM of septic mice and restore its protective function on the intestinal mucosal barrier. The study investigated the effect of FMT on gut microbiota, mucosal barrier function, inflammatory response, and survival in a murine model of sepsis established by cecal ligation and puncture (CLP). It is found that FMT can not only reduce morbidity and mortality and restore the abundance and diversity of the gut flora in septic mice, but can also improve the intestinal barrier function by reducing epithelial cell apoptosis, improving the composition of the mucus layer, upregulating the expression of tight junction proteins, and reducing intestinal permeability and the inflammatory response. After FMT, Lachnospiraceae contributed the most to intestinal protection through enhancement of the L-lysine fermentation pathway. FMT offers a microbe-mediated survival advantage in a murine model of sepsis. Therefore, an improved understanding of the connection between microbiota, and systemic illness may yield new therapeutic strategies for patients with sepsis. |
| first_indexed | 2024-12-13T21:11:34Z |
| format | Article |
| id | doaj.art-002a3ae54c764893bcabb9b26d4e4ab6 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-13T21:11:34Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-002a3ae54c764893bcabb9b26d4e4ab62022-12-21T23:31:20ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-09-011110.3389/fcimb.2021.736204736204Fecal Microbiota Transplantation Protects the Intestinal Mucosal Barrier by Reconstructing the Gut Microbiota in a Murine Model of SepsisXiaowei Gai0Xiaowei Gai1Xiaowei Gai2Huawei Wang3Yaqing Li4Haotian Zhao5Cong He6Zihui Wang7Heling Zhao8Department of Intensive Care Unit, Hebei General Hospital, Shijiazhuang, ChinaGraduate School of Hebei Medical University, Hebei Medical University, Shijiazhuang, ChinaDepartment of Intensive Care Unit, Qinhuangdao Jungong Hospital, Qinhuangdao, ChinaDepartment of Intensive Care Unit, Hebei General Hospital, Shijiazhuang, ChinaDepartment of Infection, Hebei General Hospital, Shijiazhuang, ChinaDepartment of Ultrasound, Hebei General Hospital, Shijiazhuang, ChinaDepartment of Intensive Care Unit, Hebei General Hospital, Shijiazhuang, ChinaDepartment of Intensive Care Unit, Hebei General Hospital, Shijiazhuang, ChinaDepartment of Intensive Care Unit, Hebei General Hospital, Shijiazhuang, ChinaThe gastrointestinal (GI) tract has long been hypothesized to play an integral role in the pathophysiology of sepsis, and gut microbiota (GM) dysbiosis may be the key factor. Previous studies have shown that the gut flora was significantly altered in critically ill patients. This study aimed to observe what kind of GM dysbiosis is in the early stage of sepsis and whether the application of fecal microbiota transplantation (FMT) can reconstruct the GM of septic mice and restore its protective function on the intestinal mucosal barrier. The study investigated the effect of FMT on gut microbiota, mucosal barrier function, inflammatory response, and survival in a murine model of sepsis established by cecal ligation and puncture (CLP). It is found that FMT can not only reduce morbidity and mortality and restore the abundance and diversity of the gut flora in septic mice, but can also improve the intestinal barrier function by reducing epithelial cell apoptosis, improving the composition of the mucus layer, upregulating the expression of tight junction proteins, and reducing intestinal permeability and the inflammatory response. After FMT, Lachnospiraceae contributed the most to intestinal protection through enhancement of the L-lysine fermentation pathway. FMT offers a microbe-mediated survival advantage in a murine model of sepsis. Therefore, an improved understanding of the connection between microbiota, and systemic illness may yield new therapeutic strategies for patients with sepsis.https://www.frontiersin.org/articles/10.3389/fcimb.2021.736204/fullsepsisfecal microbiota transplantationintestinal mucosal barriergut microbiotacritical care |
| spellingShingle | Xiaowei Gai Xiaowei Gai Xiaowei Gai Huawei Wang Yaqing Li Haotian Zhao Cong He Zihui Wang Heling Zhao Fecal Microbiota Transplantation Protects the Intestinal Mucosal Barrier by Reconstructing the Gut Microbiota in a Murine Model of Sepsis Frontiers in Cellular and Infection Microbiology sepsis fecal microbiota transplantation intestinal mucosal barrier gut microbiota critical care |
| title | Fecal Microbiota Transplantation Protects the Intestinal Mucosal Barrier by Reconstructing the Gut Microbiota in a Murine Model of Sepsis |
| title_full | Fecal Microbiota Transplantation Protects the Intestinal Mucosal Barrier by Reconstructing the Gut Microbiota in a Murine Model of Sepsis |
| title_fullStr | Fecal Microbiota Transplantation Protects the Intestinal Mucosal Barrier by Reconstructing the Gut Microbiota in a Murine Model of Sepsis |
| title_full_unstemmed | Fecal Microbiota Transplantation Protects the Intestinal Mucosal Barrier by Reconstructing the Gut Microbiota in a Murine Model of Sepsis |
| title_short | Fecal Microbiota Transplantation Protects the Intestinal Mucosal Barrier by Reconstructing the Gut Microbiota in a Murine Model of Sepsis |
| title_sort | fecal microbiota transplantation protects the intestinal mucosal barrier by reconstructing the gut microbiota in a murine model of sepsis |
| topic | sepsis fecal microbiota transplantation intestinal mucosal barrier gut microbiota critical care |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.736204/full |
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