Axenic Cultivation and Pathogenic Assays of Acanthamoeba Strains Using Physical Parameters
Background: The main goal of the present study was to set up an axenic cultivation of Acanthamoeba and assess the pathogenic ability of T4 genotypes from different clinical and environmental strains of Acanthamoeba using two physical assays. Methods: Sixteen Acanthamoeba isolates including 10 envi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2013-06-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/507 |
| _version_ | 1811190224073523200 |
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| author | M Niyyati H Abedkhojasteh M Salehi Sh Farnia M Rezaeian |
| author_facet | M Niyyati H Abedkhojasteh M Salehi Sh Farnia M Rezaeian |
| author_sort | M Niyyati |
| collection | DOAJ |
| description | Background: The main goal of the present study was to set up an axenic cultivation of Acanthamoeba and assess the pathogenic ability of T4 genotypes from different clinical and environmental strains of Acanthamoeba using two physical assays.
Methods: Sixteen Acanthamoeba isolates including 10 environmental and 6 clinical strains were cul-tured axenically. Axenic cultivation was performed using Proteosepepton, yeast extract and glucose medium and TY-I-S33culture. Pathogenic survey was done using osmotolerance and thermotoler-ance assay. Briefly, differentosmolarity (0.5 M and 1 M) of non-nutrient agar plates were performed. One hundred fiftyμl of axenic culture were collected and were inoculated in 1% agar medium. For thermotolerance assay 150 μl of amoebas from axenic culture were divided into fresh culture me-diums. Cultures were incubated at 37oC and 42 oC. All plates were monitored for 24 h, 48 h and 72 h.
Results: Overall, 16 strains of Acanthamoeba isolates previously genotyped as T4 were cultivated axenically after several months. Thermotolerance and osmotolerance assay revealed that all of clinical strains, soil and animal feces strains were highly pathogenic isolates. Two dust and water strains did not grow at high temperature (42 oC) and osmolarity (1.5 M) and thus they were classified as weak pathogens.
Conclusion: Most of T4 genotypes are highly pathogenic organisms. This is an important finding since Acanthamoeba belonging to T4 type is the predominate genotype in environmental and clinical samples. The presence of highly pathogenic Acanthamoeba may pose a risk within susceptible people |
| first_indexed | 2024-04-11T14:47:45Z |
| format | Article |
| id | doaj.art-002b223c39884e8ebaa94413a1c72fb1 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-04-11T14:47:45Z |
| publishDate | 2013-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-002b223c39884e8ebaa94413a1c72fb12022-12-22T04:17:34ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2013-06-0182Axenic Cultivation and Pathogenic Assays of Acanthamoeba Strains Using Physical ParametersM Niyyati0H Abedkhojasteh1M Salehi2Sh Farnia3M Rezaeian4Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran,IranDepartment of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran,IranDepartment of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran,IranDepartment of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran,Iran AND Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, IranBackground: The main goal of the present study was to set up an axenic cultivation of Acanthamoeba and assess the pathogenic ability of T4 genotypes from different clinical and environmental strains of Acanthamoeba using two physical assays. Methods: Sixteen Acanthamoeba isolates including 10 environmental and 6 clinical strains were cul-tured axenically. Axenic cultivation was performed using Proteosepepton, yeast extract and glucose medium and TY-I-S33culture. Pathogenic survey was done using osmotolerance and thermotoler-ance assay. Briefly, differentosmolarity (0.5 M and 1 M) of non-nutrient agar plates were performed. One hundred fiftyμl of axenic culture were collected and were inoculated in 1% agar medium. For thermotolerance assay 150 μl of amoebas from axenic culture were divided into fresh culture me-diums. Cultures were incubated at 37oC and 42 oC. All plates were monitored for 24 h, 48 h and 72 h. Results: Overall, 16 strains of Acanthamoeba isolates previously genotyped as T4 were cultivated axenically after several months. Thermotolerance and osmotolerance assay revealed that all of clinical strains, soil and animal feces strains were highly pathogenic isolates. Two dust and water strains did not grow at high temperature (42 oC) and osmolarity (1.5 M) and thus they were classified as weak pathogens. Conclusion: Most of T4 genotypes are highly pathogenic organisms. This is an important finding since Acanthamoeba belonging to T4 type is the predominate genotype in environmental and clinical samples. The presence of highly pathogenic Acanthamoeba may pose a risk within susceptible peoplehttps://ijpa.tums.ac.ir/index.php/ijpa/article/view/507AcanthamoebaAxenic cultivationPathogenicity |
| spellingShingle | M Niyyati H Abedkhojasteh M Salehi Sh Farnia M Rezaeian Axenic Cultivation and Pathogenic Assays of Acanthamoeba Strains Using Physical Parameters Iranian Journal of Parasitology Acanthamoeba Axenic cultivation Pathogenicity |
| title | Axenic Cultivation and Pathogenic Assays of Acanthamoeba Strains Using Physical Parameters |
| title_full | Axenic Cultivation and Pathogenic Assays of Acanthamoeba Strains Using Physical Parameters |
| title_fullStr | Axenic Cultivation and Pathogenic Assays of Acanthamoeba Strains Using Physical Parameters |
| title_full_unstemmed | Axenic Cultivation and Pathogenic Assays of Acanthamoeba Strains Using Physical Parameters |
| title_short | Axenic Cultivation and Pathogenic Assays of Acanthamoeba Strains Using Physical Parameters |
| title_sort | axenic cultivation and pathogenic assays of acanthamoeba strains using physical parameters |
| topic | Acanthamoeba Axenic cultivation Pathogenicity |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/507 |
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