Evaluation of the Protective Effect of Crocin on the Development of Embryos from In Vitro Fertilization (IVF) in Busulphan-treated Mice
Background & Objective: Busulfan (BSF) besides the therapeutic effects, performs oxidative stress and decreasing fertilizing capacity. Therefore, the present study was aimed to evaluate the protective effects of Crocin, on in vitro fertilization (IVF) process in busolfan treated mice. Materials...
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| Format: | Article |
| Language: | English |
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Fasa University of Medical Sciences
2019-04-01
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| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-1740-en.pdf |
| _version_ | 1797679599831744512 |
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| author | Hamid Reza Hasanzadeh Khanmiri Rasoul Shahrooz Shapour Hasanzadeh Golam Reza Najafi |
| author_facet | Hamid Reza Hasanzadeh Khanmiri Rasoul Shahrooz Shapour Hasanzadeh Golam Reza Najafi |
| author_sort | Hamid Reza Hasanzadeh Khanmiri |
| collection | DOAJ |
| description | Background & Objective: Busulfan (BSF) besides the therapeutic effects, performs oxidative stress and decreasing fertilizing capacity. Therefore, the present study was aimed to evaluate the protective effects of Crocin, on in vitro fertilization (IVF) process in busolfan treated mice.
Materials & Methods: In this study eighteen mature female mice (25g), were divided into 6 groups and treated for 21 days. The control group received alone resolvent of busulfan (0.1 ml) intraperitoneally (IP) single dose, and sham control group received BSF alone (10mg/kg, IP/single dose) and experimental groups 1, 2, 3 received BSF(10mg/kg/single dose) with Crocin (100, 200, 400mg/kg/day, IP). Positive control group received alone Crocin (400mg/kg, IP/day). At the end of the treatment period, animals were euthanized and after performing the IVF, early embryo development was evaluated. The obtained data were compared between all groups and analyzed using one-way ANOVA, followed by Bonferroni post-hoc test. A P-value <0.05 was considered significant.
Results: Results showed that, the administration of crocin along with busulfan increased significantly oocyte quality, fertilization rate, pre-implantation embryonic development and quality of embryo in comparison to group that received busulfan (P<0.05). In addition, in the group that received crocin any toxicity has not been observed.
Conclusion: The present study indicated that crocin can protect female fertility potential against busulfan induced damages. |
| first_indexed | 2024-03-11T23:17:08Z |
| format | Article |
| id | doaj.art-0030621ff7314de8bdb10f503de8313c |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:17:08Z |
| publishDate | 2019-04-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-0030621ff7314de8bdb10f503de8313c2023-09-20T21:26:04ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232019-04-019113141323Evaluation of the Protective Effect of Crocin on the Development of Embryos from In Vitro Fertilization (IVF) in Busulphan-treated MiceHamid Reza Hasanzadeh Khanmiri0Rasoul Shahrooz1Shapour Hasanzadeh2Golam Reza Najafi3 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Background & Objective: Busulfan (BSF) besides the therapeutic effects, performs oxidative stress and decreasing fertilizing capacity. Therefore, the present study was aimed to evaluate the protective effects of Crocin, on in vitro fertilization (IVF) process in busolfan treated mice. Materials & Methods: In this study eighteen mature female mice (25g), were divided into 6 groups and treated for 21 days. The control group received alone resolvent of busulfan (0.1 ml) intraperitoneally (IP) single dose, and sham control group received BSF alone (10mg/kg, IP/single dose) and experimental groups 1, 2, 3 received BSF(10mg/kg/single dose) with Crocin (100, 200, 400mg/kg/day, IP). Positive control group received alone Crocin (400mg/kg, IP/day). At the end of the treatment period, animals were euthanized and after performing the IVF, early embryo development was evaluated. The obtained data were compared between all groups and analyzed using one-way ANOVA, followed by Bonferroni post-hoc test. A P-value <0.05 was considered significant. Results: Results showed that, the administration of crocin along with busulfan increased significantly oocyte quality, fertilization rate, pre-implantation embryonic development and quality of embryo in comparison to group that received busulfan (P<0.05). In addition, in the group that received crocin any toxicity has not been observed. Conclusion: The present study indicated that crocin can protect female fertility potential against busulfan induced damages.http://jabs.fums.ac.ir/article-1-1740-en.pdfbusulfanovarycrocinmicein vitro fertilization |
| spellingShingle | Hamid Reza Hasanzadeh Khanmiri Rasoul Shahrooz Shapour Hasanzadeh Golam Reza Najafi Evaluation of the Protective Effect of Crocin on the Development of Embryos from In Vitro Fertilization (IVF) in Busulphan-treated Mice Journal of Advanced Biomedical Sciences busulfan ovary crocin mice in vitro fertilization |
| title | Evaluation of the Protective Effect of Crocin on the Development of Embryos from In Vitro Fertilization (IVF) in Busulphan-treated Mice |
| title_full | Evaluation of the Protective Effect of Crocin on the Development of Embryos from In Vitro Fertilization (IVF) in Busulphan-treated Mice |
| title_fullStr | Evaluation of the Protective Effect of Crocin on the Development of Embryos from In Vitro Fertilization (IVF) in Busulphan-treated Mice |
| title_full_unstemmed | Evaluation of the Protective Effect of Crocin on the Development of Embryos from In Vitro Fertilization (IVF) in Busulphan-treated Mice |
| title_short | Evaluation of the Protective Effect of Crocin on the Development of Embryos from In Vitro Fertilization (IVF) in Busulphan-treated Mice |
| title_sort | evaluation of the protective effect of crocin on the development of embryos from in vitro fertilization ivf in busulphan treated mice |
| topic | busulfan ovary crocin mice in vitro fertilization |
| url | http://jabs.fums.ac.ir/article-1-1740-en.pdf |
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